OpenScope: A Platform for High-Throughput and Reproducible Neurophysiology Open to External Scientists to Test Impactful Theories of Brain Function

OpenScope：向外部科学家开放的高通量和可重复的神经生理学平台，用于测试有影响力的大脑功能理论

• 批准号: 10379878
• 负责人: CHRISTOF KOCH
• 金额: $ 149.42万
• 依托单位: ALLEN INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-04-01 至 2026-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10379878
• 关键词: Address; Anatomy; Animal Experiments; Animals; Area; Astronomy; BRAIN initiative; Back; Behavioral; Biomedical Research; Blinded; Brain; Calcium; Cells; Code; Communities; Complex; Core Facility; Data; Data Analyses; Data Collection; Data Set; Detection; Disease; Educational workshop; Electrophysiology (science); Engineering; Environment; Fostering; Funding; Future; Glass; Hawaii; Health; Image; Incentives; Individual; Institutes; Institution; Knowledge; Laboratories; Location; Machine Learning; Maps; Modeling; Modernization; Mus; Neurons; Neurosciences; Operative Surgical Procedures; Optics; Physiology; Procedures; Process; Protocols documentation; Publications; Quality Control; Reproducibility; Reproducibility of Results; Research; Research Personnel; Resources; Running; Scientist; Silicon; Site; Standardization; Structure; Surveys; System; Talents; Technical Expertise; Techniques; Technology; Testing; Thalamic structure; Training; Variant; Visual; base; brain research; cell type; cloud based; data acquisition; data format; data standards; design; experimental study; falls; imaging platform; implantation; in vivo; innovation; instrument; neural circuit; neurophysiology; novel; particle physics; programs; relating to nervous system; software development; statistics; success; theories; tool; two-photon; visual stimulus; web portal

Project summary Over the past five years, the Allen Institute has built two unique in-house pipelines for in vivo electro- and optical-physiology: together they form the Allen Brain Observatory. We used this observatory to freely and openly share calcium imaging data from ~60,000 cells from 221 running mice viewing standard visual stimuli; in October 2019, we will release Neuropixels electrophysiology recordings from ~100,000 cells from 100 mice under the same conditions, all registered to a common anatomical coordinate system (CCFv3). We wish to open the Brain Observatory pipeline to the community, enabling theoretical, computational, and experimental scientists to test sophisticated hypotheses on brain functions in a process analogous to astronomical observatories that survey the night sky. Our observatory, called OpenScope, will accept, once a year, experimental proposals from external scientists, which will be reviewed by a panel of leading experts for their feasibility and scientific merit. The Allen Institute will carry out the selected experiments by following verified, reproducible, and open protocols for in vivo single- and multi-area two photon calcium imaging and Neuropixels electrophysiology, making the data freely available to these scientists. Funding for this proposal will give the entire community access to a modern experimental platform that operates under standardized, reproducible, and high-throughput conditions. This resource will be particularly attractive to the computational and theoretical communities with limited access to experimental facilities. Our project has the potential to transform systems neuroscience by implementing an observatory model that has been tremendously successful in other scientific fields. By reducing variability in training, imaging and quality control across animals and experiments and by lowering the barrier to select, test, and validate the best ideas, we hope to accelerate progress toward an integrated view of neural circuits.

项目摘要 在过去的五年中，艾伦学院（Allen Institute 光学生理学：它们共同形成了艾伦脑观测站。我们将此天文台自由使用， 从221个运行的小鼠观察标准视觉刺激的221个细胞中公开共享约60,000个细胞的钙成像数据； 2019年10月，我们将从约100,000个细胞中释放100,000个细胞的神经偶像电池生理记录 在相同的条件下，所有均注册为通用解剖坐标系（CCFV3）。我们希望 向社区打开大脑天文台管道，实现理论，计算和实验性 科学家在类似于天文学的过程中测试有关大脑功能的复杂假设 调查夜空的观察者。我们的天文台称为OpenScope，每年将接受一次 外部科学家的实验建议，将由一群领先的专家小组审查 可行性和科学功绩。艾伦学院将通过以下验证，进行选定的实验， 可重现的体内单区和多面积两个光子钙成像和开放方案 Neuropixels电生理学，使这些科学家可以自由使用。该提议的资金 将使整个社区都可以访问一个现代实验平台，该平台在标准化下运行， 可再现和高通量条件。该资源对计算特别有吸引力 以及理论上的社区，获得实验设施的机会有限。我们的项目有可能 通过实施极大的天文台模型来改变系统神经科学 在其他科学领域取得了成功。通过降低培训，成像和质量控制的变异性 动物和实验以及降低选择，测试和验证最佳想法的障碍，我们希望 加速进步，朝着神经回路的综合视图。