ShEEP Request for a High-Content Screening (HCS) Platform

ShEEP 请求建立高内涵筛选 (HCS) 平台

• 批准号: 10175276
• 负责人: Dandan Sun
• 金额: --
• 依托单位: VETERANS HEALTH ADMINISTRATION
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-10-01 至 2021-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10175276
• 关键词: Area; Biological; Biological Assay; Cell Cycle Kinetics; Cells; Collaborations; Color; Communicable Diseases; Communities; Computer software; Cultured Cells; Data; Development; Diagnosis; Discrimination; Disease; Equipment; Extramural Activities; Funding; Future; General Population; Goals; Histology; Image; Imaging Device; Incubators; Inflammatory; Investments; Laboratories; Laboratory Research; Lasers; Lung diseases; Maintenance; Malignant Neoplasms; Measures; Medical; Medical Research; Mentors; Microscope; Mus; Neurodegenerative Disorders; Organ Culture Techniques; Organoids; Physiology; Productivity; Regenerative Medicine; Research; Research Personnel; Resolution; Sampling; Scientist; Secure; Semiconductors; Sepsis; Sheep; Site; Slice; Speed; Strategic Planning; Stroke; System; Thick; Time; Tissues; Traumatic Brain Injury; Universities; Veterans; base; bone; career; chromophore; data acquisition; equipment acquisition; flexibility; fluorescence imaging; human tissue; improved; instrument; intelligent algorithm; interest; laboratory equipment; laboratory facility; lens; live cell imaging; metal oxide; nervous system disorder; programs; quantum; research facility; screening; solid state; success; synergism; tissue culture; tool; wound healing

Research at the VA Pittsburgh encompasses a broad spectrum of medical fields and is aimed at improving diagnosis and treatment of a wide range of disorders that are highly prevalent in Veterans, as well as in the general population, and that represent a priority for ORD funding and strategic planning. Major areas covered by research at the VA are neurological disorders (stroke, traumatic brain injuries, neurodegenerative disorders), pulmonary diseases, cancer, inflammatory disorders, infectious diseases, sepsis, wound healing, regenerative medicine. A common thread in the Medical Research Merit Programs is the use of cell, organ culture systems and mouse/human tissues that benefit from imaging-based analyses. In this proposal we aim to obtain funds to purchase the laser based, confocal equipped CELLINSIGHT CX7 High Content Screening Platform in order to meet the growing needs for more advanced and versatile imaging tools by the scientific community at the VA Pittsburgh. The funded research programs of these investigators require many capabilities: fixed imaging of a multitude of targets simultaneously, high-resolution discrimination of subcellular localization, cultured cells, slice tissue cultures and live cell imaging in combination with robust, reliable and versatile quantitative analyses. We identified the laser based, confocal equipped CELLINSIGHT CX7 High Content Screening Platform as a single instrument that provides the multiplexing capability using tissues, cells, organoids and intelligent algorithms for quantitative analyses. This instrument is not available either at the VA Pittsburgh or at our affiliate, the University of Pittsburgh. The acquisition of this equipment is timely with the recent opening of the VA’s state-of-the-art research facility where the equipment will be housed. A rational administrative plan has been developed to effectively manage efficient use of the platform and provide oversight for maintenance. The overall impact of the acquisition of this equipment is broad considering that 13 established investigators will be regular users of the microscope and additional PIs are expected to become users in the future as part of a growing cadre of newly VA funded scientists. By enabling VA investigators to obtain high quality data, we anticipate a seamless exchange of scientific information and an enhanced opportunity for collaborative projects between VA investigators and the University of Pittsburgh. Furthermore, two of the co-investigators are CDA awardees. The mentored stage of a clinician scientist is most critical for the successful development of their academic career. This new HCS platform will enhance their productivity by offering greater imaging quality and speed of data acquisition and help them transition from mentored to independent investigator status. Finally, the availability of this platform in the VA laboratories is expected to create opportunities for collaborations and synergize projects that have complementary focus.

VA匹兹堡的研究包括广泛的医疗领域，针对 改善了在退伍军人中高度普遍的多种疾病的诊断和治疗 就像一般人群一样，这代表了ORD资金和战略计划的优先事项。主要领域 在VA的研究中涵盖的是神经系统疾病（中风，创伤性脑损伤，神经退行性 疾病），肺部疾病，癌症，炎症性疾病，传染病，败血症，伤口愈合， 再生医学。医学研究成绩计划中的一个共同点是使用细胞，器官 培养系统和小鼠/人类组织，这些分析受益。 在此提案中，我们旨在获得购买基于激光的，配备共聚焦的Cellinsight的资金 CX7高内容筛选平台，以满足不断增长的更先进和通用的需求 VA匹兹堡科学界的成像工具。这些资助的研究计划 研究人员需要多种功能：简单地，高分辨率的众多目标的固定成像 歧视亚细胞定位，培养细胞，切片组织培养和活细胞成像 结合强大，可靠和多功能的定量分析。我们确定了基于激光的共焦 配备的CellinSight CX7高内容筛选平台作为单个仪器提供 使用组织，细胞，器官和智能算法进行定量分析的多路复用能力。这 在VA匹兹堡或我们的会员匹兹堡大学都无法获得乐器。 弗吉尼亚州最先进的研究最近开放，该设备的收购是及时的 设备将被容纳的设施。已经制定了一个理性的行政计划来有效 管理该平台的有效使用并提供维护的监督。总体影响 考虑到13位成熟的调查人员将是该设备的广泛收购 将来，显微镜和其他PI将来会成为用户，这是不断增长的新干部的一部分 VA资助的科学家。通过使VA调查人员获得高质量的数据，我们预计无缝 交换科学信息，并在VA之间提供协作项目的增强机会 调查人员和匹兹堡大学。此外，两个共同投资者是CDA获奖者。这 临床科学家的梅里德阶段对于他们的学术生涯的成功发展至关重要。 这个新的HCS平台将通过提供更高的成像质量和数据速度来提高其生产率 收购并帮助他们从修订到独立调查员身份过渡。最后，可用性 预计VA实验室中的这个平台将为合作创造机会并协同项目 具有完整的重点。