Core B: Shared Resource Core For Characterizing Antibody Responses To SARS-CoV-2 And Other Pathogenic Human Coronaviruses

核心 B：用于表征对 SARS-CoV-2 和其他致病性人类冠状病毒的抗体反应的共享资源核心

• 批准号: 10222242
• 负责人: Aravinda M. DeSilva
• 金额: $ 52.88万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-30 至 2022-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10222242
• 关键词: 2019-nCoV; Affinity; Antibodies; Antibody Response; Antigen Receptors; Antigens; B-Lymphocytes; Biological Assay; Containment; Coronavirus; Coronavirus spike protein; Detection; Enzyme-Linked Immunosorbent Assay; Exposure to; Fc Receptor; Goals; Gold; Health care facility; Immune; Immune Sera; Immunoglobulin Genes; Individual; Infection; Label; Length; Mammalian Cell; Measures; Methods; Middle East Respiratory Syndrome; N-terminal; North Carolina; Nucleocapsid; Paralysed; Pathogenicity; Pattern; Production; Property; Proteins; Recombinants; Research; Research Project Grants; Resource Sharing; SARS coronavirus; Science; Serologic tests; Serological; Serum; Severe Acute Respiratory Syndrome; Site; Sorting - Cell Movement; Specificity; Structure; System; Testing; Vaccines; Viral; Viral Pathogenesis; Virus; base; human coronavirus; milligram; pandemic disease; receptor binding

Abstract High-quality recombinant antigens and reliable and accurate SARS-CoV-2 serological assays are urgently needed for surveillance and for defining the immune mechanisms of viral pathogenesis, viral clearance, and protection against reinfection. Core B of the UNC SARS-CoV-2 Serological Sciences Center of Excellence will apply state-of-the-art strategies to establish methods for the production of milligram quantities of homogeneous recombinant spike protein CoV antigens with versatile tags and site-specific labels and use these antigens to develop sensitive and reliable singleplex and multiplex Ab detection assays. Core B will interact and support all the projects driven by the center and collaborators' by providing recombinant antigens and serological assays to characterize the serum antibody response, elucidate Fc effector functional profiles, and isolate and sequence antigen-specific immunoglobulin gene repertoire.

抽象的 高质量的重组抗原以及可靠，准确的SARS-COV-2血清学测定 监测和定义病毒发病机理，病毒清除和 防止再感染。 UNC SARS-COV-2血清学科学卓越中心的核心B将 采用最先进的策略来建立生产毫克同质量的方法 具有多功能标签和特定地点标签的重组尖峰蛋白COV抗原，并将这些抗原用于 开发敏感且可靠的单重包和多重AB检测测定法。核心B将互动和支持 由中心和合作者驱动的项目通过提供重组抗原和血清学测定 表征血清抗体反应，阐明FC效应子功能曲线以及分离和序列 抗原特异性免疫球蛋白基因库。