Bioelectrochemistry at the Nanoscale: Fundamentals and Applications

纳米级生物电化学：基础知识和应用

• 批准号: 10276094
• 负责人: Lior Sepunaru
• 金额: $ 34.7万
• 依托单位: UNIVERSITY OF CALIFORNIA SANTA BARBARA
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-23 至 2026-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10276094
• 关键词: Antibiotics; Area; Biochemical; Biological; Biosensor; Catalysis; Charge; Chemicals; Complex; Detection; Development; Devices; Electrochemistry; Enzymes; Future; Generations; Goals; Individual; Industrialization; Kinetics; Measures; Methods; Molecular; Oxidation-Reduction; Pathogen detection; Process; Reaction; Research; Side; System; Techniques; Thermodynamics; Time; Vision; communicable disease diagnosis; design; individual response; innovation; nanoscale; operation; physical property; point of care; programs; rapid diagnosis; sensor; tool

Project Summary Key questions of the 21st century in medicinal and biochemical areas are focused on the intimate operation and functionality of biomolecule at the molecular level. By understanding the physical properties of biomolecules at the nanoscale we can envision how to control them and use them for various applications. The proposal below is aimed to promote these key questions via the use of nanoscale electrochemistry. I am very excited to share with you some of our preliminary results and future vision dealing with both the applied and fundamental sides of Bioelectrochemistry. The first part of the proposal is focused on merges the lab expertise in nanoelectrochemistry and charge transport phenomena and focuses on an innovative method to measure the electronic response of individual enzymes during catalysis. By that, we hope to unravel by electronic means the way that enzymes operate and fluctuate with respect to their catalytic activity at the molecular level. Gained information can guide us towards the control and design of enzymes both from fundamental aspects and industrial applications. The second part of the research program offers a template for a paradigm shift in the way we think about electrochemical biosensors. We propose to construct a chemically amplified electrochemical system that enables the detection and analytical sizing of insulating materials, one at a time. We plan to use this experimental approach for designing a sensor for rapid pathogen detection. The main goal here is to innovate a sensor that can be used in a personalized fashion, without the need of complex device operation. Realization of such sensor can potentially mitigate the number of unnecessary antibiotics given at the point of care.

项目摘要 21世纪的药用和生化区域的关键问题集中在亲密操作上 生物分子在分子水平上的功能。通过了解的物理特性 纳米级的生物分子我们可以设想如何控制它们并将其用于各种应用。 下面的提案旨在通过使用纳米级电化学来促进这些关键问题。我 很高兴与您分享我们的一些初步结果和未来的愿景 生物电化学的应用和基本方面。提案的第一部分集中于合并 实验室专业知识在纳米电子化学和收费运输现象方面，专注于创新性 测量催化过程中各个酶的电子响应的方法。这样，我们希望 电子拆卸是指酶相对于其催化的方式和波动的方式 分子水平的活性。获得的信息可以指导我们控制和设计酶 来自基本方面和工业应用。 研究计划的第二部分为我们考虑的方式提供了模板的模板 电化学生物传感器。我们建议构建一个化学扩增的电化学系统 一次性材料的检测和分析大小一次。我们计划使用这个 设计用于快速病原体检测的传感器的实验方法。这里的主要目标是 创新可以以个性化方式使用的传感器，而无需复杂的设备操作。 实现此类传感器可以潜在地减轻当时给出的不必要抗生素的数量 照顾。