Biocompatible Graphene Quantum Dots for Noninvasive Near-infrared Bioimaging

用于无创近红外生物成像的生物相容性石墨烯量子点

基本信息

批准号：
10203022
负责人：
Anton Naumov
金额：
$ 41.01万
依托单位：
TEXAS CHRISTIAN UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-08-01 至 2024-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10203022
关键词：
Address Adverse effects Animal Experimentation Animal Model Animal Testing Animals Area Biological Biological Assay Biosensing Techniques Biotechnology Cell Survival Cells Chemicals Clinical Complex Contrast Media Defect Development Diagnosis Diagnostic Drug Delivery Systems Drug Transport Exhibits Fluorescence Fluorescence Microscopy Future Glucosamine Health Human Image Image-Guided Surgery In Situ In Vitro Light Magnetic Resonance Imaging Medical Students Microscopic Minor Mus Natural graphite Near-infrared optical imaging Operative Surgical Procedures Ophthalmology Optics Organ Oxides Oxygen Patients Penetration Pharmaceutical Preparations Process Property Quantum Dots Rare Earth Metals Research Science Site Slice Structure System Techniques Testing Therapeutic Time Tissue imaging Tissues Toxic effect UV Radiation Exposure Visible Radiation Visualization Work animal imaging base bioimaging biomaterial compatibility career drug testing feasibility testing fluorescence imaging fluorophore functional group graphene image guided image-guided drug delivery imaging agent imaging platform in vivo in vivo imaging in vivo imaging system infrared microscopy luminescence microwave electromagnetic radiation mouse model nanomaterials near infrared dye novel oxidation ozone exposure physical property quantum real-time images success targeted treatment therapeutic evaluation tool tumor undergraduate student uptake water solubility

项目摘要

Project Summary/Abstract Near-infrared (NIR) imaging is one of the most rapidly developing perspective techniques in biomedical diagnostics enabling 10 - 100 times deeper tissue imaging than regular visible fluorescence techniques. There is a significant clinical need for such imaging: high-depth CT and MRI imaging strategies are complex and less suitable for low-depth biosensing and intraoperative applications. Furthermore, in animal studies NIR imaging can enable in situ fluorescence tracking of tested therapeutics or tumor visualization inside a living small animal. However, as NIR imaging field is still developing, there is a lack of biocompatible and multifunctional platforms suitable for imaging, sensing and even drug transport highly desired in animal studies. In this project we will develop and test the feasibility of a novel NIR imaging platform based on graphene quantum dots (GQDs) addressing the critical needs of NIR bioimaging: fluorescence in the NIR (above 950 nm) with NIR excitation, high biocompatibility, photostability, and capabilities for drug tracing and in vivo NIR imaging in live animal models. This will be implemented in 3 steps: (1) synthesizing NIR-emissive GQDs via bottom-up and top-down approaches and characterizing their optical and physical properties, (2) testing GQDs for NIR in vitro imaging and assessing their biocompatibility and (3) performing in vivo imaging of GQD NIR fluorescence in live animals as well as ex vivo organ tissue imaging quantifying GQD content in excised organs. We will concomitantly assess potential adverse effects to select non-toxic GQD candidates. Throughout this process a variety of synthesized GQD structures will undergo rigorous 3-step selection for the capabilities of (1) high yield NIR fluorescence, (2) high biocompatibility and NIR imaging in cells, (3) NIR imaging inside a live sedated animal with minimal/no toxicity. The selected GQD candidates will enable in vivo animal testing of therapeutic delivery with noninvasive real-time image tracking reducing the number of animals sacrificed. In a longer term, we expect these GQD platforms to become a basis of the noninvasive NIR in vivo sensing in patients. As a result, the proposed initiative will address several significant areas of biotechnology and its development will ultimately contribute to the improvement of human health.

项目摘要/摘要近红外（NIR）成像是最快发展的透视技术之一生物医学诊断可实现10至100倍的组织成像，比常规可见荧光技术。对这种成像的临床需求很大：高深度CT和 MRI成像策略很复杂，不适合低深度生物传感和术中。申请。此外，在动物研究中，NIR成像可以实现原位荧光跟踪活的小动物中测试的治疗剂或肿瘤可视化。但是，作为NIR成像田野仍在发展，缺乏适合生物相容性和多功能平台在动物研究中高度期望的成像，传感甚至药物转运。在这个项目中，我们将根据基于新颖的NIR成像平台的可行性开发和测试石墨烯量子点（GQD）满足了NIR生物成像的关键需求：荧光中的荧光具有NIR激发，高生物相容性，光稳定性和能力的NIR（950 nm以上）用于现场动物模型中的药物追踪和体内NIR成像。这将通过3个步骤实施：（1）通过自下而上和自上而下的方法合成NIR隔离的GQD并表征它们的光学和物理特性，（2）测试用于NIR体外成像的GQD并评估其生物相容性和（3）在活动物中对GQD NIR荧光进行体内成像作为离体器官组织成像，量化了切除器官中的GQD含量。我们会同时进行评估对选择无毒GQD候选者的潜在不利影响。在整个过程中合成的GQD结构将对（1）高的功能进行严格的3步选择产生NIR荧光，（2）细胞中的高生物相容性和NIR成像，（3）活体内的NIR成像具有最小/无毒性的镇静动物。选定的GQD候选者将启用体内动物通过非侵入性实时图像跟踪的治疗交付测试，以减少数量动物牺牲了。从长远来看，我们希望这些GQD平台将成为患者的非侵入性NIR体内感测。结果，拟议的倡议将解决一些生物技术及其发展的重要领域最终将有助于改善人类健康。

项目成果

期刊论文数量（4）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Detection of Pancreatic Cancer miRNA with Biocompatible Nitrogen-Doped Graphene Quantum Dots.

DOI：
10.3390/ma15165760
发表时间：
2022-08-20
期刊：
Materials (Basel, Switzerland)
影响因子：
0
作者：
通讯作者：

Automated Approach to In Vitro Image-Guided Photothermal Therapy with Top-Down and Bottom-Up-Synthesized Graphene Quantum Dots.

DOI：
10.3390/nano13050805
发表时间：
2023-02-22
期刊：
Nanomaterials (Basel, Switzerland)
影响因子：
0
作者：
Lee B;Stokes GA;Valimukhametova A;Nguyen S;Gonzalez-Rodriguez R;Bhaloo A;Coffer J;Naumov AV
通讯作者：
Naumov AV

Cancer Therapeutic siRNA Delivery and Imaging by Nitrogen- and Neodymium-Doped Graphene Quantum Dots.

DOI：
10.1021/acsbiomaterials.3c00369
发表时间：
2023-05
期刊：
ACS biomaterials science & engineering
影响因子：
5.8
作者：
Alina Valimukhametova;B. Lee;U. C. Topkiran;Klara Gries;R. Gonzalez-Rodriguez;J. Coffer;G. Akkaraju;A. Naumov
通讯作者：
Alina Valimukhametova;B. Lee;U. C. Topkiran;Klara Gries;R. Gonzalez-Rodriguez;J. Coffer;G. Akkaraju;A. Naumov

Doped Graphene Quantum Dots as Biocompatible Radical Scavenging Agents.