Center on Macromolecular Dynamics by NMR Spectroscopy

核磁共振波谱大分子动力学中心

• 批准号: 10194529
• 负责人: ARTHUR G PALMER
• 金额: $ 124.78万
• 依托单位: NEW YORK STRUCTURAL BIOLOGY CENTER
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-06-01 至 2022-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10194529
• 关键词: 2,4-Dinitrophenol; Address; Amides; Automobile Driving; Basic Science; Biological; Biological Process; Biology; Biomedical Research; Biomedical Technology; Catalysis; Chemical Dynamics; Chemicals; Collaborations; Communities; Computing Methodologies; Coupling; Data; Data Analyses; Databases; Degenerative Disorder; Deposition; Detection; Development; Educational workshop; Emerging Technologies; Ensure; Enzymes; Equipment; Experimental Designs; Freezing; Future; Geography; Health; Human; Individual; Infrastructure; Investigation; Kinetics; Laboratories; Ligands; Magic; Malignant Neoplasms; Measurement; Medical; Medicine; Metabolic Diseases; Methods; Modernization; Molecular Conformation; Motion; NMR Spectroscopy; Nature; New York; Nuclear; Nucleic Acid Conformation; Office of Administrative Management; Operating System; Phase; Physiologic pulse; Pilot Projects; Preparation; Procedures; Process; Proteins; Protocols documentation; Protons; Publications; Relaxation; Research; Research Personnel; Residual state; Resources; Rotation; Sampling; Services; Site; Specialist; System; Techniques; Technology; Technology Transfer; Testing; Time; Training; Training Activity; Training Programs; Translating; Work; biological research; biomacromolecule; data acquisition; data analysis pipeline; density; design; high risk; improved; interest; macromolecule; magnetic field; medical specialties; meetings; method development; molecular recognition; program dissemination; programs; protein folding; research and development; solid state nuclear magnetic resonance; structural biology; symposium; technology development; technology research and development; web site

This proposal describes the need for and outline of a Biomedical Technology Research Resource entitled Center on Macromolecular Dynamics by NMR Spectroscopy (CoMD/NMR) and situated at the New York Structural Biology Center. The focus of CoMD/NMR is technical development and application of NMR spin relaxation and associated methods for characterizing protein and nucleic acid conformational dynamics in biological processes including ligand recognition, allosterism, catalysis, and folding. The central challenge addressed by CoMD/NMR is to break down the high activation barrier to using advanced NMR spectroscopic and computational methods and thus to make available the benefits of these sophisticated approaches to the wider biological research community. CoMD/NMR is the platform for meeting this challenge through Technological Research and Development (TR&D) Projects, Driving Biomedical Projects (DBPs), Collaboration and Service, Training, and Dissemination components of the Resource. The TR&D component of CoMD/NMR will develop new and extended methods for characterizing and interpreting macromolecular dynamics, with a strong focus on expanding the general ranges of time scales and masses of targets accessible for study. The dual needs for integrating experimental, computational, and theoretical methods (within the TR&D component) and for interfacing with key biological researchers (within the DBP and Collaboration components) make CoMD/NMR essential. In particular, CoMD/NMR addresses four primary obstacles: (i) future technology developments requires combined advances in sample preparation, NMR hardware and methods development, and integration with computer methods; (ii) access to NMR spectrometers at multiple static magnetic fields, as well as specialty equipment such as dynamic nuclear polarization and field cycling probes, is key to many emerging methods; (iii) transfer of technology to the general biological community has been hindered by the complexity of the methods, absence of integrated pipelines to facilitate data acquisition and analysis by non- specialists, and lack of infrastructure in individual laboratories to translate these methods for general use; and (iv) biologists with research programs that would benefit from these methods are unaware of this potential and lack expertise to incorporate these approaches into their own research. Technology development proceeds most effectively when driven by challenging applications; accordingly, CoMD/NMR will work closely with outstanding local and national investigators in DBPs designed to motivate and test emerging technology. The resources of CoMD/NMR will be broadly available to the biomedical research community through collaboration and service activities together with expansive training and dissemination programs. The basic research developments of CoMD/NMR will impact a diverse range of biological research with human health relatedness, including degenerative diseases, metabolic disorders, and cancer.

该建议描述了生物医学技术研究资源的需求和概述 NMR光谱法（COMD/NMR）的标题为大分子动力学中心，位于新的 约克结构生物学中心。 COMD/NMR的重点是NMR的技术开发和应用 自旋松弛和相关方法，用于表征蛋白质和核酸构象动力学 生物过程，包括配体识别，变构，催化和折叠。中心挑战 由COMD/NMR解决的是要分解使用高级NMR光谱的高激活屏障 和计算方法，因此可以提供这些复杂方法的好处 更广泛的生物学研究界。 COMD/NMR是通过 技术研发（TR＆D）项目，驱动生物医学项目（DBP），协作 以及资源的服务，培训和传播组成部分。 COMD/NMR的TR＆D组件 将开发新的和扩展的方法来表征和解释大分子动力学， 强烈专注于扩大时间尺度和可访问目标的目标的一般范围。双重 整合实验，计算和理论方法的需求（在TR＆D组件中）和 与关键的生物学研究人员（在DBP和协作组件中）进行接口，使得 COMD/NMR必需。特别是，COMD/NMR解决了四个主要障碍：（i）未来技术 发展需要在样本准备，NMR硬件和方法开发方面的综合进展， 并与计算机方法集成； （ii）在多个静态磁场处访问NMR光谱仪，AS 以及诸如动态核极化和现场循环探针之类的专业设备，对于许多人来说是关键 新兴方法； （iii）将技术转移给普通生物界已受到 方法的复杂性，缺少集成管道来促进数据获取和分析 专家，以及在单个实验室中缺乏基础设施来翻译这些方法供一般使用；和 （iv）从这些方法中受益的研究计划的生物学家并不意识到这种潜力和 缺乏将这些方法纳入自己的研究中的专业知识。技术发展最多 有效地受到挑战应用程序的有效；因此，COMD/NMR将与 DBP的杰出本地和国家调查员旨在激励和测试新兴技术。这 COMD/NMR的资源将通过生物医学研究社区广泛使用 协作和服务活动以及广泛的培训和传播计划。基本 COMD/NMR的研究发展将影响人类健康的各种生物学研究 相关性，包括退化性疾病，代谢性疾病和癌症。