Center for Genetic Studies of Drug Abuse in Outbred Rats

近交系大鼠药物滥用基因研究中心

• 批准号: 10160844
• 负责人: Leah Catherine Solberg Woods
• 金额: $ 30.56万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-06-15 至 2024-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10160844
• 关键词: Animals; Behavior; Behavioral; Breeding; Data; Diabetes Mellitus; Drug abuse; Ecosystem; Ensure; Feasibility Studies; Female; Funding; Genes; Genetic; Genetic Drift; Genetic Variation; Genetic study; Goals; Grant; Heritability; Inbred Strains Rats; Inbreeding; Laboratories; Maps; National Institute of Drug Abuse; North America; Obesity; Phenotype; Pilot Projects; Population; Quantitative Trait Loci; Randomized; Rattus; Recombinants; Research; Research Personnel; Research Project Grants; Resources; Ships; Substance Use Disorder; Time; United States National Institutes of Health; Universities; Vertebral column; Wood material; experience; forest; genomic locus; heritable gene drive; male; medical schools; sample fixation; trait

Project Summary/Abstract (Core B: HS Breeding Core) The N/NIH heterogeneous stock (HS) breeding core, under the direction of Dr. Leah Solberg Woods at the Wake Forest University School of Medicine (WFUSM), will provide HS rats to Research Project 1-3. HS animals are outbred from eight inbred rat strains and maintained in a manner that minimizes inbreeding, enabling the fine-mapping of quantitative trait loci (QTL). Dr. Solberg Woods’ laboratory has maintained the HS rat colony since 2006 and hers is the only laboratory in the North America to house this unique resource. Over the past four years as part of the P50 Center for Excellence HS Breeding Core, the HS rat colony has become a national resource. We have sent rats to investigators both inside and outside the P50. Pilot and Feasibility grants funded through the P50 have led to funding for four NIDA-funded U01 grants that use HS rats. The two main goals of this core will be to 1) maintain the HS rat colony in a way that minimizes inbreeding and 2) ship a genetically diverse set of HS rats to Research Projects 1-3. To minimize inbreeding and control genetic drift, as well as to have sufficient rat to send to investigators within the P50 as well as currently funded U01 grants, the HS rat colony will increase from 64 to 92 breeder pairs and will be maintained using a randomized breeding strategy, ensuring that closely related animals are not bred together. The HS Breeding Core will ship HS rats (male and female) four or five times per year to Research Projects 1-3 over a four year period, with Research Project 1 receiving a limited number of rats in year 5. By providing a genetically diverse set of HS rats to Research Projects 1-3, this Core will provide the back-bone for the studies proposed in this P50, namely the genetic fine-mapping and identification of genes involved in behaviors related to substance use disorders.

项目摘要/摘要（核心B：HS育种核心） N/NIH异质股（HS）育种核心，在Leah Solberg Woods博士的指导下 维克森林大学医学院（WFUSM）将为研究项目1-3提供HS大鼠。 HS 动物从八个近交大鼠菌株中杂交，并以最小化杂交的方式保持 实现定量性状位置（QTL）的精细映射。 Solberg Woods博士的实验室维持了HS 自2006年以来，鼠殖民地和她的殖民地是北美唯一拥有这种独特资源的实验室。超过 作为P50卓越中心HS繁殖核心的一部分，过去四年，HS Rat Colony已成为 国家资源。我们已经将老鼠送往P50内外的调查人员。飞行员和可行性 通过P50资助的赠款已为使用HS大鼠的四个NIDA资助的U01补助金提供了资金。两个 该核心的主要目标将是1）以最小化杂种和2）运送A的方式保持HS Rat菌落 基因多样化的HS大鼠研究项目1-3。为了最大程度地减少繁殖和控制遗传漂移， 还有足够的老鼠将P50的调查人员发送给调查人员，以及目前资助的U01补助金， HS大鼠菌落将从64对增加到92对，并将使用随机育种维持 策略，确保与密切相关的动物没有被繁殖在一起。 HS育种核心将运送HS大鼠 （男性和女性）每年四到五次在四年内研究项目1-3，并进行研究 项目1在5年级接受有限数量的大鼠。 研究项目1-3，该核心将为P50中提出的研究提供背骨，即 遗传精细映射和鉴定与药物使用障碍有关的行为所涉及的基因。