Development of a Bio-tissue Oxygenation Nanophosphor Enabled Sensing (BONES) system for Quantifying Hypoxia in Bone Marrow

开发用于量化骨髓缺氧的生物组织氧化纳米磷传感 (BONES) 系统

基本信息

批准号：
10408542
负责人：
Wenbing Yun
金额：
$ 86.65万
依托单位：
SIGRAY, INC.
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-06-01 至 2024-02-29
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10408542
关键词：
3-Dimensional Address Affect Algorithms Area Biomedical Research Biopsy Blood Vessels Bone Marrow Bone Marrow Transplantation Cancer Patient Cancer Relapse Cells Chemicals Chronic Kidney Failure Clinical Collaborations Data Detection Development Disease Dose Environment Fiber Film Heterogeneity Human body Hypoxia Image Imaging Techniques Institution Light Magnetic Resonance Imaging Malignant Neoplasms Malignant neoplasm of prostate Measurement Measures Metastatic Neoplasm to the Bone Methods Microscopy Modality Molecular Monitor Morphology Nature Neoplasm Metastasis Noise Organ Transplantation Oxygen Penetration Performance Phase Photons Production Radiation Dose Unit Resolution Roentgen Rays Sampling Scanning Signal Transduction Site Small Business Technology Transfer Research Spatial Design Surface System Techniques Therapeutic Thick Time Tissues Visible Radiation X-Ray Computed Tomography adult stem cell base bone cancer cell cancer site deep learning deep learning algorithm denoising design and construction detector high resolution imaging image processing image reconstruction imaging system improved insight luminescence malignant breast neoplasm molecular imaging multidisciplinary nanophosphor phosphorescence pre-clinical prevent prototype quantum response scaffold stem cell growth stem cells therapeutic effectiveness tissue oxygenation tomography transmission process tumor two-photon

3-Dimensional Address Affect Algorithms Area Biomedical Research Biopsy Blood Vessels Bone Marrow Bone Marrow Transplantation Cancer Patient Cancer Relapse Cells Chemicals Chronic Kidney Failure Clinical Collaborations Data Detection Development Disease Dose Environment Fiber Film Heterogeneity Human body Hypoxia Image Imaging Techniques Institution Light Magnetic Resonance Imaging Malignant Neoplasms Malignant neoplasm of prostate Measurement Measures Metastatic Neoplasm to the Bone Methods Microscopy Modality Molecular Monitor Morphology Nature Neoplasm Metastasis Noise Organ Transplantation Oxygen Penetration Performance Phase Photons Production Radiation Dose Unit Resolution Roentgen Rays Sampling Scanning Signal Transduction Site Small Business Technology Transfer Research Spatial Design Surface System Techniques Therapeutic Thick Time Tissues Visible Radiation X-Ray Computed Tomography adult stem cell base bone cancer cell cancer site deep learning deep learning algorithm denoising design and construction detector high resolution imaging image processing image reconstruction imaging system improved insight luminescence malignant breast neoplasm molecular imaging multidisciplinary nanophosphor phosphorescence pre-clinical prevent prototype quantum response scaffold stem cell growth stem cells therapeutic effectiveness tissue oxygenation tomography transmission process tumor two-photon

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项目摘要

Project Summary/Abstract Low oxygen (hypoxic) environments are known to be important for maintaining the small number of adult stem cells in the human body, such as in bone marrow. These conditions are also believed to enable dormant cancer cells to survive and metastasize years or decades after the original tumor has been destroyed and the reason why bone marrow is one of the most common sites of cancer metastasis. Understanding of these conditions can drive the development of 3D cellular scaffolds for growing stem cells ex vivo, thus reducing the burden on requiring bone marrow transplants, and for developing therapeutics that prevent cancer relapse. This project proposes to develop the first quantitative oxygen tomographic imaging system called BONES (Bio-tissue Oxygenation Nanophosphor Enabled Sensing) to address the critical need for high resolution imaging of oxygen concentrations in hypoxic (low oxygen) tissues such as bone marrow. The technique is based on developments in x-ray luminescence computed tomography, an emerging molecular imaging technique capable of achieving cellular level resolution and high sensitivities. The approach uses x-rays to excite oxygen-sensitive nanophosphors that emit near-infrared photons to finally enable 3D oxygen measurements in deep bone marrow. Because the technique requires a multidisciplinary team with x-ray expertise, nanophosphor expertise, near-infrared detection expertise, and algorithms for quantifying the concentrations and minimizing dose, this STTR fast-track proposal involves several institutions with deep expertise in their respective domains. The proposed Phase I 6-month project is a proof-of- principle demonstration of a breadboard system used on nanophosphors in low oxygen solutions and embedded in bone. The proposed Phase II 24-month project is to develop a complete prototype system and experimentally verify its performance.

项目摘要/摘要众所周知，低氧（低氧）环境对于维持小的氧气很重要人体成年干细胞的数量，例如骨髓中。这些条件是还认为使休眠的癌细胞能够生存并转移数年或几十年后原始肿瘤已被破坏，这是骨髓是最多的原因之一癌症转移的常见部位。了解这些条件可以推动开发用于生长干细胞的3D细胞支架，从而减轻了负担需要骨髓移植，以及开发预防癌症复发的治疗剂。该项目提议开发第一个定量氧层析成像系统称为骨骼（生物组织氧合纳米磷仪的传感）来解决关键需要高分辨率成像缺氧（低氧）组织中的氧气浓度作为骨髓。该技术基于计算的X射线发光中的发展断层扫描，一种能够达到细胞水平的新兴分子成像技术解决方案和高灵敏度。该方法使用X射线激发氧气敏感发射近红外光子的纳米磷剂最终在3D中实现3D氧测量深骨髓。因为该技术需要具有X射线专业知识的多学科团队，所以专业知识，近红外检测专业知识和用于量化浓度的算法并最大程度地减少剂量，此sttr快速提案涉及多个具有深度的机构各自领域的专业知识。拟议的I期6个月项目是一个证明在低氧中使用的纳米磷的面包板系统的原理演示溶液并嵌入骨骼中。拟议的II期24个月项目是开发完整的原型系统并实验验证其性能。