Large-scale Optical Recording

大规模光记录

• 批准号: 10302205
• 负责人: William Frost
• 金额: $ 10.55万
• 依托单位: UNIVERSITY OF MASSACHUSETTS AMHERST
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-15 至 2023-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10302205
• 关键词: Anatomy; Anemone; Animal Model; Animals; Aplysia; Area; Back; Behavior; Behavioral; Bite; Brain; Caring; Code; Data Science Core; Diffuse; Dyes; Electrophysiology (science); Environment; Fluorescence; Food; Ganglia; Gills; Goals; Human; Image; Insecta; Investments; Laboratories; Light; Mammals; Methods; Microfluidics; Modality; Modernization; Motor Neurons; Movement; Myenteric Plexus; Nature; Nerve Net; Nervous System Physiology; Nervous system structure; Neurons; Neurosciences; Octopus; Odors; Operating System; Optics; Oral; Peripheral; Play; Positioning Attribute; Preparation; Process; Research; Role; Scheme; Sea Anemones; Sensory; Shapes; Skin; Smell Perception; Stereotyping; Sting Injury; System; Tactile; Tail; Testing; Touch sensation; Transgenic Organisms; Vertebral column; Vision; Work; arm; behavior observation; data access; data exchange; feeding; flexibility; motor control; nudibranch; olfactory bulb glomeruli; operation; optogenetics; relating to nervous system; response; sensory input; somatosensory; tool; two-photon; voltage

PROJECT SUMMARY – Project 3. Large-scale Optical Recording Humans have both centralized and highly distributed nervous systems (e.g., brain and myenteric plexus) that are increasingly appreciated to have important interactions. The nature of those interactions, and especially the degree to which such centralized and distributed nervous systems work together is poorly understood. The proposed work will take advantage of a recently introduced model organism, the marine gastropod Berghia, to begin an exploration of this challenging topic. Berghia has an embodied nervous system organization, consisting of a centralized brain that works with a diffusely distributed peripheral neural plexus. Project 3 will use large scale imaging to explore several features of such embodied processing. A notable feature of this U19 is its investment in generating the first transgenic mollusc for neuroscience. Starting with a backbone of expertise in large scale imaging of neural activity with fast voltage sensitive absorbance dyes, this Project will also employ 1- and 2-photon fluorescence activity imaging, first with dyes and then with GCaMP6f Berghia strains. Additional strains with light-activated channels will enable optogenetics, making possible all-optical electrophysiology in a system employing embodied neural processing. There are four specific aims. Aim 1 will test the hypothesis that Berghia uses two motor control schemes for how it uses its oral tentacles: a traditional one in which the CNS directly drives all aspects of behavior, and an “embodied” control scheme where the CNS cedes responsibility to a highly distributed peripheral neural plexus for certain behaviors. This Aim will further test whether the animal can switch back-and-forth between these two tentacle motor control schemes, depending on the circumstances of the moment. Aims 2-4 are focused on issues related to sensory processing. Aim 2 will explore how somatosensory information is represented in Berghia’s nervous system. Aim 3 will do the same for odor processing, and Aim 4 will assess the degree to which cross-modal sensory interactions and modulation of sensory processing play important roles. Throughout the study, Project 3 will interact extensively with the other 4 Projects of this U19, united by the common goal of revealing general principles and mechanisms of embodied processing as a mode of nervous system function.

项目摘要 - 项目3。大型光学记录 人类具有集中式和高度分布的神经系统（例如大脑和肌丛） 越来越感谢具有重要的相互作用。这些互动的本质，尤其是 这种集中和分布的神经系统共同起作用的程度知之甚少。这 拟议的工作将利用最近引入的模型有机体，海洋局部腹足类Berghia， 开始探索这个挑战主题。 Berghia有一个具体的神经系统组织，包括 与分布分布的周围神经丛一起工作的集中式大脑。项目3将使用大型 缩放成像以探索这种体现处理的几个特征。该U19的一个显着特征是 投资于为神经科学生成第一个转基因软体动物。从专业知识的骨干开始 具有快速电压敏感吸收染料的神经元活性的大型成像，该项目也将采用 1和2光子荧光活性成像，首先是染料，然后是GCAMP6F Berghia菌株。额外的 带有光激活通道的菌株将启用光遗传学，使得在A 采用具体神经加工的系统。有四个具体目标。 AIM 1将检验以下假设 Berghia使用两个电动机控制方案来使用其口服触手：一种传统的CNS 直接推动行为的各个方面，以及“体现”控制方案，中枢神经系统CEDES责任 对某些行为的高度分布的周围神经丛。这个目标将进一步测试动物是否 可以根据情况在这两个触手电动机控制方案之间进行来回切换 这一刻。目标2-4专注于与感官处理有关的问题。 AIM 2将探索如何 体感信息在伯尔吉亚神经系统中表示。 AIM 3对气味也会做同样的事情 处理和AIM 4将评估跨模式感觉相互作用的程度 感官处理扮演着重要角色。在整个研究中，项目3将与其他项目广泛互动 U19的4个项目，由揭示体现的一般原则和机制的共同目标联合 作为神经系统功能的一种处理。