University of Washington Arboviral Research Network (UWARN)

华盛顿大学虫媒病毒研究网络 (UWARN)

基本信息

批准号：
10399583
负责人：
Michael Gale
金额：
$ 169.2万
依托单位：
UNIVERSITY OF WASHINGTON
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-05-22 至 2025-04-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10399583
关键词：
Acute Address Administrative Coordination Alphavirus Animals Antibodies Arbovirus Infections Arboviruses Area Arthropod Vectors Back Bacteriology Binding Biological Assay Biological Markers Blood Body Fluids Brazil Cell Separation Chikungunya virus Clinical Clinical Management Clinical Trials Collaborations Collection Congo Containment Data Dengue Dengue Infection Dengue Virus Detection Development Diagnosis Diagnostic Diagnostic Reagent Disease Emergency Situation Emerging Communicable Diseases Engineering Enzyme-Linked Immunosorbent Assay Epitopes Far East Fever Flavivirus Fluorescence Freezing Future Genomic approach Human Immune response Immune system Immunoglobulin G Individual Infection Infectious Diseases Research Innate Immune Response Innate Immune System Institutes Institution International Intervention Investigation Japanese Encephalitis Knowledge Laboratories Laboratory Research Lead Light Logistics Measurement Metagenomics Microcephaly Molecular Monitor Monoclonal Antibodies Natural Immunity Oropouche virus Orthobunyavirus Outcome Pakistan Parasitic infection Pathogenesis Pathway interactions Patients Population Prevalence Process Production Protein Engineering Proteins Readiness Reagent Research Resources Rift Valley Fever Risk Sampling Senegal Sensitivity and Specificity Serology Seroprevalences Site South Africa South America Specimen Statistical Data Interpretation System Taiwan Technology Technology Transfer Testing The science of Mycology Therapeutic Time Universities Vaccines Variant Veterinarians Viral Viral Antibodies Viral Hemorrhagic Fevers Viral Load result Virus Virus Diseases Washington Yellow Fever ZIKA Zika Virus antagonist arthropod-borne base biobank candidate marker chikungunya cohort cross reactivity data management design experience global health global health emergency human monoclonal antibodies improved improved outcome innovation neutralizing antibody neutralizing monoclonal antibodies next generation sequencing novel novel diagnostics novel strategies pandemic disease pandemic preparedness pathogen pathogenic virus point of care population based prospective public health relevance severe dengue therapeutic target transcriptomics viral detection virology virus host interaction

项目摘要

Abstract: The United World Antiviral Research Network (UWARN, formerly referred to as the University of Washington Arboviral Research Network) will address emerging and re-emerging infectious diseases by carrying out research on arboviruses that include current high burden pathogens including dengue, chikungunya, and Zika viruses. Arboviruses also include emerging and re-emerging viruses such as Mayaro, Una, Usutu, Japanese encephalitis, yellow fever, Crimean-Congo hemorrhagic fever, and Oropouche viruses. UWARN will leverage the UW Alliance (formerly Metacenter) for Pandemic Disease Preparedness and strong research laboratory partners in Brazil, Senegal, South Africa, Pakistan and Taiwan as well as the UW Department of Global Health global reach in capacity building. The UWARN international Collaborating Partners have institution-based and population-based cohorts in place to carry out the planned research and monitor for new virus emergence. The proposed UWARN research will create new human viral-neutralizing monoclonal antibodies (Hu-nMabs) that can be used as therapeutics or diagnostics. UWARN research will also create new diagnostics, created ab initio by the Institute for Protein Design, as artificial proteins that release light when antibodies to virus are present in body fluids. Finally, UWARN research will generate an understanding of how viruses manipulate the human innate immune system, and this information will be used to design biomarkers to predict severe disease as well as to suggest host-directed therapies that could lead to better outcomes after arboviral infection. The proposed technologies are generic and could be rapidly adapted to any future emerging arbovirus threat. UWARN collaborators span virology, bacteriology, mycology, and parasitic infections, such that UWARN can respond to diverse emerging infectious diseases threats. UWARN international laboratories have advanced capabilities including biorepositories with generator-power backed-up -80C freezers, next-generation sequencing (NGS) capabilities, fluorescence cell sorting, and BSL-3 containment facilities. UWARN cohorts have biobanked samples taken from individuals during acute febrile illnesses, with known and unknown arboviral diseases, and partnership sites are poised for prospective collection of population and facility–based samples. UWARN labs will use metagenomic NGS to detect novel viral emergence in affiliated cohorts. UWARN Partners have collaborating entomologists and veterinarians to sample arthropod vectors and animal reservoirs as needed. Additional innovative capacity included the Brazil UWARN partner’s mobile van with on board NGS capability for real-time, on-site viral sequencing and sampling to viral identification in less than 18 hrs. A second partner, IRESSEF in Senegal, plans to set up a similar mobile vehicle for viral sampling and sequencing in Senegal and the Brazil group will collaborate with IRESSEF on technology transfer and best practices. UWARN partners will serve as a resource to other Emerging Infectious Diseases Research Centers (EIDRC) providing expertise in monoclonal antibodies, protein design, and host directed therapies.

摘要：联合世界抗病毒研究网络（以前称为华盛顿大学的Arbovialal Research Network）ll解决了有关Arbovovirusses on Arbovovirusses Engue，Chikungunya和Re-Re-Re-and Arso的新兴和重新散布。诸如Mayaro，UNA，USUTU，日本脑炎ean-congo gongo gongo gongo gongo gongo gongo gongo gongo gongo gongo gongo gongo gongo gongo gongo gongo gongo gongo gongo gongo gongo gongo gongo gongo gongo gongo gongo gongo gongo gongo gongo gongo gongo gongo gongo gongotha和opouche病毒国际合作合作伙伴拥有基于Intictition的同类群体，可以进行计划的研究并监测新的病毒出现，并使用创建新的人类病毒性中和单网元（Hu-nmabs）的uwarn研究。或诊断。一般而言，可以适应未来新兴的arbovirus感染。 C. SES和伙伴关系的伙伴关系是人口的，并在附加的伙伴中探测了额外的风险。在不到18个小时的情况下，将病毒和对其他感染性疾病研究中心（EIDRC）进行配合。