500 MHz NMR Spectrometer Upgrade and Cryoprobe

500 MHz NMR 波谱仪升级和冷冻探针

• 批准号: 10412824
• 负责人: Nicholas Fraser Breen
• 金额: $ 60万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-04-01 至 2023-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10412824
• 关键词: Automation; Binding; Biochemical; Cell Nucleus; Computer software; Critical Illness; Development; Electronics; Equipment; Funding; Hand; Health; Hour; Human; Investigation; Kinetics; NMR Spectroscopy; Nitrogen; Nutritional Study; Pathway interactions; Patients; Pharmacologic Substance; Positioning Attribute; Proteins; Reaction; Recovery; Research; Sampling; Structure; System; Technology; Temperature; Tumor Suppressor Proteins; United States National Institutes of Health; Universities; Washington; data acquisition; improved; instrument; instrumentation; metabolomics; repaired; screening; small molecule; tool; Automation; Binding; Biochemical; Cell Nucleus; Computer software; Critical Illness; Development; Electronics; Equipment; Funding; Hand; Health; Hour; Human; Investigation; Kinetics; NMR Spectroscopy; Nitrogen; Nutritional Study; Pathway interactions; Patients; Pharmacologic Substance; Positioning Attribute; Proteins; Reaction; Recovery; Research; Sampling; Structure; System; Technology; Temperature; Tumor Suppressor Proteins; United States National Institutes of Health; Universities; Washington; data acquisition; improved; instrument; instrumentation; metabolomics; repaired; screening; small molecule; tool

PROJECT SUMMARY/ABSTRACT We request the funding to purchase a Bruker NEO 500 MHz NMR spectrometer, equipped with a Prodigy (nitrogen boiloff) cryoprobe and a SampleCase 24-position sample changer. The existing NMR capabilities at the University of Washington, Seattle (UW) are increasingly both obsolete and unreliable, and indeed we recently had to decommission one instrument where neither the electronics nor magnet could be economically repaired. The technical limitations of much of our remaining instrumentation are slowing or blocking many lines of investigation on NIH-funded research in multiple departments. The proposed system will simultaneously improve reliability and efficiency by replacing our fastest-failing instrument and expand our research capabilities with advances in NMR technology that our decades-old equipment cannot provide. These include: (1) drastically increased sensitivity, 2-3 fold, across a range of nuclei ranging from 15N to 19F – an enhancement particularly valuable for 31P and 19F, nuclei of wide- ranging research utility that are badly underserved with existing equipment, and for which this will become the most sensitive instrument available on campus by a wide margin; (2) a combination of an automatic sample changer, automatic probe tuning and shimming, and automation software, which collectively provide users with highly efficient, hands-off sample handling to supply continued, productive instrument use during unattended hours; and (3) dynamic, wide-ranged temperature control, with experimental variability over a broad range to study reaction dynamics, and 0.1°C-precision stability to hold delicate biochemical samples at a safe, consistent temperature during storage and data acquisition. From small molecules to large proteins, NMR is a vital tool for determining structure, kinetics, and molecular interactions, relied upon by dozens of research groups on campus. This instrument will supply crucial support to full range of projects related to human health, including the development of new biomedical screening technologies, new synthetic pathways for pharmaceutical developments, mechanistic investigations of enzymatic tumor suppressors, metabolomic nutritional studies bolstering the recovery of critically ill patients, and many more.

项目摘要/摘要 我们要求资金购买Bruker Neo 500 MHz NMR光谱仪，配备了神童 （氮沸腾）冷冻探针和24位样品更换器。现有的NMR 华盛顿大学西雅图大学（UW）的能力越来越过时和不可靠， 实际上，我们最近不得不退役一种仪器 可以在经济上修复。我们剩余仪器的技术局限性是 放缓或阻止多个部门的NIH资助研究的许多投资。这 拟议的系统将通过替换我们最快的失败来提高可靠性和效率 通过NMR技术的进步来仪器并扩展我们的研究能力，这是我们数十年历史的 设备无法提供。其中包括：（1）在一个范围内急剧提高灵敏度，2-3倍 核的范围从15n到19f - 这是31便士和19F特别有价值的增强，核的核 与现有设备不满意的研究实用程序，这将成为 校园中最敏感的仪器宽阔的边缘； （2）自动的组合 示例更换器，自动探针调整和弹药以及自动化软件，该软件共同 为用户提供高效，可开关的样品处理以继续，产品仪器 在无人值守的时间内使用； （3）具有实验性的动态，广泛的温度控制 在广泛范围内研究反应动力学的可变性，以及0.1°C的稳定性，以保持精致 在存储和数据采集期间，在安全，一致的温度下的生化样品。来自小 NMR是大蛋白的分子，是确定结构，动力学和分子的重要工具 相互作用，校园内数十个研究小组依靠。该乐器将提供至关重要的 支持与人类健康有关的全部项目，包括开发新的生物医学 筛选技术，药物开发的新合成途径，机械 研究酶促补充剂，代谢组营养研究的研究加强了恢复 重症患者的病情等等。