Core 1: Genomics and Data Management Core

核心 1：基因组学和数据管理核心

• 批准号: 10155420
• 负责人: Paul Robson
• 金额: $ 24.75万
• 依托单位: WEILL MEDICAL COLL OF CORNELL UNIV
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-21 至 2022-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10155420
• 关键词: Antibodies; Archives; Autoimmune Diseases; Cells; Characteristics; Clinical; Complement; Cytometry; Data; Data Analyses; Data Security; Data Set; Development; Disease; Ensure; Etiology; Experimental Designs; Genomics; Heterogeneity; Human; Immune; Immunology; In Situ Hybridization; Individual; Infrastructure; Lupus; Metadata; Methods; Molecular Profiling; Normal Cell; Pathway interactions; Patients; Phenotype; Population; RNA; Research; Research Project Grants; Resolution; Reverse Transcriptase Polymerase Chain Reaction; Sampling; Stratification; Systemic Lupus Erythematosus; Technology; The Jackson Laboratory; Therapeutic; Validation; Whole Blood; Work; cell type; dashboard; data acquisition; data integration; data management; data standards; design; disorder subtype; experimental analysis; genomic data; improved; molecular subtypes; new technology; patient population; patient variability; phenotypic data; single cell technology; single-cell RNA sequencing; success; tool; transcriptomics

PROJECT SUMMARY Single cell RNA-seq and other transformative genomic technologies provide the opportunity to take human immunology to the next level of cellular detail, defining individual cells in greater orders of magnitude than previously possible. As a disease of great cellular heterogeneity, Systemic Lupus Erythematosus is ripe to be impacted by single cell technologies. The Genomics and Data Management Core (GDMC) will provide state- of-the-art genomics technologies and all data management capabilities for the proposed Center for Lupus Research. A major contribution of the GDMC will be to provide and develop the technology to analyze SLE patient samples with single cell resolution. Applying single cell technologies will help to define cell type characteristics in these patient populations, allow for specific comparisons between patient versus normal cell types and could potentially define new cell types associated with SLE and/or molecular subtypes of disease. Single cell RNA-seq will be used as a discovery tool to define cell populations and identify cell-type-specific markers. The flow and mass cytometry applications will utilize this information to develop new panels of antibodies that can be applied with greater throughput than single cell transcriptomics (i.e. on more patients and with greater numbers of cells per sample). Ultimately, the development of such a cellular phenotyping approach will enable improved stratification of SLE patients. In addition, the GDMC aims to provide the single cell tools to functionally interrogate new cell populations and pathways revealed by the resolving power of single cell transcriptomics, thus potentially uncovering new strategies for SLE therapeutics. The data management activities of the GDMC will ensure a unified and robust approach to experimental design and analysis for both research projects of the Center. GDMC will be responsible for the flow of data and information across all components of the Center and will be responsible for the submission of completed data sets/projects to the appropriate public archives. The Specific Aims for the GDMC are to 1) Provide state-of-the-art single cell transcriptomic technologies; 2) Provide state-of-the-art cellular phenotyping; and 3) Provide central storage, management and information security for data generated by the Center for Lupus Research. The core will thus fulfill essential scientific and translational needs across the Center through the application of powerful genomic technologies combined with expert data analysis and management support.

项目摘要 单细胞RNA-seq和其他变革性基因组技术提供了吸收人类的机会 免疫学至新的细胞细节，以比 以前可能。作为一种非常细胞异质性的疾病，全身性红斑狼疮已成熟为 受单细胞技术的影响。基因组学和数据管理核心（GDMC）将提供州 - 拟建狼疮中心的艺术基因组技术和所有数据管理功能 研究。 GDMC的主要贡献是提供和开发分析SLE的技术 具有单细胞分辨率的患者样品。应用单细胞技术将有助于定义细胞类型 这些患者人群的特征，可以在患者与正常细胞之间进行特定的比较 类型并可能定义与疾病的SLE和/或分子亚型相关的新细胞类型。 单细胞RNA-Seq将用作定义细胞群体并识别细胞类型特异性的发现工具 标记。流量和质量细胞仪应用程序将利用此信息来开发新的面板 可以比单细胞转录组学更大的吞吐量施用的抗体（即更多患者 每个样品的细胞数量更多）。最终，这种细胞表型的发展 方法将改善SLE患者的分层。此外，GDMC旨在提供单个 细胞工具在功能上询问新的细胞群体和途径，这是通过解决能力揭示的 单细胞转录组学，因此有可能发现SLE疗法的新策略。数据 GDMC的管理活动将确保实验设计的统一和强大的方法 该中心的两个研究项目的分析。 GDMC将负责数据和信息流 在该中心的所有组件中，将负责提交完整的数据集/项目 到适当的公共档案。 GDMC的具体目的是1）提供最先进的单个单曲 细胞转录学技术； 2）提供最先进的细胞表型； 3）提供中央 狼疮研究中心生成的数据的存储，管理和信息安全。核心 因此，将通过应用强大的 基因组技术与专家数据分析和管理支持相结合。