Understanding NLRP6 function in intestinal homeostasis

了解 NLRP6 在肠道稳态中的功能

基本信息

批准号：
10393573
负责人：
GRACE Y. CHEN
金额：
$ 40.02万
依托单位：
UNIVERSITY OF MICHIGAN AT ANN ARBOR
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-07-01 至 2023-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10393573
关键词：
Acute Affect Bacteria CASP1 gene Caspase Cells Chronic Citrobacter rodentium Colitis Colon Colon Carcinoma Complex Data Development Epithelial Family member Goals Hematopoietic Homeostasis Host Defense Impairment Infection Inflammasome Inflammation Inflammatory Inflammatory Bowel Diseases Inflammatory Response Interferons Interleukin-10 Interleukin-18 Intestines Ligands Maintenance Mediating Microbe Mucous body substance Mus Myeloid Cells Pathogenesis Pathology Pathway interactions Pattern recognition receptor Phenotype Play Predisposition Production Publishing Regulation Reporting Resistance Resolution Role Signal Pathway Signal Transduction Structure Time Tissues Wild Type Mouse antimicrobial antimicrobial peptide chemically induced colitis chemokine chronic inflammatory disease colon tumorigenesis commensal bacteria cytokine dysbiosis enteric virus infection epithelial injury germ free condition gut homeostasis gut inflammation gut microbiome gut microbiota intestinal epithelium intestinal homeostasis macrophage microbial microbial community microbiome alteration mortality mouse model neutrophil pathogen pathogenic bacteria protective effect receptor repaired tissue injury treatment strategy tumor

项目摘要

PROJECT SUMMARY Nod-like receptors (NLRs) are intracellular pattern-recognition receptors that are involved in host defense, but have emerged as critical players in the maintenance of tissue homeostasis, especially within the intestine. Capable of sensing microbial products as well as endogenous ligands related to tissue injury, NLRs activate multiple downstream inflammatory signaling pathways, including the inflammasome, to promote the production of cytokines and chemokines important for pathogen clearance and the timely repair of intestinal epithelial damage. There is also increasing evidence that NLRs participate in the regulation of the gut microbiome. Consequently, mice that are deficient in certain NLRs have altered microbiomes associated with increased susceptibility to colitis and colon tumorigenesis. In particular, mice deficient in NLRP6 develop more severe inflammation and tumors compared to wildtype mice in mouse models of inflammatory bowel disease and inflammation-associated colon cancer. This phenotype is associated with impaired IL-18 production and an altered microbiome. However, the cellular mechanisms by which NLRP6 mediate its protective effects within the colon is unclear. NLRP6 is highly expressed in the epithelium and consistently, there have been multiple reports that demonstrate epithelial-specific roles for NLRP6, including the production of IL-18, antimicrobial peptides, and mucus. However, NLRP6 expression can also be induced in hematopoietic cells, and our published data suggests important roles for NLRP6 in the regulation of IL-18 and intestinal inflammatory responses in hematopoietic cells. Thus, the mechanism by which NLRP6 protects against colitis is likely to be complex. The goal of the current proposal is to further delineate NLRP6 function in myeloid cells and the inflammatory signaling pathways NLRP6 engages in to affect resistance to intestinal pathology.

项目摘要 NOD样受体（NLR）是细胞内模式识别受体，与宿主有关防御，但已经成为维持组织体内平衡的关键参与者，特别是在肠内。能够感测微生物产物以及内源性与组织损伤有关的配体，NLR激活多个下游炎症信号传导包括炎症体在内的途径，以促进细胞因子和趋化因子的产生对于病原体清除和及时修复肠上皮损伤的重要性。有越来越多的证据表明NLR参与了肠道微生物组的调节。因此，在某些NLR中不足的小鼠已经改变了与增加了对结肠炎和结肠肿瘤发生的敏感性。特别是，NLRP6缺乏的小鼠与野生型小鼠相比，与野生型小鼠相比炎症性肠病和与炎症相关的结肠癌。这种表型是与IL-18产生受损和微生物组改变有关。但是，细胞 NLRP6在结肠内介导其保护作用的机制尚不清楚。 NLRP6 在上皮中高度表达，并且始终存在多个报告演示NLRP6的上皮特异性作用，包括产生IL-18，抗菌剂肽和粘液。但是，NLRP6表达也可以在造血细胞中诱导，并且我们已发布的数据提出了NLRP6在IL-18和肠道调节中的重要作用造血细胞中的炎症反应。因此，NLRP6保护的机制反对结肠炎可能很复杂。当前建议的目的是进一步描述 NLRP6在髓样细胞中的功能和炎症信号通路NLRP6参与影响对肠道病理的抗药性。