timsTOF fleX with MALDI-2 for Advanced Mass Spectrometry Imaging

timsTOF fleX 与 MALDI-2 用于高级质谱成像

• 批准号: 10190407
• 负责人: Kristine Glunde
• 金额: $ 129.16万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-04-15 至 2022-10-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10190407
• 关键词: Anti-Retroviral Agents; Biochemistry; Books; Communicable Diseases; Computer software; Core Facility; Data Analyses; Funding; Grant; Human Resources; Image; Imaging Device; Immunotherapy; Infrastructure; Institution; Lasers; Lipids; Malignant Neoplasms; Maryland; Mass Spectrum Analysis; Medical; Minor; Molecular; Neurology; Neurosciences; New York; Oncology; Outcomes Research; Pediatrics; Peptides; Pharmaceutical Preparations; Pharmacology; Plastic Surgical Procedures; Polysaccharides; Preparation; Principal Investigator; Proteins; Radiology Specialty; Research Personnel; Research Project Grants; Resolution; Resources; Running; S10 grant; Sampling; Signal Transduction; Spatial Distribution; Spectrometry; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Time; Tissues; United States National Institutes of Health; Universities; cost; drug distribution; experience; instrument; instrumentation; ion mobility; ionization; operation; tool

With this high-end instrumentation grant (HEI), we apply for funds to purchase a Bruker timsTOF fleX MALDI-2 instrument for advanced matrix-assisted laser desorption/ionization (MALDI) imaging. This mass spectrometry imaging (MSI) instrument is capable of fast, high-spatial-resolution, high-spectral separation MALDI imaging, which is enabled by trapped ion mobility spectrometry (TIMS) combined with mass spectrometry in imaging mode. This instrument comes with the cutting-edge addition of a second post-ionization laser for MALDI-2 to boost signal intensity for increased sensitivity and spatial resolution. These technological improvements make the timsTOF fleX MALDI-2 MSI instrument an attractive molecular discovery tool with unprecedented molecular depth and coverage for visualizing the spatial distributions of drugs, metabolites, lipids, tryptic peptides, proteins, and N-glycans from tissue sections. The timsTOF fleX MALDI-2 will be placed in the Johns Hopkins Medical Institutions (JHMI) Applied Imaging Mass Spectrometry (AIMS) Core facility, established and directed by Dr. Glunde, the principal investigator of this HEI S10 application, who has 15 years of experience in MALDI imaging. A group of 10 NIH-funded investigators, along with Dr. Glunde, will comprise a dedicated group of major users, whose projects will greatly benefit from the availability of the Bruker timsTOF fleX MALDI-2 MSI instrument. Each major user is requesting 7-10% of instrument time. These major users are supported by a total of 14 NIH-funded R01/R21/R35 research grants on whose research outcomes the timsTOF fleX MALDI-2 instrument would have a transformative impact. They are spread across 8 Departments, which include the Departments of Radiology, Oncology, Pharmacology, Pediatrics, Infectious Disease, Biological Chemistry, Neurology, and Plastic Surgery. Several minor users will be requesting instrument time as well. Major and minor users have diverse research projects on cancer, infectious disease, antiretroviral drugs, immunotherapy, neuroscience, pediatrics, biological chemistry. Three of the users are from institutions on the East Coast, including the University of Maryland, New York University, and the University of Scranton, demonstrating that the JHMI AIMS core serves as a regional resource. The Radiology Department and JHMI have committed significant institutional funds towards the operation of this instrument, which includes the superb infrastructure of the JHMI AIMS Core facility, as well as covering running costs for the instrument, and providing dedicated technical personnel to help users with efficient sample preparation, instrument set-up and data analysis for their respective MALDI imaging applications. Online booking and billing for this instrument is available through the iLAB software in the AIMS Core.

通过这笔高端仪器补助金 (HEI)，我们申请资金购买布鲁克 timsTOF fleX MALDI-2 用于先进基质辅助激光解吸/电离 (MALDI) 成像的仪器。这种质谱分析 成像（MSI）仪器能够快速、高空间分辨率、高光谱分离MALDI成像， 这是通过捕获离子迁移谱 (TIMS) 与成像中的质谱相结合实现的 模式。该仪器配备了尖端的第二个后电离激光器，用于 MALDI-2 增强信号强度以提高灵敏度和空间分辨率。这些技术改进使得 timsTOF flex MALDI-2 MSI 仪器是一款极具吸引力的分子发现工具，具有前所未有的分子 用于可视化药物、代谢物、脂质、胰蛋白酶肽、 组织切片中的蛋白质和 N-聚糖。 timsTOF fleX MALDI-2 将被放置在约翰·霍普金斯大学 医疗机构 (JHMI) 应用成像质谱 (AIMS) 核心设施，已建立和指导 作者：Glunde 博士，该 HEI S10 应用的首席研究员，拥有 15 年 MALDI 经验 成像。由 10 名 NIH 资助的研究人员和格伦德博士组成的专门小组将由 主要用户，他们的项目将大大受益于布鲁克 timsTOF fleX MALDI-2 MSI 的可用性 乐器。每个主要用户都需要 7-10% 的仪器时间。这些主要用户得到了支持 总共 14 项 NIH 资助的 R01/R21/R35 研究资助，其研究成果为 timsTOF flex MALDI-2 工具将产生变革性影响。他们分布在 8 个部门，其中包括 放射科、肿瘤科、药理学、儿科、传染病科、生物化学科、 神经病学和整形外科。一些小用户也会请求仪器时间。主要和 未成年用户有关于癌症、传染病、抗逆转录病毒药物、免疫治疗、 神经科学、儿科、生物化学。其中三名用户来自东海岸的机构， 包括马里兰大学、纽约大学和斯克兰顿大学，证明 JHMI AIMS 核心充当区域资源。放射科和 JHMI 已承诺 用于该工具运行的大量机构资金，其中包括一流的基础设施 JHMI AIMS 核心设施的费用，以及覆盖仪器的运行成本，并提供专用 技术人员帮助用户进行高效的样品制备、仪器设置和数据分析 他们各自的 MALDI 成像应用。该仪器的在线预订和计费可通过 AIMS 核心中的 iLAB 软件。