Upgrade of core imaging instrumentation and robotics for the UCSC Chemical Screening Center

UCSC 化学筛查中心核心成像仪器和机器人升级

• 批准号: 10177241
• 负责人: Robert SCOTT LOKEY
• 金额: $ 193.18万
• 依托单位: UNIVERSITY OF CALIFORNIA SANTA CRUZ
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-06-15 至 2022-06-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10177241
• 关键词: Address; African; Biological; Biomedical Research; Cell Cycle; Chemicals; Communities; Computer software; Data; Development; Employee; Ensure; Event; Faculty; Funding; Grant; Image; Image Analysis; Investigation; Laboratory Research; Libraries; Liquid substance; Methodology; Microscope; Modernization; Natural Products; Ocular Onchocerciasis; Peer Review; Pharmaceutical Preparations; Publications; RNA Splicing; Research; Robotics; Scientific Advances and Accomplishments; System; Therapeutic; Time; Tropical Disease; United States National Institutes of Health; Update; base; biological systems; circadian pacemaker; cost; improved; instrument; instrumentation; interest; medical specialties; operation; pathogen; research facility; screening; small molecule; small molecule libraries; tool

We request an integrated platform of robotics and instrumentation to update the UC Santa Cruz Chemical Screening Center (CSC), a shared research facility that has supported NIH-funded biomedical research at UC Santa Cruz since its inception in 2007. Research conducted at the CSC addresses a range of biomedical questions, including investigations of the circadian clock, host-pathogen interactions, the cell cycle, RNA splicing, tropical diseases such as African River Blindness, and development of methodologies for natural products discovery. Activity in the CSC has resulted in well over 50 peer-reviewed publications, most of which were jointly authored by faculty from different departments and specialties, and many grants have been funded based on preliminary data acquired at the CSC. HTS is central to the discovery of bioactive small molecules, either as drug leads or biological tool compounds. As the cost of liquid handling robotics and chemical libraries has declined during the past decade, HTS has become a powerful discovery tool for the academic research laboratory. While the CSC is an important shared research facility at UCSC with strong administrative support, bottlenecks exist in its current configuration that limit its potential impact on key NIH-funded projects. After 13 years of constant operation, the core liquid handling instruments in the CSC are becoming unreliable and are in need of replacement. The proposed microscope provides much better image quality, faster acquisition times, and more efficient image analysis software to the growing user base at UCSC who depend on image-based screening for their research. As interest in small molecule approaches has grown among the NIH-funded biomedical community at UCSC, we must improve the efficiency, and accuracy of our screening instrumentation. Modernizing our automated liquid handling system will not only allow the CSC to continue operations at the current throughput, but will transform the facility by enabling screening in 1536-well plates, thereby enabling the screening of a larger replacement library of 100,000 small molecules, which UCSC has offered to purchase as part of the Institutional Commitment to this proposal. Indeed, the Institutional Commitment is very strong, supporting two full-time employees for at least 5 years following installation, plus a contingency plan to ensure uninterrupted operation of the facility in the unlikely event of a budgetary shortfall. In short, the UCSC-CSC has facilitated exciting discoveries in a wide array of biological systems, many with direct therapeutic applications, and the proposed upgrade will allow this important facility to continue catalyzing NIH-funded scientific advances for years to come.

我们请求一个集成的机器人和仪器平台，以更新UC Santa Cruz Chemical 筛选中心（CSC），这是一种共享研究机构，支持UC的NIH资助的生物医学研究 自2007年成立以来圣克鲁斯（Santa Cruz）。在CSC进行的研究涉及一系列生物医学 问题，包括对昼夜节律的研究，宿主 - 病原体相互作用，细胞周期，RNA 剪接，热带疾病，例如非洲河流失明以及自然方法的发展 产品发现。 CSC的活动导致了50多个同行评审的出版物，其中大多数是 由来自不同部门和专业的教师共同撰写，许多赠款得到了资助 基于在CSC中获得的初步数据。 HTS是发现生物活性小分子的核心， 作为药物铅或生物工具化合物。作为液体处理机器人和化学库的成本 在过去的十年中，HTS已经下降了，HTS已成为学术研究的强大发现工具 实验室。尽管CSC是UCSC的重要共享研究机构，但具有强烈的行政支持，但 瓶颈存在于其当前配置中，该配置限制了其对NIH资助的关键项目的潜在影响。 13岁以后 多年的运行多年，CSC中的核心液体处理工具变得不可靠，并且是 需要更换。提出的显微镜提供了更好的图像质量，更快的获取时间， 以及更有效的图像分析软件，向UCSC上不断增长的用户群 筛选他们的研究。随着对小分子方法的兴趣在NIH资助中增长 UCSC的生物医学界，我们必须提高筛查的效率和准确性 仪器。现代化我们的自动化液体处理系统不仅将允许CSC继续 在当前吞吐量处进行操作，但会通过在1536孔板中启用筛选来改变设施， 从而使UCSC具有100,000个小分子的较大替换库进行筛选 作为对该提案的机构承诺的一部分，可以购买。确实，制度 承诺非常强大，安装后至少5年支持两名全职员工，以及 应急计划，以确保在预算不足的情况下，设施不间断地操作。 简而言之，UCSC-CSC在广泛的生物系统中促进了令人兴奋的发现 直接的治疗应用以及拟议的升级将允许这种重要的设施继续催化 NIH资助的科学进步将在未来的几年中发展。