Bioelectrochemistry at the Nanoscale: Fundamentals and Applications

纳米级生物电化学：基础知识和应用

• 批准号: 10276094
• 负责人: Lior Sepunaru
• 金额: $ 34.7万
• 依托单位: UNIVERSITY OF CALIFORNIA SANTA BARBARA
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-23 至 2026-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10276094
• 关键词: Antibiotics; Area; Biochemical; Biological; Biosensor; Catalysis; Charge; Chemicals; Complex; Detection; Development; Devices; Electrochemistry; Enzymes; Future; Generations; Goals; Individual; Industrialization; Kinetics; Measures; Methods; Molecular; Oxidation-Reduction; Pathogen detection; Process; Reaction; Research; Side; System; Techniques; Thermodynamics; Time; Vision; communicable disease diagnosis; design; individual response; innovation; nanoscale; operation; physical property; point of care; programs; rapid diagnosis; sensor; tool

Project Summary Key questions of the 21st century in medicinal and biochemical areas are focused on the intimate operation and functionality of biomolecule at the molecular level. By understanding the physical properties of biomolecules at the nanoscale we can envision how to control them and use them for various applications. The proposal below is aimed to promote these key questions via the use of nanoscale electrochemistry. I am very excited to share with you some of our preliminary results and future vision dealing with both the applied and fundamental sides of Bioelectrochemistry. The first part of the proposal is focused on merges the lab expertise in nanoelectrochemistry and charge transport phenomena and focuses on an innovative method to measure the electronic response of individual enzymes during catalysis. By that, we hope to unravel by electronic means the way that enzymes operate and fluctuate with respect to their catalytic activity at the molecular level. Gained information can guide us towards the control and design of enzymes both from fundamental aspects and industrial applications. The second part of the research program offers a template for a paradigm shift in the way we think about electrochemical biosensors. We propose to construct a chemically amplified electrochemical system that enables the detection and analytical sizing of insulating materials, one at a time. We plan to use this experimental approach for designing a sensor for rapid pathogen detection. The main goal here is to innovate a sensor that can be used in a personalized fashion, without the need of complex device operation. Realization of such sensor can potentially mitigate the number of unnecessary antibiotics given at the point of care.

项目概要 21世纪医药生化领域的关键问题集中在亲密操作上 以及分子水平上生物分子的功能。通过了解物理特性 对于纳米级的生物分子，我们可以设想如何控制它们并将它们用于各种应用。 以下提案旨在通过使用纳米级电化学来促进这些关键问题的解决。我 我非常高兴与您分享我们在这两个领域的一些初步成果和未来愿景 生物电化学的应用和基础方面。提案的第一部分重点关注合并 实验室在纳米电化学和电荷传输现象方面的专业知识，并专注于创新 测量催化过程中单个酶的电子响应的方法。借此，我们希望 通过电子手段揭示酶的运作方式及其催化波动的方式 分子水平的活性。获得的信息可以指导我们控制和设计酶 无论是从基础方面还是工业应用方面。 研究计划的第二部分为我们思考方式的范式转变提供了一个模板 电化学生物传感器。我们建议构建一个化学放大电化学系统 能够一次检测和分析绝缘材料的尺寸。我们计划使用这个 设计用于快速病原体检测的传感器的实验方法。这里的主要目标是 创新一种可以个性化方式使用的传感器，无需复杂的设备操作。 这种传感器的实现可能会减少此时施用不必要的抗生素的数量 的照顾。