The Exercise and Physical Activity Collaborative Team (ExPACT): a Proposed MoTrPAC Clinical Center

锻炼和身体活动协作团队 (ExPACT)：拟建 MoTrPAC 临床中心

• 批准号: 10391628
• 负责人: MARCAS M BAMMAN
• 金额: $ 9.35万
• 依托单位: BALL STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-12-06 至 2022-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10391628
• 关键词: 18 year old; Acute; Adipocytes; Adipose tissue; Aerobic; Aerobic Exercise; Alabama; Ancillary Study; Bioinformatics; Biological; Biology; Blood; Cellular Assay; Chemicals; Chronic; Clinical; Clinical Trials; Collaborations; Collection; Complex; Coupled; Data; Deposition; Development; Diabetes Mellitus; Disease; Enrollment; Ensure; Exercise; Florida; Funding; Future; Genetic; Geography; Goals; Health; Health Benefit; Heterogeneity; Human; In Vitro; Information Dissemination; Intervention Trial; Investigation; Knowledge; Laboratories; Leadership; Link; Manuscripts; Maps; Medicine; Metabolic; Metabolism; Methodology; Mitochondria; Molecular; Muscle; Muscle Fibers; Myoblasts; Outcome; Performance; Phase; Phenotype; Physical activity; Physiological; Pilot Projects; Population; Positioning Attribute; Procedures; Protocols documentation; Public Health; Quality Control; Ramp; Randomized Clinical Trials; Recording of previous events; Research Institute; Resistance; Resources; Rural; Sister; Site; Standardization; Stimulus; Structure; Testing; Time; Tissues; Training; Transducers; Translational Research; United States National Institutes of Health; Universities; Woman; Work; Writing; circadian; clinical center; design; ethnic diversity; exercise training; experience; fitness; human tissue; improved; innovation; men; metabolic abnormality assessment; preservation; programs; racial and ethnic; randomized trial; recruit; resistance exercise; response; sedentary; suburb; success

The Molecular Transducers of Physical Activity Consortium (MoTrPAC) is designed to discover and characterize the range of molecular transducers (the `molecular map') that underlie the effects of physical activity (PA) in humans. We formed an interdisciplinary team with the requisite experiences to contribute significantly to the overall success of MoTrPAC. Our Exercise and Physical Activity Collaborative Team (ExPACT) – an alliance of the University of Alabama at Birmingham Center for Exercise Medicine (UAB), Translational Research Institute for Metabolism and Diabetes in Orlando, Florida (TRI-MD), and Ball State University Human Performance Laboratory (BSU) – has extensive experience performing highly-controlled aerobic (AT) and resistance (RT) exercise training clinical trials in healthy and diseased populations. We have a lengthy history of maximizing human biospecimens (muscle, adipose, blood) to reveal molecular underpinnings linked to physiological and clinical outcomes of acute and chronic exercise stimuli. Moreover, the overarching goal of ExPACT is to contribute substantively to successful MoTrPAC planning, execution, and innovation. Phase I (Planning Year) Aim 1. We will effectively collaborate with MoTrPAC leaders and NIH leadership to help guide and finalize the structure and standard operating procedures (SOPs). Aim 2. Leveraging institutional resources committed to ExPACT and our previous experience, we propose to collect pilot biospecimens to facilitate the MoTrPAC Chemical Analysis Sites' ability to optimize analyses. Phase II (Recruitment and Testing Years 2-5) Aim 1. We will enroll 420 sedentary but healthy men and women 18+ y into a rigorously controlled, 12-wk, randomized trial of AT (n=185), RT (n=185), or no-exercise control (CON, n=50). ExPACT geography will enrich MoTrPAC racial, ethnic, urban, suburban, and rural diversity. The design incorporates comprehensive phenotyping to assess fitness and clinical outcomes, and human tissue phenotyping to link the molecular transducers with key biological responses to exercise. Aim 2. Pre- and post- training, we will obtain strategically timed biospecimens before and after acute exercise. To better understand the biological extremes, we will conduct the same acute response studies in highly-trained athletes (AT (n=20) or RT (n=20)). Serial biospecimen collection in CON will allow estimation of biological, seasonal, circadian and methodological variability. Aim 3. To enhance the overall scientific impact of MoTrPAC, we will seek additional funding to conduct innovative ancillary studies, which will markedly augment subject and tissue phenotyping and interpretation of the molecular maps. Phase III (Close-out Year 6) Aim 1. We will complete all ExPACT data entry, quality control, and central deposit procedures in collaboration with the MoTrPAC Coordinating Center, and we will actively contribute to the completion of biospecimen analyses, manuscript writing, and dissemination of results. We would be honored to collaborate with MoTrPAC to contribute future knowledge on the molecular mechanisms through which physical activity can improve or preserve health.

体育活动联盟（MOTRPAC）的分子换能器旨在发现和 表征分子传感器（“分子图”）的范围 人类的活动（PA）。我们组建了一个跨学科团队，并拥有必要的经验来贡献 显着取得MOTRPAC的整体成功。我们的运动和体育锻炼协作团队 （外籍） - 伯明翰运动医学中心（UAB）的阿拉巴马大学联盟， 佛罗里达州奥兰多的代谢和糖尿病转化研究所（TRI-MD）和球状态 大学人类绩效实验室（BSU） - 具有高度控制的丰富经验 有氧和耐药性（RT）运动训练临床试验在健康和失常的人群中进行临床试验。我们有 最大化人类生物测量（肌肉，脂肪，血液）的冗长历史，以揭示分子 与急性和慢性运动刺激的物理和临床结果有关的基础。而且， 外籍的总体目标是为成功的Motrpac计划，执行， 和创新。第一阶段（计划年）目标1。我们将有效与Motrpac领导者和NIH合作 帮助指导和确定结构和标准操作程序（SOP）的领导力。目标2。 利用致力于剥离和我们以前的经验的机构资源，我们建议收集 驾驶生物测量促进Motrpac化学分析位点优化分析的能力。第二阶段 （招聘和测试2-5年）AIM 1。我们将注册420个久坐但健康的男性和女性18岁以上 进行严格控制的12周，随机试验（n = 185），RT（n = 185）或无运动控制（con，con， n = 50）。外界地理将丰富Motrpac种族，种族，城市，郊区和粗糙的多样性。设计 结合全面的表型，以评估适应性和临床结果和人体组织 表型将分子传感器与对运动的关键生物学反应联系起来。目标2。前后 培训，我们将在急性运动之前和之后获得战略性定时的生物测量。更好地理解 生物学极端，我们将在训练有素的运动员中进行相同的急性反应研究（at（n = 20） 或RT（n = 20））。连续的生物传播收集将允许估计生物学，季节性，昼夜节律 方法上的变异性。目标3。为了增强Motrpac的整体科学影响，我们将寻求更多 进行创新辅助研究的资金，该研究将显着增加主题和组织表型 和分子图的解释。第三阶段（闭幕式6年）目标1。我们将完成所有展览 与MOTRPAC协调合作的数据输入，质量控制和中央存款程序 中心，我们将积极为完成生物测量分析，手稿写作和 结果传播。我们很荣幸与Motrpac合作，以贡献有关未来的知识 体育活动可以改善或保留健康的分子机制。