Histology, Microscopy and Image Analysis Core

组织学、显微镜和图像分析核心

基本信息

批准号：
10219293
负责人：
JAMES J TOMASEK
金额：
$ 14.8万
依托单位：
UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
依托单位国家：
美国
项目类别：
财政年份：
2017
资助国家：
美国
起止时间：
2017-07-01 至 2023-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10219293
关键词：
Animal Model Animals Archives Biomedical Research Budgets Businesses Cancer Center Cell physiology Cells Centers of Research Excellence Complement Computer software Confocal Microscopy Control Animal Core Facility Cryopreserved Tissue Development Diabetes Mellitus Doctor of Philosophy Ensure Equipment Floor Freezing Funding Goals Grant Growth Health Sciences Histologic Histology Image Image Analysis In Vitro Laboratories Lasers Letters Mentors Methodology Methods Microscope Microscopic Microscopy Nomarski Interference Contrast Microscopy Oklahoma Organ Paraffin Paraffin Embedding Pathologic Phase Phase-Contrast Microscopy Pilot Projects Postdoctoral Fellow Preparation Publications Research Research Personnel Scanning Science Scientist Services Slide Stains Technology Tissue Banks Tissues Training Universities Wages animal tissue cost effective design diabetic graduate student human tissue instrumentation laboratory equipment laser capture microdissection live cell imaging microscopic imaging multi-photon novel quantitative imaging response sample fixation tissue preparation tissue processing tool translational cancer research

项目摘要

PROJECT SUMMARY The Histology, Microscopy and Image Analysis Core is designed to meet the needs of Promising Junior Investigators, Pilot Project Researchers, and Funded Diabetes Investigators by assisting them in studying diabetic human and animal tissues utilizing state-of-the-art histologic and microscopic technologies, as well as assisting them with and providing them with a variety of types of microscopy required for in vitro cellular microscopic analysis. Tissue preparation requires a specialized laboratory and equipment, while state-of-the-art microscopes are outside the budget of most laboratories. The objective of the HMIA Core is twofold. One is to centralize histological preparation of tissues and provide access to specialized equipment for preparing and cutting tissues. The second is to maintain and provide training on and access to a variety of different types of state-of-the-art microscopes and to quantitative image analysis software. The core will provide tissue processing, embedding, sectioning, and histochemical staining of mounted slides, for both paraffin embedded and cryo-preserved tissue preparations. In addition, the core will provide quality state-of-the-art instrumentation and expertise for investigators to obtain microscopic images of publication quality using bright field, phase contrast, and Nomarski DIC microscopy, epifluorescence microscopy, confocal and multi-photon microscopy, laser capture microdissection microscopy, and live-cell imaging microscopy. To complement the microscopy component of this Core, assistance and training with a variety of quantitative image analysis tools is provided. The signup, billing, and tracking of Core use is all done using iLabs software. The Core comprises 500 sq ft on the 5th floor of the Biomedical Sciences Building for the histology functions and 150 sq ft on the 2nd floor of the Biomedical Research Building for imaging functions. A business plan is in place so that by the end of the COBRE Phase III the core will be financially stable and ready to integrate into the OUHSC Research Core Facilities network.

项目摘要组织学，显微镜和图像分析核心旨在满足有前途的初级研究人员，试点项目研究人员和资助的糖尿病研究人员通过协助他们研究糖尿病性人类和动物组织利用最先进的组织组织学和微观技术，并协助它们并为其提供体外细胞显微镜分析需要多种类型的显微镜。组织准备工作需要专门的实验室和设备，而最先进的显微镜超出了大多数实验室的预算。 HMIA核心的目的是双重的。一个是集中组织的组织学准备，并为专用设备提供访问准备和切割组织。第二个是维护和提供有关和访问的培训多种不同类型的最先进的显微镜和定量图像分析软件。核心将提供组织加工，嵌入，切片和组织化学用于嵌入的石蜡和冷冻保存的组织制剂的安装载玻片的染色。此外，核心将为最先进的工具和专业知识提供研究人员使用明亮的场，阶段获得出版质量的显微镜图像对比度和Nomarski DIC显微镜，表落荧光显微镜，共聚焦和多光子显微镜，激光捕获显微镜显微镜和活细胞成像显微镜。到补充该核心的显微镜成分，辅助和训练提供定量图像分析工具。核心使用的注册，计费和跟踪都是使用ILABS软件完成了。核心在生物医学的5楼上包括500平方英尺生物医学2楼的组织学功能的科学构建和150平方英尺成像功能的研究大楼。制定了商业计划，以便在索贝尔第三阶段核心将在财务上保持稳定，并准备融入OUHSC 研究核心设施网络。