Leica SP8 Confocal Microscope

徕卡 SP8 共焦显微镜

基本信息

批准号：
10175244
负责人：
David Antonetti
金额：
$ 59.91万
依托单位：
UNIVERSITY OF MICHIGAN AT ANN ARBOR
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-04-01 至 2022-03-31
项目状态：
已结题

项目摘要

PROJECT DESCRIPTION This application requests funds to purchase a new confocal microscope to support the imaging needs of over 30 investigators at the University of Michigan. The Brehm and Kellogg Research towers of the Kellogg Eye Center house both Diabetes and Vision research scientists that have worked collaboratively to achieve high quality imaging for over 10 years with support from the NIDDK-funded Michigan Diabetes Research Center and the NEI-funded Vision Research Core. These investigators share space in the Brehm Research tower and have established a shared Imaging Facility that supports tissue and cellular processing and imaging with a strong emphasis on confocal microscopy. However, two of the three confocal microscopes are now over 10 years old and unable to provide the imaging requirements of our investigators. We propose to purchase a new confocal microscope to replace two of the existing microscopes based on four major needs common to this group of investigators. First, the system provides high speed resonant scanning to produce tiled images of large organ structures with significant improvement in image resolution compared to currently available systems. Second, the new system possesses a broad spectrum of excitation and emission capabilities with a high degree of precision to allow specific signal detection. Third, the system provides the ability to reduce background autofluorescence through time gating, a feature required by a number of our Imaging Facility users. Fourth, the system will be outfitted for live cell imaging to provide quantitative live cell assays. The new microscope will support a large group of Major Users with NIH funding addressing such fundamental biological issues as central control of feeding behavior and retinal light detection and neural circuitry as well as fundamental cell biological problems such as ion channel function and distribution, cilia formation and cell division. Other Major Users study disease pathology, including diabetes, diabetic retinopathy and neuropathy, age related macular degeneration and glaucoma. The new microscope will integrate into the shared Imaging Facility with a well-established infrastructure for providing service, training, and long-term support. Finally, the University of Michigan will provide substantial support to maintain and further equip the requested confocal microscope and the Imaging Facility. Collectively, the requested purchase will be part of a larger effort to provide imaging support to advance NIH funded research improving human health.

项目描述该申请要求资金购买新的共聚焦显微镜，以支持30多个成像需求密歇根大学的调查人员。 Kellogg眼中中心的Brehm和Kellogg研究塔为了实现高质量而努力工作的糖尿病和视力研究科学家在NIDDK资助的密歇根糖尿病研究中心和 Nei资助的视力研究核心。这些调查人员在Brehm研究塔中共享空间，并拥有建立了一个共享成像设施，该设施支持组织，细胞加工和成像强调共聚焦显微镜。但是，三个共聚焦显微镜中的两个已经超过10年并且无法提供调查人员的成像要求。我们建议购买新的共享显微镜根据这组共有的四个主要需求替换两个现有显微镜调查人员。首先，该系统提供高速谐振扫描以生成大型器官的瓷砖图像与当前可用的系统相比，图像分辨率有显着改善的结构。第二，新系统具有广泛的激发和排放能力，高度精度允许特定的信号检测。第三，系统提供了减少背景的能力自动荧光通过时间门控，这是许多成像设施使用者所需的功能。第四，系统将配备用于实时细胞成像以提供定量的活细胞测定法。新显微镜将支持一大批拥有NIH资金的主要用户，以解决诸如Central等基本生物学问题控制进食行为和视网膜光检测和神经回路以及基本细胞生物学离子通道功能和分布，纤毛形成和细胞分裂等问题。其他主要用户学习疾病病理学，包括糖尿病，糖尿病性视网膜病和神经病，与年龄相关的黄斑变性和青光眼。新的显微镜将与建立良好的提供服务，培训和长期支持的基础设施。最后，密歇根大学将提供大力支持并进一步配备所需的共聚焦显微镜和成像设施。总的来说，要求购买将是提供成像支持以推进NIH的更大努力的一部分资助的研究改善了人类健康。

项目成果

期刊论文数量（4）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Obesity-induced neuroinflammation and cognitive impairment in young adult versus middle-aged mice.

DOI：
10.1186/s12979-022-00323-7
发表时间：
2022-12-22
期刊：
IMMUNITY & AGEING
影响因子：
7.9
作者：
Henn, Rosemary E.;Elzinga, Sarah E.;Glass, Emily;Parent, Rachel;Guo, Kai;Allouch, Adam A.;Mendelson, Faye E.;Hayes, John;Webber-Davis, Ian;Murphy, Geoffery G.;Hur, Junguk;Feldman, Eva L.
通讯作者：
Feldman, Eva L.

Improved Lipofuscin Models and Quantification of Outer Segment Phagocytosis Capacity in Highly Polarized Human Retinal Pigment Epithelial Cultures.

改进的脂褐质模型和高度极化人视网膜色素上皮培养物中外节吞噬能力的量化。