University of Washington Arboviral Research Network (UWARN)

华盛顿大学虫媒病毒研究网络 (UWARN)

基本信息

批准号：
10170249
负责人：
Michael Gale
金额：
$ 164.09万
依托单位：
UNIVERSITY OF WASHINGTON
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-05-22 至 2025-04-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10170249
关键词：
Acute Address Administrative Coordination Alphavirus Animals Antibodies Antiviral Agents Arbovirus Infections Arboviruses Area Arthropod Vectors Back Bacteriology Binding Biological Assay Biological Markers Blood Body Fluids Brazil Cell Separation Chikungunya virus Clinical Clinical Management Clinical Trials Collaborations Collection Congo Containment Data Dengue Dengue Infection Dengue Virus Detection Development Diagnosis Diagnostic Diagnostic Reagent Disease Emergency Situation Emerging Communicable Diseases Engineering Enzyme-Linked Immunosorbent Assay Epitopes Far East Fever Flavivirus Fluorescence Freezing Future Genomic approach Human Immune response Immune system Immunoglobulin G Individual Infection Infectious Diseases Research Innate Immune Response Innate Immune System Institutes Institution International Intervention Investigation Japanese Encephalitis Knowledge Laboratories Laboratory Research Lead Light Logistics Measurement Metagenomics Microcephaly Molecular Monitor Monoclonal Antibodies Natural Immunity Oropouche virus Orthobunyavirus Outcome Pakistan Parasitic infection Pathogenesis Pathway interactions Patients Population Prevalence Process Production Protein Engineering Proteins Readiness Reagent Research Resources Rift Valley Fever Risk Sampling Senegal Sensitivity and Specificity Serology Seroprevalences Site South Africa South America Specimen Statistical Data Interpretation Structure System Taiwan Technology Technology Transfer Testing The science of Mycology Therapeutic Time Universities Vaccines Variant Veterinarians Viral Viral Antibodies Viral Hemorrhagic Fevers Viral Load result Virus Virus Diseases Washington Yellow Fever ZIKA Zika Virus arthropod-borne base biobank candidate marker chikungunya cohort cross reactivity data management design experience global health global health emergency human monoclonal antibodies improved improved outcome innovation neutralizing antibody neutralizing monoclonal antibodies next generation sequencing novel novel diagnostics novel strategies pandemic disease pandemic preparedness pathogen pathogenic virus point of care population based prospective public health relevance severe dengue therapeutic target transcriptomics viral detection virology virus host interaction

项目摘要

Abstract: The United World Antiviral Research Network (UWARN, formerly referred to as the University of Washington Arboviral Research Network) will address emerging and re-emerging infectious diseases by carrying out research on arboviruses that include current high burden pathogens including dengue, chikungunya, and Zika viruses. Arboviruses also include emerging and re-emerging viruses such as Mayaro, Una, Usutu, Japanese encephalitis, yellow fever, Crimean-Congo hemorrhagic fever, and Oropouche viruses. UWARN will leverage the UW Alliance (formerly Metacenter) for Pandemic Disease Preparedness and strong research laboratory partners in Brazil, Senegal, South Africa, Pakistan and Taiwan as well as the UW Department of Global Health global reach in capacity building. The UWARN international Collaborating Partners have institution-based and population-based cohorts in place to carry out the planned research and monitor for new virus emergence. The proposed UWARN research will create new human viral-neutralizing monoclonal antibodies (Hu-nMabs) that can be used as therapeutics or diagnostics. UWARN research will also create new diagnostics, created ab initio by the Institute for Protein Design, as artificial proteins that release light when antibodies to virus are present in body fluids. Finally, UWARN research will generate an understanding of how viruses manipulate the human innate immune system, and this information will be used to design biomarkers to predict severe disease as well as to suggest host-directed therapies that could lead to better outcomes after arboviral infection. The proposed technologies are generic and could be rapidly adapted to any future emerging arbovirus threat. UWARN collaborators span virology, bacteriology, mycology, and parasitic infections, such that UWARN can respond to diverse emerging infectious diseases threats. UWARN international laboratories have advanced capabilities including biorepositories with generator-power backed-up -80C freezers, next-generation sequencing (NGS) capabilities, fluorescence cell sorting, and BSL-3 containment facilities. UWARN cohorts have biobanked samples taken from individuals during acute febrile illnesses, with known and unknown arboviral diseases, and partnership sites are poised for prospective collection of population and facility–based samples. UWARN labs will use metagenomic NGS to detect novel viral emergence in affiliated cohorts. UWARN Partners have collaborating entomologists and veterinarians to sample arthropod vectors and animal reservoirs as needed. Additional innovative capacity included the Brazil UWARN partner’s mobile van with on board NGS capability for real-time, on-site viral sequencing and sampling to viral identification in less than 18 hrs. A second partner, IRESSEF in Senegal, plans to set up a similar mobile vehicle for viral sampling and sequencing in Senegal and the Brazil group will collaborate with IRESSEF on technology transfer and best practices. UWARN partners will serve as a resource to other Emerging Infectious Diseases Research Centers (EIDRC) providing expertise in monoclonal antibodies, protein design, and host directed therapies.

摘要：联合世界抗病毒研究网络（以前称为华盛顿大学Arboviral Research网络）将通过对包括当前高烧伤病原体在内的Arbovires进行研究来解决新兴和重新出现的感染，包括粉丝，Chikungunya和Zika病毒。 arbovirus还包括新兴和重新出现的病毒，例如玛莎罗，UNA，USUTU，日本脑炎，黄热病，Crimean-Congo gongo出血热和Oropouche病毒。 UWARN将利用UW联盟（以前是Metacenter）来制备大流行病，并在巴西，塞内加尔，南非，巴基斯坦和台湾以及能力建设中全球全球全球范围的全球健康范围。 Uwarn International合作伙伴拥有基于机构和人群的同类同类，以进行计划的研究并监测新病毒出现。拟议的UWARN研究将创建新的人类病毒性中和单克隆抗体（HU-NMAB），可用作治疗或诊断。 UWARN研究还将创建新的诊断，并由蛋白质设计研究所从头开始，作为人造蛋白质，当体液中存在抗体病毒抗体时，会释放光。最后，UWARN研究将对病毒如何操纵人类先天免疫系统产生了解，并将这些信息用于设计生物标志物来预测严重的疾病，并提示以宿主为导向的疗法，这些疗法可能导致阿布维尔感染后更好的结局。拟议的技术是通用的，可以迅速适应任何未来新兴的Arbovirus威胁。 Uwarn合作者涵盖病毒学，细菌，真菌学和寄生虫感染，以使Uwarn可以应对急诊疾病的不同。 Uwarn国际实验室具有高级功能，包括具有发电机支持-80C冰柜，下一代测序（NGS）功能，荧光细胞分类和BSL-3控制设施的生物备件。 Uwarn队列在急性发热疾病期间从个体中采集了生物群样本，患有已知和未知的arboviral疾病，伙伴关系地点被毒害，以便在人口和设施的基于设施的样本中有毒性。 Uwarn Labs将使用宏基因组NGS来检测会员队列中的新病毒出现。 Uwarn Partners拥有合作的昆虫学家和兽医，根据需要采样节肢动物媒介和动物水库。额外的创新能力包括巴西UWARN合作伙伴的移动面包车，具有NGS NGS功能，可实时，现场病毒测序和采样，以在不到18小时的时间内进行病毒鉴定。第二个合作伙伴是塞内加尔的Iressef，计划在塞内加尔建立类似的移动车辆，以进行病毒采样和测序，并将与IRESSEF合作，以进行技术转移和最佳实践。 Uwarn Partners将作为其他新兴的传染病研究中心（EIDRC）的资源，从而提供单克隆抗体，蛋白质设计和宿主定向疗法的专业知识。