Core 2: Mouse, Cell and Protein Support, Data Management and Biostatistics Core

核心 2：小鼠、细胞和蛋白质支持、数据管理和生物统计学核心

• 批准号: 10407556
• 负责人: Ravichandran Ramasamy
• 金额: $ 83.5万
• 依托单位: NEW YORK UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-08-01 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10407556
• 关键词: Biometry; Biostatistics Core; Bone Marrow; Breeding; Budgets; Cardiac Myocytes; Cell Communication; Cell Separation; Cells; Computer software; Diabetes Mellitus; Endothelial Cells; Ensure; Equipment; Floor; Funding; Goals; Health; Helicobacter; Housing; Instruction; Laboratories; Letters; Mus; National Heart, Lung, and Blood Institute; Norovirus; Operative Surgical Procedures; Postoperative Period; Preparation; Procedures; Proteins; Reperfusion Injury; Research Personnel; Resources; Science; Signal Transduction; Site; Universities; Writing; base; data management; experience; ischemic injury; macrophage; medical schools; mutant; programs; Biometry; Biostatistics Core; Bone Marrow; Breeding; Budgets; Cardiac Myocytes; Cell Communication; Cell Separation; Cells; Computer software; Diabetes Mellitus; Endothelial Cells; Ensure; Equipment; Floor; Funding; Goals; Health; Helicobacter; Housing; Instruction; Laboratories; Letters; Mus; National Heart, Lung, and Blood Institute; Norovirus; Operative Surgical Procedures; Postoperative Period; Preparation; Procedures; Proteins; Reperfusion Injury; Research Personnel; Resources; Science; Signal Transduction; Site; Universities; Writing; base; data management; experience; ischemic injury; macrophage; medical schools; mutant; programs

Project Summary: Scientific Core The overall goal of Core 2 is to provide the key resources, including mice bred-in-house; primary cells from mice (endothelial cells, cardiomyocytes and bone marrow derived macrophages); and surgical procedures on mice required for two of the three Projects. Further, this Core provides proteins/mutant proteins of RAGE, DIAPH1 and MFN2 that are required for the three Projects. In addition, the entire Program Project is served by the data management and biostatistical component of this Core. The rationale to centralize these key activities is to ensure rigorous data management and biostatistical support, resource and procedural consistency, and maximal and most efficient use of resources throughout the Program Project. All three Projects will use this Core 2 over all five years of the Program Project. The five Specific Aims of this core are as follows: Specific Aim 1 will provide data management and biostatistical support; Specific Aim 2 will perform the breeding of the mice to be employed in this Program project; Specific Aim 3 will perform the surgical procedures to induce ischemia and reperfusion injuries to mice in this Program Project; Specific Aim 4 will isolate and characterize the primary macrophages, cardiomyocytes and endothelial cells to be employed in this Program Project ; Specific Aim 5 will prepare and characterize purified proteins and site-directed mutants of RAGE, DIAPH1 and MFN2 to be employed in this Program Project. AIMS 1-4 are based at the NYU School of Medicine/NYU Langone Health, while AIM 5 takes place at the SUNY at Albany. All of the necessary facilities and equipment are available at NYU School of Medicine/NYU Langone Health and at the SUNY at Albany to successfully carry out the proposed studies. The Core leader and Core Co-Investigator have all of the necessary expertise and experience to oversee these core functions. Letters are provided and appended to this Core indicating that there is NO OVERLAP of the five specific aims of this Core with NYU- or Albany-institutionally funded-Cores. This core is in full compliance with the instructions for scientific cores within NHLBI Program Projects. Core 2 serves all three projects of the Program over all 5 years of the Program Project.

项目摘要：科学核心 Core 2的总体目标是提供关键资源，包括内部繁殖的小鼠；来自小鼠的原代细胞 （内皮细胞，心肌细胞和骨髓衍生的巨噬细胞）；和小鼠的手术程序 这三个项目中的两个需要。此外，该核心提供愤怒的蛋白质/突变蛋白，Diaph1 和三个项目所需的MFN2。此外，整个程序项目还由数据服务 该核心的管理和生物统计组成部分。集中这些关键活动的理由是 确保严格的数据管理和生物统计学支持，资源和程序一致性以及 在整个计划项目中，最大和最有效地利用资源。这三个项目都将使用此 核心2在该计划项目的所有五年中。该核心的五个具体目标如下：特定目标 1将提供数据管理和生物统计支持；特定的目标2将执行小鼠的繁殖 被雇用在这个计划项目中；特定的目标3将执行诱导缺血的手术程序 在该计划项目中，小鼠的再灌注受伤；特定的目标4将隔离并表征初级 该计划项目中要使用的巨噬细胞，心肌细胞和内皮细胞；具体目标5将 准备并表征纯化的蛋白质和rage，diaph1和mfn2的位置定向的突变体 在此计划项目中使用。 AIMS 1-4位于纽约大学医学院/NYU Langone Health， AIM 5发生在奥尔巴尼的纽约州立大学。所有必要的设施和设备都可以在 纽约大学医学院/NYU Langone Health和在奥尔巴尼的纽约州立大学，成功执行了拟议的 研究。核心领导者和核心共同投资者拥有所有必要的专业知识和经验来监督 这些核心功能。提供了字母并将其附加到此核心上，表明没有重叠 该核心的五个特定目标是NYU或Albany机构资助的核心。这个核心完全合规 在NHLBI计划项目中的科学核心指示中。核心2为所有三个项目提供 计划在计划项目的所有5年中。