Mechanism of Microbial DNA Hypervariation through Mutagenic Transposition

通过诱变转座导致微生物 DNA 高度变异的机制

基本信息

批准号：
10387714
负责人：
Donald H Burke
金额：
$ 9.21万
依托单位：
UNIVERSITY OF MISSOURI-COLUMBIA
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-09-19 至 2023-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10387714
关键词：
Address Adenine Amino Acid Substitution Archaea Bacteria Bacteriophages Base Pairing Biochemical Bioinformatics Biomedical Engineering Bordetella Chemicals Complementary DNA Complex DNA Detection Development Dyes Elements Environment Family Funding Genes Genetic Genetic Polymorphism Homing Human Hybrids Integration Host Factors Investigation Label Light Liquid substance Mediating Metagenomics Methodology Molecular Mutagenesis Mutation Nucleotides Oligonucleotides Paper Performance Phylogenetic Analysis Play Preparation Process Property Proteins RNA RNA Sequences RNA primers RNA-Directed DNA Polymerase Reaction Request for Proposals Research Retroelements Reverse Transcription Ribonucleoproteins Role Site Structure Tropism Variant Virus Work in vivo instrument instrumentation laboratory equipment macromolecule medical specialties microbial microorganism nucleic acid binding protein nucleotide analog parent grant receptor small molecule

项目摘要

This proposal requests funds for a high-performance liquid chromatograph (HPLC) that is suitable for both analytical and preparative work, as a major instrumentation supplement in support of the parent grant R01GM125669, Mechanism of Microbial DNA Hypervariation through Mutagenic Transposition. A highcaliber HPLC is a critical piece of standard laboratory equipment for almost any cutting edge biochemical research. This instrument will be used in preparative capacity for purification of chemically-modified oligonucleotides (such as dye-labeled primers and site-specifically modified DGR cDNA intermediates), proteins (such as bRT and bAvd from Bordatella phage and from phylogenetically related phage), and specialty small molecules (such as modified nucleotide analogs). We would also use the instrument in analytical capacity for quantitative assessment of chemical purity of reaction products involving small molecules, macromolecules, and conjugated variants of the macromolecules.

该提案要求资金用于适合两者的高性能液相色谱仪（HPLC）分析和准备工作，作为支持父母赠款的主要仪器补充 R01GM125669，通过诱变转座的微生物DNA过变量的机理。 Highcaliber HPLC是几乎所有最先进生化的标准实验室设备的关键部分研究。该仪器将以制备能力用于化学修饰的纯化能力寡核苷酸（例如染料标记的底漆和特定于位置修饰的DGR cDNA 中间体），蛋白质（例如Bordatella Phage的BRT和BAVD，以及从系统发育相关的噬菌体）和专业小分子（例如修饰的核苷酸类似物）。我们还将使用具有分析能力的仪器，以定量评估反应产物的化学纯度涉及大分子，大分子和大分子的共轭变体。