Developing plant synthetic biology platforms to elucidate the role of natural products

开发植物合成生物学平台以阐明天然产物的作用

• 批准号: 10464431
• 负责人: Patrick Shih
• 金额: $ 22.04万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-08-01 至 2022-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10464431
• 关键词: Developing; plant; synthetic; biology; platforms

Project Summary and Abstract No change to the Project Summary and Abstrasct will be made with the transfer of this award from UC Davis to UC Berkeley. The change in recipient institution is not related to concerns about safety and/or work environments (e.g. due to concerns about harassment, bullying, retaliation, or hostile working conditions) involving the PD/PI. Plants produce a wealth of natural products that have wide-ranging effects on human nutrition, disease, and overall wellbeing. However, because of the complexities of many of these specialized metabolites, we have been limited in our ability to study the effects of individual phytochemicals on human health. Recently, the nascent field of synthetic biology has provided the means to dissect biological systems into their individual components, enabling scientists to reverse engineer and reconstruct their biochemical makeup. This approach has largely been limited to simple organisms (e.g., E. coli and yeast); however, plants provide a unique platform to leverage synthetic biology. My research focuses on introducing design and engineering principles to rationally manipulate plant metabolism in order to investigate the biosynthesis and physiological roles of plant natural products. Edible cruciferous plants (e.g., broccoli, bok choy) have been implicated in cancer prevention, stemming from their diversity of indole glucosinolate derivatives. However, because of the vast diversity of bioactive compounds produced in cruciferous plants, it is challenging to tease apart and pinpoint the specific molecules that may be responsible for a trait as complex as cancer prevention. As a result, many studies have resulted in conflicting findings and tenuous links between glucosinolates and their claimed nutritional benefits. Engineering specific target molecules into novel hosts with no basal biological activity may provide insight and help clarify their role in human health at a molecular level. The development of plant synthetic biology platforms to produce and deliver specific concentrations of target plant natural products will enable future studies to more quantitatively study the claimed benefits and effects of glucosinolates on human health.

项目摘要和摘要 通过将该奖项从加州大学戴维斯（UC Davis）转移到UC 伯克利。接收者机构的变化与对安全和/或工作环境的担忧无关（例如 涉及PD/PI的骚扰，欺凌，报复或敌对工作条件的担忧。 植物生产大量天然产品，对人类营养，疾病和整体产生广泛影响 福利。但是，由于许多这些专业代谢产物的复杂性，我们在我们的 能够研究单个植物化学物质对人类健康的影响。最近，合成生物学的新生领域 已经提供了将生物系统剖析到其各个组件中的方法，使科学家能够逆转 工程和重建其生化构成。这种方法在很大程度上仅限于简单的生物（例如，E。 大肠杆菌和酵母）；但是，植物提供了一个独特的平台来利用合成生物学。我的研究重点 引入设计和工程原则以合理操纵植物代谢，以调查 植物天然产物的生物合成和生理作用。可食用的十字花科植物（例如西兰花，Bok Choy）具有 与预防癌症相关，是由于它们的吲哚葡萄糖苷衍生物的多样性。然而， 由于十字花科植物中产生的生物活性化合物的大量多样性，取笑和 查明可能导致与预防癌症一样复杂的特定分子。结果，许多 研究导致发现矛盾的发现和葡萄糖醇之间的微弱联系及其所声称的营养 好处。没有基础生物学活性的新型宿主的工程特定靶标分子可能会提供洞察力和 帮助阐明他们在分子水平上在人类健康中的作用。植物合成生物学平台的发展 生产并提供特定浓度的目标植物天然产品将使以后的研究能够增加 定量研究葡萄糖剂对人类健康的益处和影响。