Development of an Automated Diagnostic Platform for SARS-CoV-2 Monitoring in Vulnerable Areas

开发用于脆弱地区 SARS-CoV-2 监测的自动诊断平台

• 批准号: 10321014
• 负责人: Diana Carolina Vanegas-Gamboa
• 金额: $ 42.6万
• 依托单位: CLEMSON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-12-21 至 2023-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10321014
• 关键词: 2019-nCoV; ACE2; Address; Antibodies; Area; Behavior; Biosensing Techniques; Biosensor; COVID-19 detection; COVID-19 monitoring; COVID-19 testing; Coronavirus; Coupled; Detection; Development; Device Designs; Disease Outbreaks; Educational workshop; Electrodes; Elements; Enzymes; Epidemiologic Monitoring; Experimental Designs; Family; Florida; Future; Goals; Human; Human Resources; Immobilization; In Situ; Individual; Infectious Agent; Ionic Strengths; Iowa; Label; Laboratories; Lasers; Lead; Leadership; Liquid substance; Logistics; Microfluidics; Modeling; Molecular Biology; Performance; Printing; Protocols documentation; Public Health; Pump; Readiness; Research; Research Personnel; Resolution; Risk; Role; SARS-CoV-2 spike protein; Saliva; Sampling; Signal Transduction; Stream; Students; System; Temperature; Test Result; Testing; Treatment Protocols; Universities; Validation; Viral; Virulence; Visit; Work; antibody detection; aptamer; base; computer program; computer science; conditioning; cyber physical; design; detection platform; diagnostic platform; emerging pathogen; experimental study; graphene; influenzavirus; meetings; multiplex detection; mutant; prototype; rapid detection; respiratory; saliva sample; seal; symposium; temporal measurement; tool; transmission process

Project Summary The devastation caused by emerging pathogens with fast transmission capacity, such as SARS-CoV-2, has demonstrated the importance of preparedness for future viral outbreaks; this includes the ability for fast deployment of in-situ testing tools and epidemiological surveillance with high temporal and spatial resolution; particularly in places that are most vulnerable to becoming reservoirs of infectious agents. We propose to develop a versatile multiplexing detection platform for SARS-CoV-2 in saliva. The proposed system will integrate signals from different biorecognition elements. Aptamer, antibody, and ACE2 will be immobilized onto laser inscribed graphene electrodes, and detection mechanisms targeting SARS-CoV-2 spike protein will be studied under varying testing conditions (pH, temperature, ionic strength). After determining the operating conditions for enhanced performance of each biosensor, a self-referencing approach will be used between complementary recognition elements (i.e., possible combinations of aptamers, antibodies, and ACE2 enzyme biosensors) to evaluate the effects on test results accuracy (i.e., risk of false-positive and false-negative results). A saliva pre-treatment protocol will be developed to facilitate SARS-CoV-2 testing in human saliva using the multiplex biosensor platform. An open channel microfluidics system will be designed to automatically split and channel a single saliva sample into multiple streams to the biosensors without saturation, biofouling, and pump requirement. The development of a functional and reliable multiplex biosensor system will be useful for addressing longstanding needs in public health as the respiratory Coronavirus family continues its seasonal visits, which may repeat over several decades, much like the occasional visits from the influenza virus, with varying degrees of virulence.

项目摘要 由具有快速传输能力的新兴病原体（例如SARS-COV-2）造成的破坏 证明了准备对未来病毒爆发的重要性；这包括快速的能力 部署原位测试工具和流行病学监视，并具有高时空和空间 解决;特别是在最容易成为传染剂储层的地方。 我们建议为SARIVA中的SARS-COV-2开发一个多功能多路复用检测平台。提议 系统将整合来自不同生物识别元件的信号。适体，抗体和ACE2将是 固定在激光刻有石墨烯电极上，并针对SARS-COV-2的检测机制 将在不同的测试条件（pH，温度，离子强度）下研究尖峰蛋白。后 确定每个生物传感器增强性能的工作条件，这是一种自我参考 互补识别元素之间将使用方法（即可能的组合 适体，抗体和ACE2酶生物传感器）以评估对测试结果准确性的影响（即 假阳性和假阴性结果的风险）。将制定唾液预处理方案 使用多重生物传感器平台在人类唾液中促进SARS-COV-2测试。一个开放频道 微流体系统将设计为自动拆分并将单个唾液样本引导到多个 将无饱和，生物污染和泵要求的生物传感器播放到生物传感器。一个发展 功能和可靠的多重生物传感器系统将有助于满足公众的长期需求 随着呼吸道冠状病毒家族的季节性访问，健康状况可能会重复几次 几十年来，就像偶尔从流感病毒的访问一样，具有不同程度的毒力。

项目成果

An Experimental Framework for Developing Point-of-Need Biosensors: Connecting Bio-Layer Interferometry and Electrochemical Impedance Spectroscopy.

• DOI: 10.3390/bios12110938
• 发表时间: 2022-10-29
• 期刊: Biosensors

Repurposing a SARS-CoV-2 surveillance program for infectious respiratory diseases in a university setting.

• DOI: 10.3389/fpubh.2023.1168551
• 发表时间: 2023
• 期刊: FRONTIERS IN PUBLIC HEALTH
• 影响因子: 5.2
• 作者: King, Kylie L.;Ham, Rachel;Smothers, Austin;Lee, Isaac;Bowie, Tyler;Teetsel, Erika;Peng, Congyue;Dean, Delphine
• 通讯作者: Dean, Delphine

A capacitive laser-induced graphene based aptasensor for SARS-CoV-2 detection in human saliva.

• DOI: 10.1371/journal.pone.0290256
• 发表时间: 2023
• 期刊: PloS one
• 影响因子: 3.7

Ion-selective electrodes based on laser-induced graphene as an alternative method for nitrite monitoring

• DOI: 10.1007/s00604-022-05615-9
• 发表时间: 2023-01
• 期刊: Microchimica Acta
• 影响因子: 5.7
• 作者: Raquel R. A. Soares;Robert G. Hjort;Cicero C. Pola;Dapeng Jing;Victor S. Cecon;J. Claussen;Carmen L. Gomes

Context-Aware Diagnostic Specificity (CADS).

• DOI: 10.3390/bios12020101
• 发表时间: 2022-02-07
• 期刊: Biosensors
• 作者: McLamore ES;Moreira G;Vanegas DC;Datta SPA
• 通讯作者: Datta SPA