The role of mosquito microRNAs in blood digestion.

蚊子 microRNA 在血液消化中的作用。

基本信息

批准号：
10159087
负责人：
ALEXANDER SIMEON RAIKHEL
金额：
$ 43.04万
依托单位：
UNIVERSITY OF CALIFORNIA RIVERSIDE
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-06-15 至 2024-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10159087
关键词：
Aedes Applications Grants Bioinformatics Blood CRISPR/Cas technology Cessation of life Chikungunya virus Culicidae Dengue Development Digestion Ecdysterone Enzymes Event Female Foundations Funding Gene Expression Genes Goals High-Throughput Nucleotide Sequencing Homologous Gene Hormone Receptor Hormones Immunoprecipitation Insecta Insulin Insulin Receptor Investigation Juvenile Hormones Knock-out Knowledge Lead Libraries Link Messenger RNA Methodology Methods Methoprene MicroRNAs Molecular Mosquito Control Mosquito-borne infectious disease Mutation Analysis Nodule Organism Pathway interactions Physiological Physiology Porifera Post-Transcriptional Regulation Process Progress Reports Proto-Oncogene Proteins c-akt Public Health RNA Interference Receptor Serine/Threonine Kinase Regulation Regulatory Pathway Reproduction Research Rest Role Site System Techniques Testing Tissues Transgenic Organisms Untranslated RNA Vectorial capacity Yellow Fever Zika Virus crosslink crosslinking and immunoprecipitation sequencing design ecdysone receptor egg gene product genetic approach innovation knock-down mosquito reproduction novel pathogen receptor reproductive transcription factor vector

Aedes Applications Grants Bioinformatics Blood CRISPR/Cas technology Cessation of life Chikungunya virus Culicidae Dengue Development Digestion Ecdysterone Enzymes Event Female Foundations Funding Gene Expression Genes Goals High-Throughput Nucleotide Sequencing Homologous Gene Hormone Receptor Hormones Immunoprecipitation Insecta Insulin Insulin Receptor Investigation Juvenile Hormones Knock-out Knowledge Lead Libraries Link Messenger RNA Methodology Methods Methoprene MicroRNAs Molecular Mosquito Control Mosquito-borne infectious disease Mutation Analysis Nodule Organism Pathway interactions Physiological Physiology Porifera Post-Transcriptional Regulation Process Progress Reports Proto-Oncogene Proteins c-akt Public Health RNA Interference Receptor Serine/Threonine Kinase Regulation Regulatory Pathway Reproduction Research Rest Role Site System Techniques Testing Tissues Transgenic Organisms Untranslated RNA Vectorial capacity Yellow Fever Zika Virus crosslink crosslinking and immunoprecipitation sequencing design ecdysone receptor egg gene product genetic approach innovation knock-down mosquito reproduction novel pathogen receptor reproductive transcription factor vector

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项目摘要

Alexander S. Raikhel PROJECT SUMMARY/ABSTRACT The damaging effect of mosquito-borne diseases is colossal, with about a million deaths and hundreds of thousands of people becoming seriously ill annually. Being the principal vector for dengue, yellow fever, chikungunya virus and recently Zika virus, the Aedes aegypti mosquito is an extremely important organism for investigation. Small non-coding microRNAs (miRNA) are responsible for posttranscriptional control of mRNAs and act as essential regulators of development. Our efforts during the previous funding cycle have revealed the role of miRNAs in controlling multiple physiological functions in female mosquitoes. Moreover, we have established a number of essential methodologies for mosquito miRNA investigations, from bioinformatics and miRNA library sequencing to genetics approaches such as CRISPR/Cas9 and GAL4/UAS. These studies have provided a foundation for further exploration of mosquito miRNAs. However, our knowledge about miRNA roles in such an essential process as female mosquito reproduction is still limited. Thus, the goal of the present grant proposal is to elucidate the involvement of miRNAs in regulatory networks governing blood-meal-activated reproductive events in female mosquitoes. The overall hypothesis is that miRNAs are critical components of these regulatory networks. We will examine the following hypotheses in Specific Aims: Aim 1. miRNAs are involved in posttranscriptional regulation of enzymes essential for blood digestion. Aim 2. miRNAs are required for the control of key factors of transcriptional networks that are necessary for orchestrating the female mosquito reproductive cycle. Aim 3. As important regulatory molecules, miRNAs are under strict control, coordinating their activity with demands of mosquito reproduction. The innovation of this proposed research rests on a comprehensive analysis of the role of miRNAs in mosquito reproduction and application of wide-ranging methodologies, such as CRISPR-Cas9 and Gal4-UAS. The significance of the present grant proposal is in establishing a novel paradigm concerning the association of miRNAs with regulatory networks controlling mosquito reproduction.

亚历山大·S·赖克尔（Alexander S. Raikhel）项目摘要/摘要蚊子传播疾病的破坏作用是巨大的，大约一百万人死亡和数百人每年有成千上万的人病重。是登革热，黄热病的主要载体 chikungunya病毒和最近的寨卡病毒，埃及埃及蚊子是极其重要的有机体调查。小型非编码microRNA（miRNA）负责mRNA的转录后控制并充当发展的基本调节者。我们在上一个资金周期中的努力揭示了 miRNA在控制女性蚊子中多种生理功能中的作用。而且，我们有建立了许多用于蚊子miRNA研究，生物信息学和 miRNA库测序与CRISPR/CAS9和GAL4/UAS等遗传学方法的测序。这些研究有为进一步探索蚊子miRNA提供了基础。但是，我们对miRNA角色的了解在这样的基本过程中，女性蚊子繁殖仍然受到限制。因此，目前赠款的目标建议是阐明miRNA参与控制血液激活的调节网络雌性蚊子的生殖事件。总体假设是miRNA是这些监管网络。我们将在特定目的中检查以下假设：目标1。miRNA是参与对血液消化必不可少的酶的转录后调节。目标2。需要miRNA 为了控制策划女性蚊子所必需的转录网络的关键因素生殖周期。目标3。作为重要的调节分子，miRNA处于严格的控制之下，协调他们的活动有蚊子繁殖的需求。这项拟议研究的创新在于对miRNA在蚊子繁殖和广泛范围应用中的作用的全面分析方法论，例如CRISPR-CAS9和GAL4-UAS。本赠款提案的意义在于建立有关miRNA与控制网络的关联的新颖范式蚊子繁殖。