timsTOF fleX with MALDI-2 for Advanced Mass Spectrometry Imaging

timsTOF fleX 与 MALDI-2 用于高级质谱成像

• 批准号: 10190407
• 负责人: Kristine Glunde
• 金额: $ 129.16万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-04-15 至 2022-10-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10190407
• 关键词: Anti-Retroviral Agents; Biochemistry; Books; Communicable Diseases; Computer software; Core Facility; Data Analyses; Funding; Grant; Human Resources; Image; Imaging Device; Immunotherapy; Infrastructure; Institution; Lasers; Lipids; Malignant Neoplasms; Maryland; Mass Spectrum Analysis; Medical; Minor; Molecular; Neurology; Neurosciences; New York; Oncology; Outcomes Research; Pediatrics; Peptides; Pharmaceutical Preparations; Pharmacology; Plastic Surgical Procedures; Polysaccharides; Preparation; Principal Investigator; Proteins; Radiology Specialty; Research Personnel; Research Project Grants; Resolution; Resources; Running; S10 grant; Sampling; Signal Transduction; Spatial Distribution; Spectrometry; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Time; Tissues; United States National Institutes of Health; Universities; cost; drug distribution; experience; instrument; instrumentation; ion mobility; ionization; operation; tool

With this high-end instrumentation grant (HEI), we apply for funds to purchase a Bruker timsTOF fleX MALDI-2 instrument for advanced matrix-assisted laser desorption/ionization (MALDI) imaging. This mass spectrometry imaging (MSI) instrument is capable of fast, high-spatial-resolution, high-spectral separation MALDI imaging, which is enabled by trapped ion mobility spectrometry (TIMS) combined with mass spectrometry in imaging mode. This instrument comes with the cutting-edge addition of a second post-ionization laser for MALDI-2 to boost signal intensity for increased sensitivity and spatial resolution. These technological improvements make the timsTOF fleX MALDI-2 MSI instrument an attractive molecular discovery tool with unprecedented molecular depth and coverage for visualizing the spatial distributions of drugs, metabolites, lipids, tryptic peptides, proteins, and N-glycans from tissue sections. The timsTOF fleX MALDI-2 will be placed in the Johns Hopkins Medical Institutions (JHMI) Applied Imaging Mass Spectrometry (AIMS) Core facility, established and directed by Dr. Glunde, the principal investigator of this HEI S10 application, who has 15 years of experience in MALDI imaging. A group of 10 NIH-funded investigators, along with Dr. Glunde, will comprise a dedicated group of major users, whose projects will greatly benefit from the availability of the Bruker timsTOF fleX MALDI-2 MSI instrument. Each major user is requesting 7-10% of instrument time. These major users are supported by a total of 14 NIH-funded R01/R21/R35 research grants on whose research outcomes the timsTOF fleX MALDI-2 instrument would have a transformative impact. They are spread across 8 Departments, which include the Departments of Radiology, Oncology, Pharmacology, Pediatrics, Infectious Disease, Biological Chemistry, Neurology, and Plastic Surgery. Several minor users will be requesting instrument time as well. Major and minor users have diverse research projects on cancer, infectious disease, antiretroviral drugs, immunotherapy, neuroscience, pediatrics, biological chemistry. Three of the users are from institutions on the East Coast, including the University of Maryland, New York University, and the University of Scranton, demonstrating that the JHMI AIMS core serves as a regional resource. The Radiology Department and JHMI have committed significant institutional funds towards the operation of this instrument, which includes the superb infrastructure of the JHMI AIMS Core facility, as well as covering running costs for the instrument, and providing dedicated technical personnel to help users with efficient sample preparation, instrument set-up and data analysis for their respective MALDI imaging applications. Online booking and billing for this instrument is available through the iLAB software in the AIMS Core.

有了这笔高端仪器赠款（HEI），我们申请资金购买Bruker Timstof Flex Maldi-2 用于晚期基质辅助激光解吸/电离（MALDI）成像的仪器。这个质谱 成像（MSI）仪器能够快速，高空间分辨率，高光谱分离MALDI成像， 通过被困的离子迁移率光谱法（TIMS）与质谱中的质谱法相结合来启用。 模式。该仪器带来了MALDI-2的第二个初级激光器的尖端添加到 提高信号强度以提高灵敏度和空间分辨率。这些技术的改进使得 Timstof Flex MALDI-2 MSI仪器一种具有前所未有的分子的吸引人的分子发现工具 深度和覆盖范围，以可视化药物，代谢物，脂质，胰蛋白酶肽的空间分布， 来自组织切片的蛋白质和N-聚糖。 Flex Maldi-2的Timstof将放置在Johns Hopkins 医疗机构（JHMI）应用成像质谱（AIMS）核心设施，建立和定向 HEI S10申请的首席研究员Glunde博士，在Maldi拥有15年的经验 成像。一组由10名NIH资助的调查员与Glunde博士一起组成的一组 主要用户的项目将大大受益于Bruker Timstof Flex MALDI-2 MSI 乐器。每个主要用户要求仪器时间的7-10％。这些主要用户得到 总共14个NIH资助的R01/R21/R35研究赠款，其研究结果是Flex MALDI-2 仪器将产生变革性的影响。它们分布在8个部门，其中包括 放射学，肿瘤学，药理学，儿科，传染病，生物学化学部门 神经病学和整形手术。几个未成年人也将要求仪器时间。专业和 未成年人有关于癌症，传染病，抗逆转录病毒药物，免疫疗法的研究项目多样 神经科学，儿科，生物化学。其中三个用户来自东海岸的机构， 包括马里兰大学，纽约大学和斯克兰顿大学，证明了这一点 JHMI目标是作为区域资源。放射科和JHMI已承诺 用于运营该工具的大量机构资金，其中包括超级基础设施 JHMI AIM AIM核心设施，以及涵盖乐器的运行成本，并提供专用 技术人员帮助用户进行有效的样品准备，仪器设置和数据分析 他们各自的MALDI成像应用。该乐器的在线预订和计费可通过 目标核心中的ILAB软件。