Animal Studies Core

动物研究核心

• 批准号: 10321602
• 负责人: DARLEEN A. SANDOVAL
• 金额: $ 15.82万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-12-01 至 2022-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10321602
• 关键词: Acute; Address; Animal Model; Animals; Award; Behavior; Blood Glucose; Body Composition; Complex; Computer software; Conscious; Consultations; Diabetes Mellitus; Disease; Educational process of instructing; Effectiveness; Endocrine; Endocrine System Diseases; Equipment; Exposure to; Fee-for-Service Plans; Feeding Patterns; Genetic; Glucose; Glucose Clamp; Goals; Grant; Individual; Interdisciplinary Study; Knowledge; Laboratories; Light; Measures; Metabolic; Metabolic Diseases; Metabolism; Methods; Michigan; Modeling; Mus; Names; Nervous System control; Neuraxis; Neurons; Obesity; Pharmacology; Phenotype; Physiological; Physiology; Publications; Rattus; Research; Research Personnel; Research Technics; Rewards; Rodent; Rodent Model; Services; Standardization; Stress; Techniques; Technology; Time; Tissues; Training; Training and Education; Update; awake; behavioral phenotyping; behavioral response; blood glucose regulation; cost; cost effective; dietary; education research; effectiveness study; experience; feeding; glucose monitor; improved; innovation; islet; member; metabolic phenotype; neural circuit; new technology; optogenetics; recruit

Project Summary/Abstract - Animal Studies Core As it has for over a decade, the fee-for-service MDRC Animal Studies Core (ASC) (previously, the Animal Phenotyping Core- APC) provides state-of-the art equipment, services, training, and consultation regarding the detailed metabolic phenotyping of mouse and rat models of metabolic disease. T o address the previously unmet needs of MDRC members , the MDRC has invested in new technology and established a host of new services over the past five years . The ASC will continue to support these technologies and services, providing access to crucial equipment, expertise and training to empower specialized studies of rodent models of diabetes and related diseases. The ASC consists of four labs: 1) The Rat Metabolic Phenotyping Lab: includes the assessment of glucose homeostasis, whole animal metabolic assessment, body composition, and other specialized metabolic assessments in rats. 2) The Optogenetics and Behavioral Phenotyping Lab: provides training and access to optogenetic equipment to examine physiologic and behavioral responses to neural circuit manipulation, as well as with equipment to measure relevant behaviors, such as homeostatic and non-homeostatic feeding, activity, reward, and other behaviors that impact and/or are regulated by metabolic parameters in rodents. 3) The Continuous Glucose Monitoring Lab: provides continuous assessment of blood glucose concentrations in conscious, unrestrained rodents by radiotelemetry. This technology minimizes the stress of handling rodents and permits a detailed analysis of glucose fluctuations within normal feeding patterns and across extended time-frames. 4) The Islet Lab: provides islet isolation from mice and rats and ex vivo studies (including perifusion) of islets and other endocrine tissues. The ASC directly supports the goals of the MDRC. The services that the ASC provide are unique and are an important means to study rodent models of diabetes and related diseases, without which crucial aspects of diabetes-related research could not be accomplished. This core provides the necessary i nfrastructure to perform advanced, standardized, metabolic phenotyping of animal models of diabetes and related disorders that arise from genetic, pharmacologic, dietary, or other perturbations. The centralized equipment and services expedites research for many MDRC investigators in a cost-effective manner and provides access to complex metabolic techniques that they may not have otherwise.

项目摘要/摘要 - 动物研究核心 正如十多年来所拥有的，用于服务的MDRC动物研究核心（ASC）（以前是动物 表型核心APC）就有关的设备，服务，培训和咨询提供有关 小鼠的详细代谢表型和代谢疾病的大鼠模型。 t o解决以前的 MDRC成员的需求未满足 ，MDRC已投资 新技术并建立了许多新技术 服务 在过去的五年中 。 ASC将继续支持这些技术和服务，提供 获得关键设备，专业知识和培训，以赋予对啮齿动物模型的专业研究 糖尿病和相关疾病。 ASC由四个实验室组成： 1）大鼠代谢表型实验室：包括评估葡萄糖稳态，全动物 大鼠的代谢评估，身体组成和其他专门代谢评估。 2）光遗传学和行为表型实验室：提供训练和访问光遗传设备 检查对神经电路操纵以及设备的生理和行为反应 衡量相关行为，例如稳态和非整体喂养，活动，奖励和 其他影响和/或受啮齿动物代谢参数调节的行为。 3）连续葡萄糖监测实验室：提供血糖浓度的连续评估 在有意识的，不受限制的啮齿动物中，通过radiotelemetry。这项技术最大程度地减少了处理的压力 啮齿动物并允许对正常喂养方式内以及跨越葡萄糖波动的详细分析 延长的时间框架。 4）胰岛实验室：提供从小鼠和大鼠的胰岛隔离以及胰岛的离体研究（包括枢纽） 和其他内分泌组织。 ASC直接支持MDRC的目标。 ASC提供的服务是唯一的，是 研究糖尿病和相关疾病的啮齿动物模型的重要手段，没有哪个关键方面 与糖尿病有关的研究无法完成。 该核心提供了必要的I NFRATTRUCTURE 对糖尿病和相关疾病的动物模型进行高级，标准化的代谢表型 这是由遗传，药理，饮食或其他扰动引起的。集中设备和服务 以具有成本效益的方式为许多MDRC调查人员加快研究 它们可能没有的代谢技术。