Core 1: Genomics and Data Management Core

核心 1：基因组学和数据管理核心

• 批准号: 10155420
• 负责人: Paul Robson
• 金额: $ 24.75万
• 依托单位: WEILL MEDICAL COLL OF CORNELL UNIV
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-21 至 2022-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10155420
• 关键词: Antibodies; Archives; Autoimmune Diseases; Cells; Characteristics; Clinical; Complement; Cytometry; Data; Data Analyses; Data Security; Data Set; Development; Disease; Ensure; Etiology; Experimental Designs; Genomics; Heterogeneity; Human; Immune; Immunology; In Situ Hybridization; Individual; Infrastructure; Lupus; Metadata; Methods; Molecular Profiling; Normal Cell; Pathway interactions; Patients; Phenotype; Population; RNA; Research; Research Project Grants; Resolution; Reverse Transcriptase Polymerase Chain Reaction; Sampling; Stratification; Systemic Lupus Erythematosus; Technology; The Jackson Laboratory; Therapeutic; Validation; Whole Blood; Work; cell type; dashboard; data acquisition; data integration; data management; data standards; design; disorder subtype; experimental analysis; genomic data; improved; molecular subtypes; new technology; patient population; patient variability; phenotypic data; single cell technology; single-cell RNA sequencing; success; tool; transcriptomics

PROJECT SUMMARY Single cell RNA-seq and other transformative genomic technologies provide the opportunity to take human immunology to the next level of cellular detail, defining individual cells in greater orders of magnitude than previously possible. As a disease of great cellular heterogeneity, Systemic Lupus Erythematosus is ripe to be impacted by single cell technologies. The Genomics and Data Management Core (GDMC) will provide state- of-the-art genomics technologies and all data management capabilities for the proposed Center for Lupus Research. A major contribution of the GDMC will be to provide and develop the technology to analyze SLE patient samples with single cell resolution. Applying single cell technologies will help to define cell type characteristics in these patient populations, allow for specific comparisons between patient versus normal cell types and could potentially define new cell types associated with SLE and/or molecular subtypes of disease. Single cell RNA-seq will be used as a discovery tool to define cell populations and identify cell-type-specific markers. The flow and mass cytometry applications will utilize this information to develop new panels of antibodies that can be applied with greater throughput than single cell transcriptomics (i.e. on more patients and with greater numbers of cells per sample). Ultimately, the development of such a cellular phenotyping approach will enable improved stratification of SLE patients. In addition, the GDMC aims to provide the single cell tools to functionally interrogate new cell populations and pathways revealed by the resolving power of single cell transcriptomics, thus potentially uncovering new strategies for SLE therapeutics. The data management activities of the GDMC will ensure a unified and robust approach to experimental design and analysis for both research projects of the Center. GDMC will be responsible for the flow of data and information across all components of the Center and will be responsible for the submission of completed data sets/projects to the appropriate public archives. The Specific Aims for the GDMC are to 1) Provide state-of-the-art single cell transcriptomic technologies; 2) Provide state-of-the-art cellular phenotyping; and 3) Provide central storage, management and information security for data generated by the Center for Lupus Research. The core will thus fulfill essential scientific and translational needs across the Center through the application of powerful genomic technologies combined with expert data analysis and management support.

项目概要 单细胞 RNA-seq 和其他变革性基因组技术提供了将人类 免疫学将细胞细节提升到新的水平，以更大的数量级定义单个细胞 以前可能。作为一种具有巨大细胞异质性的疾病，系统性红斑狼疮的治疗时机已经成熟。 受到单细胞技术的影响。基因组学和数据管理核心（GDMC）将提供状态- 为拟议的狼疮中心提供最先进的基因组学技术和所有数据管理功能 研究。 GDMC 的主要贡献将是提供和开发分析 SLE 的技术 具有单细胞分辨率的患者样本。应用单细胞技术将有助于定义细胞类型 这些患者群体的特征，允许在患者细胞与正常细胞之间进行具体比较 类型，并有可能定义与 SLE 和/或疾病分子亚型相关的新细胞类型。 单细胞 RNA-seq 将用作定义细胞群和识别细胞类型特异性的发现工具 标记。流式细胞仪和质谱仪应用程序将利用这些信息来开发新的面板 与单细胞转录组学相比，可以以更高的通量应用抗体（即更多的患者 并且每个样本的细胞数量更多）。最终，这种细胞表型分析的发展 该方法将能够改善 SLE 患者的分层。此外，GDMC 旨在提供单一 细胞工具可以功能性地询问新的细胞群和通过细胞的分辨力揭示的途径 单细胞转录组学，从而有可能揭示 SLE 治疗的新策略。数据 GDMC 的管理活动将确保实验设计和实验采用统一且稳健的方法 对中心的两个研究项目进行分析。 GDMC将负责数据和信息的流动 涵盖中心的所有组成部分，并将负责提交已完成的数据集/项目 到适当的公共档案馆。 GDMC 的具体目标是 1) 提供最先进的单一 细胞转录组技术； 2) 提供最先进的细胞表型分析； 3) 提供中央 狼疮研究中心生成的数据的存储、管理和信息安全。核心 因此，将通过应用强大的技术来满足整个中心的基本科学和转化需求 基因组技术与专家数据分析和管理支持相结合。