500 MHz NMR Spectrometer Upgrade and Cryoprobe

500 MHz NMR 波谱仪升级和冷冻探针

基本信息

批准号：
10412824
负责人：
Nicholas Fraser Breen
金额：
$ 60万
依托单位：
UNIVERSITY OF WASHINGTON
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-04-01 至 2023-03-31
项目状态：
已结题

项目摘要

PROJECT SUMMARY/ABSTRACT We request the funding to purchase a Bruker NEO 500 MHz NMR spectrometer, equipped with a Prodigy (nitrogen boiloff) cryoprobe and a SampleCase 24-position sample changer. The existing NMR capabilities at the University of Washington, Seattle (UW) are increasingly both obsolete and unreliable, and indeed we recently had to decommission one instrument where neither the electronics nor magnet could be economically repaired. The technical limitations of much of our remaining instrumentation are slowing or blocking many lines of investigation on NIH-funded research in multiple departments. The proposed system will simultaneously improve reliability and efficiency by replacing our fastest-failing instrument and expand our research capabilities with advances in NMR technology that our decades-old equipment cannot provide. These include: (1) drastically increased sensitivity, 2-3 fold, across a range of nuclei ranging from 15N to 19F – an enhancement particularly valuable for 31P and 19F, nuclei of wide- ranging research utility that are badly underserved with existing equipment, and for which this will become the most sensitive instrument available on campus by a wide margin; (2) a combination of an automatic sample changer, automatic probe tuning and shimming, and automation software, which collectively provide users with highly efficient, hands-off sample handling to supply continued, productive instrument use during unattended hours; and (3) dynamic, wide-ranged temperature control, with experimental variability over a broad range to study reaction dynamics, and 0.1°C-precision stability to hold delicate biochemical samples at a safe, consistent temperature during storage and data acquisition. From small molecules to large proteins, NMR is a vital tool for determining structure, kinetics, and molecular interactions, relied upon by dozens of research groups on campus. This instrument will supply crucial support to full range of projects related to human health, including the development of new biomedical screening technologies, new synthetic pathways for pharmaceutical developments, mechanistic investigations of enzymatic tumor suppressors, metabolomic nutritional studies bolstering the recovery of critically ill patients, and many more.

项目概要/摘要我们请求资金购买配备 Prodigy 的 Bruker NEO 500 MHz NMR 波谱仪（氮气蒸发）冷冻探针和 SampleCase 24 位样品更换器现有 NMR。华盛顿大学西雅图分校 (UW) 的能力越来越过时且不可靠，事实上，我们最近不得不退役一台既没有电子设备也没有磁铁的仪器我们剩余的许多仪器的技术限制是可以经济地修复的。减缓或阻止对 NIH 资助的多个部门研究的许多调查。所提出的系统将通过替换我们故障最快的系统来同时提高可靠性和效率仪器并通过 NMR 技术的进步扩展我们的研究能力，该技术已有数十年历史这些包括：(1) 在一定范围内显着提高 2-3 倍的灵敏度。原子核范围从 15N 到 19F – 这种增强对于 31P 和 19F 来说特别有价值，宽原子核范围研究公用事业，现有设备严重不足，并且这将成为 (2)自动组合进样器、自动探头调谐和匀场以及自动化软件，这些统称为为用户提供高效、无需干预的样品处理，以提供持续、高效的仪器在无人值守的情况下使用；(3) 动态、宽范围的温度控制，具有实验性广泛范围内的变异性可用于研究反应动力学，0.1°C 精度的稳定性可保持微妙的变化生化样品在储存和采集过程中保持安全、一致的温度。从分子到大蛋白质，NMR 是确定结构、动力学和分子结构的重要工具。校园内数十个研究小组所依赖的互动，该工具将提供至关重要的信息。支持与人类健康相关的全方位项目，包括开发新的生物医学筛选技术、药物开发的新合成途径、机理酶促肿瘤抑制因子的研究、支持康复的代谢组学营养研究危重病人等等。