DanFreez: Zebrafish Genetically-Optimized for Cryogenic Storage of Embryos

DanFreez：针对胚胎低温储存进行基因优化的斑马鱼

基本信息

批准号：
10385461
负责人：
Trisha Brock
金额：
$ 46.47万
依托单位：
NEMAMETRIX, INC.
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-08-15 至 2024-08-14
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10385461
关键词：
AFP gene Achievement Acute Adopted Adult Aftercare Animal Model Animals Biological Biological Assay Biomedical Research Caenorhabditis elegans Carrier Proteins Catalogs Chemicals Clustered Regularly Interspaced Short Palindromic Repeats Code Communities Cryopreservation Databases Development Diapause Disease Disease model Ectopic Expression Embryo Embryonic Development Engineering Enhancers Environmental Risk Factor Enzyme-Linked Immunosorbent Assay Evaluation Freezing Funding Genes Government Grant Growth Hour Killifishes Messenger RNA Metabolic Methods Modeling Molecular Molecular Chaperones Monitor Morphology Mus Names Nuclear Organism Persons Phase Proteins Protocols documentation Reagent Receptor Signaling Records Research Research Personnel Resources Rodent Model Secure Services Small Business Innovation Research Grant Structure Survival Rate Suspensions Systems Development Techniques Technology Testing Transgenic Organisms Trehalose United States National Institutes of Health Vitamin D3 Receptor Western Blotting Work Zebrafish antagonist biobank cryogenics hormonal signals mRNA capping preservation protein expression sperm cell success survivorship tool trend

项目摘要

Summary: Unlike C. elegans and mice, methods for cold storage of zebraﬁsh lines are lacking in the marketplace. We propose the use of environmental and gene c factors to enable cold storage of viable embryos at frozen or near freezing condi ons. In Aim 1 we will explore the use of transient protein expression to produce proteins associated with anhydrobio c status. In Aim 2 we explore the induc on of genes involved in diapause in killiﬁsh as a mechanism to promote cold-storage survival. In Aim 3, we will look at combina ons of gene c and environmental factors that promote reanima on of zebraﬁsh embryos from cold storage. Achievement of any one of these aims that result in high viability yields from cold storage can then be developed in Phase II as a gene cally integrated line, which can then be broadly commercialized as a product or service to aid researchers in their biobanking eﬀorts with their zebraﬁsh lines.

概括：与线虫和小鼠不同，市场上缺乏斑马鱼系的冷藏方法。利用环境和基因 c 因素在冷冻或接近冷冻条件下冷藏可存活的胚胎。在目标 1 中，我们将探索使用瞬时蛋白表达来产生具有脱水状态的相关蛋白。在目标 2 中，我们探索了鳉鱼滞育相关基因的诱导，作为促进冷藏的机制。在目标 3 中，我们将研究基因 c 和促进复活的环境因素的组合。冷藏中的斑马鱼胚胎实现这些目标中的任何一个，从而通过冷藏获得高存活率。然后可以在第二阶段开发存储作为基因整合线，然后可以作为帮助研究人员利用斑马鱼品系进行生物样本库工作的产品或服务。