Laser Induced NanoCarbon Multielectrode Arrays for Neurotransmitter Sensing

用于神经递质传感的激光诱导纳米碳多电极阵列

基本信息

批准号：
10288138
负责人：
Mostafa Bedewy
金额：
$ 42.33万
依托单位：
UNIVERSITY OF PITTSBURGH AT PITTSBURGH
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-08-01 至 2024-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10288138
关键词：
3-Dimensional 5-Hydroxytryptophan Acids Acute Adenosine Affect Area Behavior Brain Brain region Calibration Carbidopa Carbon Carbon Nanotubes Chemicals Chemistry Chronic Communities Controlled Environment Corpus striatum structure Coupled Deposition Detection Devices Diagnosis Dopamine Dorsal Drug abuse Electrodes Electron Transport Environment Fast Electron Film Future Growth High temperature of physical object Hippocampus (Brain)Implant In Situ In Vitro Inflammatory Response Injury Kinetics Lasers Learning Light Manuals Measurement Measures Mechanics Mediator of activation protein Memory Mental Depression Methods Microelectrodes Microfabrication Modality Modulus Morphology Motivation Movement Nanostructures Nervous System Physiology Neurotransmitters Newspapers Parkinson Disease Pathologic Pattern Performance Periodicity Pharmacology Phase Polymers Porosity Positioning Attribute Printing Process Production Property Rattus Reproducibility Scanning Serotonin Site Source Structure Surface Techniques Technology Thick Time Tissues Validation Work Writing base biomaterial compatibility carbon fiber chemical property cost density detection sensitivity effectiveness evaluation effectiveness validation electric impedance flexibility graphene improved in vivo internal control invention irradiation multi-electrode arrays nanoscale nervous system disorder neurochemistry neuropsychiatric disorder neurotransmission optogenetics scale up sensor temporal measurement

项目摘要

Project Summary The real-time measurement of neurotransmitters in vivo in living brain is of utmost importance for understanding brain functions in normal and pathological conditions and to improve diagnosis and treatments of neurological and neuropsychiatric diseases. High surface area carbon (HSAC), or nanocarbon, has been considered the ideal material for electrochemical detection of neurotransmitters, due to its outstanding electrochemical properties and chemical inertness. However, HSAC microelectrode arrays (MEAs) are difficult to fabricate, and the extreme environments needed for the nanocarbon synthesis limit the choice of substrate to rigid materials that can withstand high temperatures. Moreover, chemical doping to improve electrochemical sensing also requires high-temperature post-synthesis processing. Thus, there is an unmet need for fabricating implantable HSAC MEAs on flexible substrates with tunability of morphology and chemistry, for multisite measurements of neurotransmitters at different temporal resolutions (ms to min), within and across brain regions (µm to mm). To fill this gap, this project introduces a new laser-induced nanocarbon (LINC) fabrication technique, capable of patterning customizable types of HSAC on-demand directly on flexible polymers. LINC is a new direct-write process with the unprecedented ability for bottom-up growth of nanocarbons on polymers that act as the carbon source upon laser irradiation. Our inventive approach enables for the first time, a fast, low-cost, batch-fabrication of HSAC MEAs in a highly reproducible way, without the need of high-temperature carbon synthesis, or multistep microfabrication processes. Importantly, LINC allows in situ precise control of the nanocarbon atomic structure, nanoscale morphology, and surface chemistry. Thus, our HSAC MEAs will be tailored for high-sensitivity electrochemical detection of different neurotransmitters using two different electrochemical technique: fast scan cyclic voltammetry (FSCV), for capturing of fast phasic dynamics, and square wave voltammetry (SVW) for detecting tonic levels. Following a meticulous in vitro optimization, we will determine the effectiveness of the proposed HSAC MEA in performing electrochemical sensing of electroactive neurotransmitters for acute in vivo detection of 1) tonic (via SWV) and 2) electrically evoked (via FSCV) dopamine and serotonin release in the rat dorsal striatum and in the hippocampus (CA2 region) of rat brain, respectively or simultaneously. The successful completion of this project will provide 1) a cutting-edge technology with the potential to revolutionize the state- of-the-art of nanocarbon-based MEA fabrication for neurochemical applications, and 2) will provide the scientific community with a platform for unprecedented studies of neurotransmitters and their interactions in normal and pathological brain conditions.

项目摘要生物大脑体内神经递质的实时测量至关重要了解正常和病理状况下的大脑功能，并改善神经和神经精神疾病。高表面积碳（HSAC）或纳米碳被认为是电化学的理想材料由于其出色的电化学特性和化学惰性，神经递质的检测。但是，HSAC微电极阵列（MEA）很难制造，并且需要的极端环境对于纳米碳合成，将底物的选择限制为可以承受高温的刚性材料。此外，化学掺杂以改善电化学感官也需要高温后合成加工。那就是在柔性基板上制造可植入的HSAC测量的需求未满足形态和化学的可调性，用于不同临时性神经递质的多站点测量在大脑区域内和跨大脑区域（µm至mm）内部和跨度。为了填补这一空白，该项目引入了一种新的激光诱导的纳米碳（LINC）制造技术直接在柔性聚合物上对可定制类型的HSAC按需进行构图。 LINC是新的直接作品具有前所未有的能力的纳米碳自下而上生长的能力，可作为碳的聚合物激光照射后的来源。我们的发明方法首次实现了快速，低成本的批量制作以高度可重现的方式进行HSAC测量，而无需高温碳合成或多步微加工过程。重要的是，LINC允许原位精确控制纳米碳原子结构，纳米级形态和表面化学。那就是我们的HSAC尺寸将针对高敏性量身定制使用两种不同的电化学技术对不同神经递质的电化学检测：快速扫描循环伏安法（FSCV），用于捕获快速阶段动力学和方波伏安法（SVW）检测补品水平。经过细致的体外优化，我们将确定拟议的HSAC MEA在进行急性体内的电活性神经递质的电化学感知中检测1）补品（通过SWV）和2）在大鼠中以电子诱发（通过FSCV）多巴胺和5-羟色胺释放背侧纹状体和大鼠脑的海马（Ca2区域）中或简单。成功该项目的完成将提供1）尖端技术，有可能彻底改变国家 - 用于神经化学应用的纳米碳基MEA制造的艺术，2）将提供科学社区具有空前研究神经递质及其在正常和正常和相互作用的平台病理大脑条件。

项目成果

期刊论文数量（3）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Effects of central nervous system electrical stimulation on non-neuronal cells.

DOI：
10.3389/fnins.2022.967491
发表时间：
2022
期刊：
FRONTIERS IN NEUROSCIENCE
影响因子：
4.3
作者：
Williams, Nathaniel P.;Kushwah, Neetu;Dhawan, Vaishnavi;Zheng, Xin Sally;Cui, Xinyan Tracy
通讯作者：
Cui, Xinyan Tracy

Stable in-vivo electrochemical sensing of tonic serotonin levels using PEDOT/CNT-coated glassy carbon flexible microelectrode arrays.