Combined Optical Tweezers-Fluorescence Super-Resolution Microscope for Single-Molecule Biophysical Studies

用于单分子生物物理研究的光镊-荧光超分辨率组合显微镜

• 批准号: 10177000
• 负责人: Ruben L Gonzalez
• 金额: $ 133.75万
• 依托单位: COLUMBIA UNIV NEW YORK MORNINGSIDE
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-01 至 2022-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10177000
• 关键词: Area; Biochemical; Biomedical Research; Biophysics; Communities; Fluorescence; Funding; Future; Individual; Laboratories; Lasers; Microscope; New York City; Photons; Research; Research Personnel; Resolution; Scanning; Schools; United States National Institutes of Health; Universities; biophysical analysis; cost; experimental study; fluorescence microscope; instrument; instrumentation; laser tweezer; nanoscope; programs; single molecule

PROJECT SUMMARY In this application, we propose to acquire a combined optical tweezers-fluorescence super-resolution microscope for single-molecule biophysical studies from LUMICKS. The LUMICKS C-Trap will be placed in a shared instrumentation facility such that it will be available for use by the entire biomedical research community at Columbia University. Consequently, the C-Trap will impact a large number of current and future NIH-funded research programs at Columbia University. Specifically, the C-Trap will be installed, housed, operated, and maintained in the Columbia University Precision Biomolecular Characterization Facility (PBCF), a university- supported, staffed, shared instrumentation facility that will provide all Columbia University researchers with efficient, low-cost access to the C-Trap. The proposed C-Trap is a state-of-the-art instrument that will be equipped with quadrupule laser tweezers, a three-wavelength, single-photon-sensitivity laser scanning confocal fluorescence microscope, and stimulated emission depletion (STED) super-resolution fluorescence microscope (i.e., a STED nanoscope), thereby enabling the broadest range of research to be accommodated. There are currently no comparable instruments anywhere at Columbia University and there are only two C-Trap instruments in the entire New York City area. These two instruments are not located in a shared instrumentation facility and are instead located in the laboratory of individual researchers, where the instruments exclusively serve the experimental needs of the individual researcher’s laboratory and are therefore rendered unavailable to outside users. Moreover, these two instruments are configured differently from the requested instrument and have limited capabilities and, thus, cannot be used to perform the experiments proposed in the current application. Successfully establishing the proposed C-Trap in the PBCF is expected to advance the aims of more than 26 NIH-funded research programs spanning many departments and schools at Columbia University and representing an extremely broad range of biochemical, biophysical, and biomedical research programs.

项目摘要 在此应用中，我们建议获得组合的光学镊子荧光超分辨率显微镜 用于Lumicks的单分子生物物理研究。 Lumicks c-trap将放置在共享中 仪器设施，以便可以使用整个生物医学研究社区使用 哥伦比亚大学。因此，C陷阱将影响大量当前和未来的NIH资助 哥伦比亚大学的研究计划。具体而言，C-trap将安装，容纳，操作，并且 在哥伦比亚大学精确生物分子表征设施（PBCF）维护 支持，配备人员共享的仪器设施，将为所有哥伦比亚大学研究人员提供 有效的，低成本访问C-Trap。拟议的C-Trap是一种最先进的工具 配备四倍激光镊，三波长的单光敏激光扫描共焦 荧光显微镜和刺激的发射消耗（Sted）超分辨率荧光显微镜 （即，纳米镜），从而可以容纳最广泛的研究。有 目前在哥伦比亚大学任何地方都没有可比的乐器，只有两个C陷阱 整个纽约地区的乐器。这两种仪器不在共享仪器中 设施，而是位于单个研究人员的实验室中 满足个人研究人员实验室的实验需求，因此无法提供 向外部用户。此外，这两种仪器的配置方式与请求的仪器和 功能有限，因此不能用于执行电流中提出的实验 应用。预计成功建立PBCF中提出的C-TRAP将推进更多目标 超过26个NIH资助的研究计划，涉及哥伦比亚大学的许多部门和学校和 代表非常广泛的生化，生物物理和生物医学研究计划。