Dopamine system as reporter of HIV status and inflammation in Meth abusers

多巴胺系统作为冰毒滥用者艾滋病毒状况和炎症的报告者

基本信息

批准号：
10398692
负责人：
Maria Cecilia Garibaldi Marcondes
金额：
$ 0.63万
依托单位：
SAN DIEGO BIOMEDICAL RESEARCH INSTITUTE
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-02-01 至 2024-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10398692
关键词：
Affinity Age Agonist Behavior Big Data Biological Process Blood Cells Blood donor Brain CCR5 gene Cell surface Cells Cellular Indexing of Transcriptomes and Epitopes by Sequencing Central Nervous System Diseases Cognitive Computer software Critical Thinking DNA DRD1 gene DRD4 gene Data Set Dopamine Dopamine Receptor Drug Exposure Environment Exercise Frequencies Genes Genetic Genetic Polymorphism Genetic Transcription Genomics Genotype HIV HIV Infections HIV Receptors Human IL6 gene Immune Immune Targeting Immune response Immune system In Vitro Individual Individual Differences Infection Inflammation Inflammatory Intake Interleukin-10 Internships Learning Leukocytes Life Expectancy Link METH abuser Mathematics Measures Methamphetamine Methods Microglia Modeling Molecular National Institute of Drug Abuse National NeuroAids Tissue Consortium Neuraxis Neuroimmune Neurologic Neurological outcome Neurotransmitters Opioid Organ Outcome Pathogenesis Pattern Performance Peripheral Peripheral Blood Mononuclear Cell Pharmaceutical Preparations Plasma Predisposition Protein Isoforms RARG gene RNA Reporter Rewards Risk Role S100A8 gene Sequence Alignment Signal Transduction Single Nucleotide Polymorphism Specimen Statistical Data Interpretation Statistical Models Students System Systems Biology Testing Training Viral Viral reservoir Virus Virus Diseases Virus Latency cell type chronic infection co-infection cognitive performance comorbidity cytokine dopamine system dopamine transporter drug abuser experimental study inflammatory marker inflammatory milieu innate immune function insight large datasets macrophage methamphetamine use methamphetamine user neuroAIDS neuroinflammation neuropathology peripheral blood polysubstance use programs receptor social substance use substance user summer internship summer research tool viral DNA viral RNA virology virtual

项目摘要

2021 NIDA Summer Research Internship Program Systems Biology Approaches to identify Biological Processes associated with Genomic effects of Dopamine in HIV-infected Macrophages Abstract Macrophages, microglia and other immune cells that are targets of Human Immunodeficiency Virus infection express receptors to dopamine, a neurotransmitter that is highly increased in the brain of stimulant drug abusers, including Methamphetamine. Our lab has performed single cell CITE-seq experiments to determine the role of dopamine in reactivating on maintaining the viral reservoir. The Summer Internship student will learn to analyze large sets of data to produce integrated models of biomolecular networks. The student will be virtually trained to administer systems biology strategies to big data and learn state-of-the-art methods for predicting gene networks expressing synchronized behaviors. At the end of the internship, the student will master a systems biology software, while exercising critical thinking, and produce results from our data set.

2021年NIDA暑期研究实习计划系统生物学方法来识别与基因组效应相关的生物过程 HIV感染巨噬细胞中的多巴胺抽象的巨噬细胞、小胶质细胞和其他免疫细胞是人类免疫缺陷病毒感染的目标表达多巴胺受体，多巴胺是一种神经递质，在兴奋剂药物的作用下大脑中会大大增加滥用者，包括甲基苯丙胺。我们的实验室进行了单细胞 CITE-seq 实验以确定多巴胺在重新激活维持病毒库方面的作用。暑期实习生将学习分析大量数据以生成生物分子网络的集成模型。该学生将是经过虚拟培训，可以管理大数据的系统生物学策略并学习最先进的方法预测表达同步行为的基因网络。实习结束时，学生将掌握系统生物学软件，同时锻炼批判性思维，并从我们的数据集中产生结果。