Ultrasonic perfusion imaging of peripheral vascular disease

周围血管疾病的超声灌注成像

基本信息

批准号：
10413895
负责人：
Azra Alizad
金额：
$ 51.75万
依托单位：
UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-08-05 至 2024-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10413895
关键词：
3-Dimensional Anatomy Angiography Animals Ankle Blood Blood Vessels Blood flow Cardiovascular system Clinic Clinical Clinical Research Collaborations Color Complex Computer software Contrast Media Data Development Diabetic mouse Diagnosis Diagnostic Dimensions Disease Progression Doppler Ultrasound Erythrocytes Etiology Evaluation Event Exercise Therapy Failure Family suidae Frequencies Gene Expression Goals Hindlimb Human Image Imaging Device Imaging Techniques Injectable Ischemia Legal patent Lesion Life Style Link Measurement Measures Medical Methods Microspheres Microvascular Dysfunction Monitor Mus Muscle Names Nitric Oxide Operative Surgical Procedures Patient Monitoring Patients Pattern Performance Perfusion Peripheral Peripheral Vascular Diseases Peripheral arterial disease Physiologic pulse Pre-Clinical Model Production Property Radioactive Recovery Research Research Design Resolution Risk Sampling Sensitivity and Specificity Signal Transduction Source Staging Surgeon Symptoms Techniques Technology Therapeutic Time Tissues Translating Translations Ultrasonics Update Work angiogenesis base blood perfusion cardiovascular health cell motility claudication cohort cost design diabetic diabetic patient diagnostic tool disability disease diagnosis experimental study imaging approach improved in vivo in vivo imaging indexing instrument melanoma mouse model multimodality muscle metabolism novel nuclear imaging perfusion imaging phantom model porcine model quantitative imaging research study response simulation spatiotemporal success tool treatment response

项目摘要

Project Summary Our 4-yr project aims to develop a new ultrasonic Doppler method named Higher-Order Perfusion Estimation (HOPE) imaging. Applications enabled by this technology are routine assessments of microvascular disease progression in diabetic patients with symptoms of peripheral artery disease (PAD). Technical advances include new ultrasonic echo sampling and filtering techniques that significantly increase the sensitivity and specificity of standard sonographic instruments to spatially disorganized patterns of red-blood-cell movement without the addition of contrast media. Sensitivity to slowly perfusing blood is increased by transmitting a sparse regular sequence of Doppler pulses over long durations (1-10s). To counter a concomitant increase in clutter-signal power in perfusing-blood frequency channels, spatiotemporal echo acquisitions are first rearranged into 3-D data arrays and a 3-D singular-value decomposition (3D-SVD) clutter filter is formed for each experiment. Our approach now successfully separates weak perfusing-blood echoes from other echo-signal sources in the peripheral vasculature because of the typically narrow eigen-bandwidth of clutter echoes in peripheral muscle. Preliminary results using echo simulation, microchannel flow phantoms, and preclinical models of mouse ischemic hindlimb and melanoma lesions demonstrate that our method is very well designed for monitoring steady peripheral microvascular flow patterns using commercial ultrasonic instruments (with software updates). Four aims are proposed to demonstrate the utility of HOPE imaging (both power and color-flow) for measuring blood flow and perfusion. Aim 1 expands preliminary studies in mouse models to evaluate perfusion measurement sensitivity at 12-24 MHz in diabetic animals, and to uncover mechanisms of the angiogenic response of tissues to sudden ischemia. Aim 2 continues development of HOPE imaging by improving perfusing- blood echo sensitivity and clutter-filter performance under more general imaging conditions (viz., broad eigen- bandwidth clutter and 3-D spatially varying perfusion). Aim 3 focuses on a progressively ischemic pig model using 5-12 MHz HOPE imaging, MR angiography, and radioactive microsphere techniques to calibrate and compare HOPE imaging results with standard clinical approaches. Aim 4 is a 4-yr, 150-patient study designed to evaluate the diagnostic performance of HOPE imaging at assessing PAD. The overall project aims to develop existing ultrasonic instruments into highly-effect tools for evaluating microvascular changes leading to common disabilities and major cardiovascular events. The three in vivo studies proposed in this plan are designed to develop and evaluate HOPE imaging specifically for PAD diagnosis, staging, and therapeutic monitoring.

项目摘要我们的4年项目旨在开发一种名为“高阶灌注估计”的新超声多普勒方法（希望）成像。该技术启用的应用是对微血管疾病的常规评估糖尿病患者患有周围动脉疾病症状（PAD）的进展。技术进步包括新的超声波回声采样和过滤技术，可显着提高标准超声仪，用于无效的红血球运动的空间杂乱无章的模式添加对比媒体。通过传输稀疏的常规来增加对缓慢灌注血液的敏感性多普勒脉冲的序列在长时间（1-10s）中。应对杂物信号的同时增加首先将时空回声采集重新排列到3-D数据为每个实验形成阵列和3-D单数值分解（3D-SVD）混乱过滤器。我们的现在，方法成功地将弱的灌注血回波与其他回声信号来源分开外围脉管系统通常是外围肌肉中杂物回波的狭窄欧吉人带宽。使用回声模拟，微通道流幻象和小鼠的临床前模型的初步结果缺血性后肢和黑色素瘤病变表明，我们的方法非常适合监测使用商业超声仪器（带有软件更新）稳定的周围微血管流动模式。提出了四个目标，以证明希望成像的实用性（功率和颜色流）用于测量血流和灌注。 AIM 1扩展小鼠模型中的初步研究以评估灌注糖尿病动物中12-24 MHz的测量灵敏度，并发现血管生成的机制组织对突然缺血的反应。 AIM 2通过改善完善 - 继续发展希望成像在更一般的成像条件下，血液回声敏感性和混乱过滤器的性能（即，宽阔的特征带宽混乱和3-D在空间变化的灌注）。 AIM 3专注于逐渐缺血模型使用5-12 MHz希望成像，MR血管造影和放射性微球技术进行校准和将希望成像结果与标准临床方法进行比较。 AIM 4是设计的4年，150名患者的研究评估评估垫时希望成像的诊断性能。整个项目旨在发展现有的超声仪器进入高度效应工具，用于评估微血管变化，导致常见残疾和主要心血管事件。本计划中提出的三个体内研究旨在专门为PAD诊断，分期和治疗性监测而开发和评估希望成像。

项目成果

期刊论文数量（6）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Monitoring Muscle Perfusion in Rodents During Short-Term Ischemia Using Power Doppler Ultrasound.

使用能量多普勒超声监测啮齿类动物短期缺血期间的肌肉灌注。

DOI：
10.1016/j.ultrasmedbio.2023.02.013
发表时间：
2023
期刊：
Ultrasound in medicine & biology
影响因子：
2.9
作者：
Babaei,Somaye;Dai,Bingze;Abbey,CraigK;Ambreen,Yamenah;Dobrucki,WawrzyniecL;Insana,MichaelF
通讯作者：
Insana,MichaelF

Contrast-free ultrasound imaging for blood flow assessment of the lower limb in patients with peripheral arterial disease: a feasibility study.