Inductively Coupled Plasma Mass Spectrometer for High Sensitivity Elemental Analysis

用于高灵敏度元素分析的电感耦合等离子体质谱仪

• 批准号: 10175982
• 负责人: Martina Ralle
• 金额: $ 33.56万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-06-01 至 2022-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10175982
• 关键词: Alaska; Area; Biological; Biology; California; Canada; Cells; Client; Coupled; Funding; Health Sciences; Inductively Coupled Plasma Mass Spectrometry; International; Measures; Medicine; Mitochondria; Oregon; Organelles; Pacific Northwest; Plasma; Protocols documentation; Research Personnel; Resource Sharing; Role; S10 grant; Sampling; Services; Specimen; Speed; Sulfur; System; Techniques; Technology; Titanium; Trace Elements; United States National Institutes of Health; Universities; base; biomedical resource; detector; experience; improved; instrument; instrumentation; mass spectrometer; nanoparticle; particle; time use

Project Summary/Abstract The long term objective of this proposal is to help researchers with NIH or other federally funded projects to better understand the role of trace elements in biology or medicine. The Elemental Analysis Core (EAC) at the Oregon Health & Science University (OHSU) analyses biological specimen for their elemental content using Inductively Coupled Plasma Mass Spectrometry (ICPMS). As a University Shared Resource at OHSU, the EAC primarily serves users from OHSU and the Portland Area, however, because of our unique expertise in trace elemental analysis we also collaborate with many investigators across the US and Canada. Since inception of the EAC in 2011 we have analyzed ~14,000 samples and have steadily increased our annual sample load. Our current instrumentation, a single quadrupole inductively coupled plasma mass spectrometer, was funded by an S10 grant in 2009 and no longer meets the needs and asks for a majority of our user base. We are therefore requesting funds to replace this instrument with an Agilent 8900 triple quadrupole inductively coupled plasma mass spectrometer with single particle option. This new, state-of-the-art instrument, will greatly improve our current services and add an entirely new technique, elemental analysis of signle particles, to our portfolio. It overcomes limitations of the single quad instrument such as analyzing samples for their sulfur content or titanium concentration and improvements in detector technology offer a 10 – 100 fold increase in sensitivity. The vastly increased detector speed in combination with enhanced sensitivity allows for measuring elemental concentration for one particle at a time using a specialized sample introduction system. This will enable used to determine concentrations of trace elements in single cells or nanoparticles, for example. We will also develop protocols to detect contents in subcellular organelles such as mitochondria. Dr. Ralle, the Director of the EAC, has over 25 years of experience in the bioanalytical aspects of trace elements in biology; she is an internationally recognized expert for elemental analysis and has successfully managed the EAC. It is because of her expertise and reputation clients from across the US send samples to the EAC for analyses to OHSU. There are currently no comparable instruments with this setup anywhere in the Pacific Northwest, Alaska, or Northern California. This new instrument will create a powerful elemental analysis resource for biomedical researchers across the US.

项目摘要/摘要 该建议的长期目标是帮助NIH或其他联邦资助项目的研究人员 更好地了解痕量元素在生物学或医学中的作用。元素分析核心（EAC） 俄勒冈健康与科学大学（OHSU）使用生物标本分析其元素含量的生物标本 电感耦合等离子体质谱法（ICPM）。作为在OHSU共享资源的大学， 但是，EAC Primary为OHSU和波特兰地区的用户提供服务，因为我们的独特专业知识 痕量元素分析我们还与美国和加拿大的许多调查人员合作。自从 2011年，我们已经分析了约14,000个样本，并悄悄地增加了我们的年度 样品负载。我们当前的仪器，单个四极电感耦合等离子体质谱仪， 由2009年的S10赠款资助，不再满足需求，并要求我们的大多数用户群。 因此，我们要求资金用Agilent 8900三倍四极杆代替该乐器 带有单个颗粒选项的电感耦合等离子体质谱仪。这个新的，最先进的 仪器将大大改善我们当前的服务，并添加全新的技术，元素分析 向我们的投资组合签名粒子。它克服了单一四方仪器（例如分析）的局限性 其硫含量或钛浓度和探测器技术改进的样品可提供10 – 100倍的灵敏度增加。探测器速度大大提高，并增强灵敏度 允许使用专门样品引入一次测量一个粒子的元素浓度 系统。这将实现用于确定单细胞或纳米颗粒中微量元素的浓度，用于 例子。我们还将开发用于检测细胞细胞器（例如线粒体）中含量的方案。博士 EAC主任拉尔（Ralle）在微量元素的生物分析方面拥有超过25年的经验 生物学她是国际认可的基本分析专家，并成功地管理了 EAC。这是因为她的专业知识和声誉客户来自美国各地 分析到OHSU。当前在太平洋的任何地方都没有与此设置的可比乐器 西北，阿拉斯加或北加州。这种新仪器将创建强大的元素分析 美国生物医学研究人员的资源。