DCEG- Molecular Assays for epidemiology studies

DCEG-流行病学研究的分子测定

基本信息

批准号：
10273216
负责人：
LEONARD FREEDMAN
金额：
$ 302.89万
依托单位：
LEIDOS BIOMEDICAL RESEARCH, INC.
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-08-31 至 2021-08-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10273216
关键词：
Biological Biological Assay Biological Process Blood specimen CLIA certified Cell Line Cells Cervical Clinical Research Contracts Cryopreservation Cryopreserved Cell Cytology DNA Detection Diagnostics Research Division of Cancer Epidemiology and Genetics Epidemiology Erythrocytes Feces Fibroblasts Flow Cytometry Fluorescence-Activated Cell Sorting Freeze Drying Freezing Hormones Human Human Herpesvirus 4 Immunologic Monitoring Immunologic Tests Immunologics In Vitro Infrastructure Laboratories Liquid substance Malignant neoplasm of cervix uteri Mass Spectrum Analysis Measurement Molecular Molecular Epidemiology Mutation Detection Nitrogen Nucleic Acids Paraffin Embedding Pathogen detection Pathogenesis Peripheral Blood Mononuclear Cell Phenotype Plasma Polymorphism Analysis Preparation Prevention Process Proteins Quality Control RNA Reporting Research Personnel Scientist Serum Services Single Nucleotide Polymorphism Specimen Specimen Handling Spectrophotometry Structure Techniques Technology Tissue Embedding Tissues Urine Virus Whole Blood anticancer research biobank bioprocess blood fractionation cancer epidemiology cell immortalization cold temperature epidemiology study established cell line instrumentation molecular diagnostics programs quality assurance repository small molecule tumor

项目摘要

Activities performed within this project provide DNA extraction, alliquoting, bioprocessing of human epidemiology specimens for cancer research. Several facilities are utilized to process human specimens and to provide the necessary infrastructure to support human specimen processing and storage in support of epidemiology cancer research. The Mass Spectrometry Center is equipped with the latest in mass spectrometry (MS) instrumentation for analyzing both small and large protein biomolecules, including endogenous hormones. The Center offers corresponding expertise in detection, quantitation, structure confirmation, and mass measurements. The Center also educates scientists on the use, interpretation, and applications of MS technologies. The CLIA-certified Molecular Diagnostics Laboratory provides state-of-the-art clinical and research diagnostics, including mutation detection, single nucleotide polymorphism analysis, and pathogen detection. The Human Immunological Monitoring Laboratory supports cervical cancer research by examining human host immunological parameters potentially important in the pathogenesis or prevention of virus-related tumors. The laboratory's capabilities include: receipt and processing of human specimens, including whole blood and cervical specimens; cryopreservation, DNA extraction and Epstein-Barr virus transformation of peripheral blood mononuclear cells (PBMC); immunological testing, including a variety of in vitro PBMC functional assays; HLA and PBMC phenotyping by flow cytometry (fluorescence activated cell sorting – FACS); temporary low temperature storage of biological specimens and transfer to the DCEG biorepository; electronic tracking of biospecimens and reporting of assay results to DCEG investigators; maintain quality control/quality assurance programs; apply state-of-the-art techniques, as they become available. The Bioprocessing Laboratory processes biological specimens prior to their storage in the DCEG repository or dispersal to collaborators. The processing laboratory provides: processing and freezing of biological specimens, including whole blood, serum, plasma, urine, and feces; lyophilization of specimens; flash-freezing of tissue in liquid nitrogen; separation and viable cryopreservation of cell lines, including the routine assessment of viability; cryopreservation of red blood cells to maintain enzymatic activity; establishment of cell lines; large scale propagation of cell lines; nucleic acid extraction; distribution of specimens. The DNA Extraction Laboratory, a part of the CGR, extracts DNA from whole blood specimens, blood fractions, transformed lymphoblastic lines, cultured fibroblasts, tumor specimens, buccal cells, paraffin-embedded tissue, Guthrie cards, cytology preparations, or other biological specimens. Activities performed within this project provide DNA extraction, alliquoting, bioprocessing of human epidemiology specimens for cancer research.

该项目中执行的活动提供了DNA提取，分析，对癌症研究的人类流行病学标本的生物处理。几种设施用于处理人类标本，并提供必要的基础设施，以支持人类标本的加工和存储以支持流行病学癌症研究。质谱中心与最新的质谱法（MS）仪器相等，用于分析包括内源性恐怖在内的小蛋白质生物分子。该中心提供相应的检测，定量，结构确认和质量测量的专业知识。该中心还对科学家进行了MS技术的使用，解释和应用。经CLIA认证的分子诊断实验室提供了最先进的临床和研究诊断，包括突变检测，单核苷酸多态性分析和病原体检测。人类免疫学监测实验室通过研究人类宿主免疫学参数可能在病毒相关肿瘤的发病机理或预防中可能重要，从而支持宫颈癌研究。实验室的功能包括：人类标本的收据和加工，包括全血和宫颈标本；周围血液单核细胞的冷冻保存，DNA提取和爱泼斯坦 - 巴尔病毒转化（PBMC）；免疫学测试，包括各种体外PBMC功能测定；通过流式细胞仪（荧光激活的细胞分选 - FACS）进行HLA和PBMC表型；生物标本的暂时低温存储并转移到DCEG生物座位上；对生物测量的电子跟踪和对DCEG研究者的测定结果报告；维护质量控制/质量保证计划；应用最新技术，因为它们可用。生物处理实验室工艺在存储DCEG存储库之前或分散到合作者之前将其生物标本。加工实验室提供：生物标本的加工和冻结，包括全血，血清，血浆，尿液和粪便；标本的冻干；液氮液体中组织的闪光冻结；细胞系的分离和生存的冷冻保存，包括常规的生存能力评估；红细胞的冷冻保存以维持酶活性；建立细胞系；细胞系的大规模传播；核酸提取；标本的分布。 DNA提取实验室是CGR的一部分，从全血标本，血液馏分，转化的淋巴细胞，培养的成纤维细胞，肿瘤标本，颊细胞，石蜡包含的组织，Guthrie卡，Guthrie卡，细胞学准备工作，或其他生物学标本中提取DNA。该项目中执行的活动提供了DNA提取，分析，对癌症研究的人类流行病学标本的生物处理。