Cell Biology Core

细胞生物学核心

基本信息

批准号：
10404933
负责人：
Matthew J Brady
金额：
$ 30.9万
依托单位：
UNIVERSITY OF CHICAGO
依托单位国家：
美国
项目类别：
财政年份：
1996
资助国家：
美国
起止时间：
1996-12-01 至 2023-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10404933
关键词：
Address Adipocytes Adipose tissue Anabolism Apoptosis Beta Cell Biochemical Biological Biomedical Research Brain Calcium Catabolism Cell Differentiation process Cell Line Cell Proliferation Cell Separation Cell physiology Cells Cellular biology Chicago Circadian Rhythms Collaborations Data Analyses Diabetes Mellitus Disease Embryo Endocrine Ensure Exocytosis Fibroblasts Genetic Genomics Glycogen Goals Health Hepatocyte Human Illinois Image Immune In Vitro Individual Insulin Islet Cell Islets of Langerhans Lipids Lipolysis Liver Metabolic Methods Mission Mus Muscle Cells Pancreas Phosphorylation Physiological Physiology Population Procedures Proinsulin Proteomics Research Research Design Research Personnel Research Training Role Services Source Students System T-Lymphocyte Techniques Technology Testing Time Tissues Training Training and Education Transfection Universities Vesicle base biophysical techniques blood glucose regulation cell type circadian biology diabetic diabetic patient experimental study glucose metabolism glucose production glucose transport improved in vivo induced pluripotent stem cell insulin signaling insulinoma investigator training islet macrophage member neutrophil non-diabetic

项目摘要

PROJECT SUMMARY – CELL BIOLOGY CORE Cell Biology has been a component of the DRTC since its inception in 1977. It has taken many forms over the past 40 years, most recently focusing on studies of pancreatic islets and beta cells. However, in the past 18 months and in this application the Cell Biology Core has been broadened to also include studies of adipocytes, hepatocytes, and innate and adaptive immune cells from normal and diabetic humans and mice. The Core provides a broad range of services in cell-based systems to understand the roles of islet endocrine cells, adipocytes, hepatocytes and immune cells in health and disease with an emphasis of studies using primary cells from humans and mice. The Core provides advice on study design, methods and data interpretation. Since many of the techniques are new to investigators, it also provides direct hands-on support and training to ensure the successful and timely completion of experiments. This Core is centered at the University of Chicago and the University of Illinois at Chicago. The two groups meet regularly and have developed standard operating periods to ensure that Users receive quality support for their studies. The Core also has an ongoing commitment to training and has trained investigators, fellows, students and research technicians in the procedures of the Core. The Cell Biology Core is an integral part of the DRTC, and thus synergizes with the Circadian Biology and Physiology Cores, by facilitating diabetes based research on primary cells and tissues from mice and humans. Moreover, it allows discoveries made in the Genetics and Genomics Core to be tested in a biological context.

项目摘要 – 细胞生物学核心自 1977 年成立以来，细胞生物学一直是 DRTC 的组成部分。它有多种形式过去 40 年来，最近的研究重点是胰岛和 β 细胞。在过去 18 个月中，在本应用中，细胞生物学核心已扩展至还包括对正常和糖尿病患者的脂肪细胞、肝细胞以及先天性和适应性免疫细胞的研究 Core 在基于细胞的系统中提供广泛的服务来理解。胰岛内分泌细胞、脂肪细胞、肝细胞和免疫细胞在健康和疾病中的作用使用人类和小鼠原代细胞进行的研究的重点提供了研究建议。由于许多技术对于研究人员来说都是新的，因此它也是新的。提供直接的实践支持和培训，以确保成功及时地完成该核心实验以芝加哥大学和伊利诺伊大学芝加哥分校为中心。两个小组定期会面并制定了标准操作期限，以确保用户获得高质量的学习支持核心还持续致力于培训并拥有对研究人员、研究员、学生和研究技术人员进行了核心程序的培训。 Biology Core 是 DRTC 的一个组成部分，因此与昼夜节律生物学和生理学核心，通过促进对小鼠原代细胞和组织的糖尿病研究此外，它还允许在遗传学和基因组学核心中进行的发现得到测试。生物学背景。