STnc520, a virulence-associated regulatory RNA in Salmonella Typhimurium

STnc520，鼠伤寒沙门氏菌毒力相关的调节 RNA

• 批准号: 10307637
• 负责人: Joseph Thomas Wade
• 金额: $ 8.31万
• 依托单位: WADSWORTH CENTER
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-12-01 至 2024-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10307637
• 关键词: Animal Model; Complex; Data; Epithelial Cells; Foundations; Future; Gene Expression; Genes; In Vitro; Infection; Invaded; Maps; Pathogenicity Island; RNA; Role; Salmonella typhimurium; Virulence; Work; base; foodborne illness; gastrointestinal epithelium; in vivo; interest; lambda Spi-1; transcription factor

SUMMARY Salmonella enterica serovar Typhimurium is a major cause of foodborne illness in the USA and worldwide. During the initial stages of infection, S. Typhimurium invades gut epithelial cells. Expression of genes required for invasion is controlled by a group of transcription factors encoded with the SPI-1 pathogenicity island. We have recently mapped the regulatory targets of these transcription factors. The majority of the regulatory interactions we identified have not been previously described. Thus, the regulatory network associated with invasion is considerably more complex than previously appreciated. Moreover, our work strongly suggests an important role for regulatory RNAs during invasion. Of particular interest is the regulatory RNA STnc520, whose expression is coordinated with that of other invasion genes, and which has been suggested to be important for establishing an infection in three animal models, based on data from high-throughput transposon screens. Here, we will determine the requirement for STnc520 for S. Typhimurium to invade epithelial cells, both in vitro and in vivo. We will also identify direct regulatory targets of STnc520. Thus, we will establish the role of STnc520 during invasion, laying the foundation for future studies of this and other regulatory RNAs that promote invasion.

概括 沙门氏菌血清鼠伤寒是在美国和全球造成食源性疾病的主要原因。期间 感染的初始阶段，伤寒链球菌侵入肠道上皮细胞。表达基因所需的基因 入侵受一组用SPI-1致病岛编码的转录因子控制。我们有 最近绘制了这些转录因子的调节目标。大多数监管互动 我们确定没有以前描述过。因此，与入侵相关的监管网络是 比以前所欣赏的要复杂得多。此外，我们的工作强烈暗示了一个重要的角色 用于入侵期间的监管RNA。特别令人感兴趣的是调节RNA STNC520，其表达是 与其他侵袭基因协调，并被认为对于建立一个重要 基于高通量转座子筛网的数据，三个动物模型中的感染。在这里，我们会的 确定STNC520对鼠伤寒沙门氏菌的需求，以侵入体内和体内的上皮细胞。 我们还将确定STNC520的直接监管目标。因此，我们将在期间确定STNC520的作用 入侵，为未来的研究和其他促进入侵的RNA奠定了基础。