Role of the METTL13 Lysine Methyltransferase in Signaling and Cancer

METTL13 赖氨酸甲基转移酶在信号传导和癌症中的作用

基本信息

批准号：
10338153
负责人：
Or P. Gozani
金额：
$ 45.94万
依托单位：
STANFORD UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-03-01 至 2024-02-29
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10338153
关键词：
Ablation Attenuated Biochemical CRISPR/Cas technology Cancer Biology Cancer Etiology Cancer cell line Cell Proliferation Cell Survival Cells Cytoplasmic Protein Data Data Set Development Disease Elongation Factor Engineering Enzymes Excision FRAP1 gene Family Foundations Genes Genomics Goals Growth Growth and Development function Guanosine Triphosphate Phosphohydrolases Human In Vitro KRAS oncogenesis KRAS2 gene Knowledge Link Lung Adenocarcinoma Lysine Maintenance Malignant - descriptor Malignant Neoplasms Malignant neoplasm of lung Malignant neoplasm of pancreas Mammalian Cell Messenger RNA Meta-Analysis Methylation Methyltransferase Modeling Modification Molecular Molecular Genetics Mus Neoplasms Orphan PI3K/AKT Pancreatic Ductal Adenocarcinoma Pathologic Pathway interactions Patients Pharmaceutical Preparations Phenotype Physiological Post-Translational Protein Processing Pre-Clinical Model Protein Biosynthesis Protein Kinase Protein Methyltransferases Proteins Proteome Proteomics Regulation Reporting Role Signal Transduction Substrate Specificity System Testing Translations Up-Regulation Work base cancer cell cancer initiation cell growth experimental study human model human tissue in vitro activity in vivo inhibitor mRNA Expression mouse model multidisciplinary novel novel therapeutic intervention overexpression pancreatic cancer model pancreatic ductal adenocarcinoma model patient derived xenograft model pre-clinical synergism therapeutic candidate therapeutic target translational approach tumor tumorigenesis tumorigenic

项目摘要

ABSTRACT Our overarching goal is to better understand the role of post-translational modifications (PTMs) of cellular proteins in cancer with the underlying assumption that the enzymes that catalyze the addition or the removal of these PTMs are candidate therapeutic targets in cancer. While enzymes such as protein kinases have been extensively studied in cancer, new classes of enzymes have recently emerged as potential cancer targets. In particular, more than 100 lysine methyltransferases (KMTs) are predicted to be present in the human proteome and many are implicated in cancer etiology. However, the catalytic activity and substrate specificity for many of these enzymes remains unknown. In particular, functions for the vast majority of the large family of seven β- strand KMTs are not known. A central hypothesis to be tested here is that uncovering the activities of these orphan KMTs may provide new links between protein lysine methylation signaling and cancer biology. Here we focus on the candidate KMT METTL13 (Methyltransferase-like protein 13) as a potential critical regulator of tumorigenesis. The METTL13 gene is amplified in many cancers and METTL13 mRNA is over- expressed in diverse tumor types, including several were METTL13 expression negatively correlates with patient survival. However, METTL13 is an orphan enzyme and knowledge of any potential substrates and the overall mode of action of this protein in cells and in vivo is obscure. Based on preliminary observations, we hypothesize that METTL13 up-regulates protein translation to promote tumorigenesis. In Aim 1 we characterize the physiologic catalytic activity of METTL13 and the molecular functions of METTL13 in the regulation of eEF1A activity in vitro and in protein synthesis in cells. We will also investigate METTL13 and methylated eEF1A interacting partners and how these pathways intersect to influence cancer cell phenotypes. The goal of Aim 2 is to elucidate the role of METTL13 in tumors driven by oncogenic KRAS. We will test the hypothesis that METTL13, via its methylation activity, cooperates with KRAS signaling to promote the unlimited expansion of cancer cells in vivo using mouse models of pancreatic ductal adenocarcinoma and lung adenocarcinoma, in which the RAS pathway is frequently activated. We will also investigate the tumorigenic role of METTL13 in human tissue using patient-derived xenograft (PDX) models. We will assess the effects of METTL13 loss on protein translation in vivo. Finally, we will explore potential synergies of METTL13 ablation in combination with inhibitors of cell survival and growth pathways in pre- clinical models of pancreatic and lung cancers.

抽象的我们的总体目标是更好地了解细胞翻译后修饰（PTM）的作用癌症中的蛋白质具有基本假设，即催化添加或去除的酶这些PTM是癌症的候选治疗靶标。而诸如蛋白激酶之类的酶已经在癌症中广泛研究了新的酶，最近已成为潜在的癌症靶标。在特别是，预计100多种赖氨酸甲基转移酶（KMT）存在于人类蛋白质组中并且许多在癌症病因中暗示。但是，许多许多这些酶仍然未知。特别是，七个β-的绝大多数家族的功能链KMT尚不清楚。这里要检验的中心假设是揭示这些活动的活动孤儿KMT可以在蛋白质赖氨酸甲基化信号传导与癌症生物学之间提供新的联系。在这里，我们关注候选KMT Mettl13（甲基转移酶样蛋白13）作为潜在的关键 Mettl13基因在许多癌症中被扩增，Mettl13 mRNA过度在潜水肿瘤类型中表达，包括几种是mettl13表达与患者生存。但是，mettl13是孤儿酶，并且对任何潜在底物的了解，该蛋白在细胞和体内的总体作用方式是晦涩的。根据初步观察，我们假设METTL13上调蛋白质翻译以促进肿瘤发生。在AIM 1中，我们表征了Mettl13的生理催化活性和 METTL13在体外和细胞中蛋白质合成中的EEF1A活性调节中。我们还将调查 Mettl13和甲基化的EEF1A相互作用伴侣以及这些途径如何影响癌症细胞表型。 AIM 2的目的是阐明METTL13在致癌KRAS驱动的肿瘤中的作用。我们将通过其甲基化活性与KRAS信号合作测试Mettl13的假设使用胰腺导管的小鼠模型促进体内癌细胞的无限扩张腺癌和肺腺癌，其中RAS途径经常被激活。我们也会使用患者衍生的Xenographic（PDX）模型研究METTL13在人体组织中的致瘤作用。我们将评估METTL13损失对体内蛋白质翻译的影响。最后，我们将探索潜力 Mettl13消融的协同作用与细胞存活和生长途径的抑制剂结合胰腺癌和肺癌的临床模型。