Core B: Animal, Cell and Tissue Culture Core

核心 B：动物、细胞和组织培养核心

基本信息

批准号：
10152475
负责人：
Andrei Seluanov
金额：
$ 45.54万
依托单位：
UNIVERSITY OF ROCHESTER
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-05-01 至 2024-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10152475
关键词：
Age Aging Animal Model Animals Beavers Biocompatible Materials Biological Biological Assay Blood Breeding Cell Culture Techniques Cell Line Cell Separation Cells Collection Comparative Study DNA DNA Damage Data Databases Development Engineering Ensure Epigenetic Process Exhibits Fibroblasts Freezing Gamma Rays Gene Expression Genes Genome Goals HAS2 gene Health Hyaluronidase IACUC Individual Intervention Longevity Maintenance Malignant Neoplasms Measures Mole Rats Molecular Mus Organism Porcupines Process Protocols documentation Quality Control RNA Rattus Reproducibility of Results Research Personnel Resistance Resources Rodent Role Sampling Squirrel Standardization Stress Testing Time Tissue Banks Tissue Harvesting Tissues Transgenic Mice Wild Type Mouse animal tissue base blind comparative genomics cost epigenome experimental study gene therapy improved inhibitor/antagonist intervention effect mouse model overexpression programs tissue culture

项目摘要

SUMMARY: The Animal, Cell and Tissue Culture Core (Core B) is responsible for providing investigators of the Program Project (PPG) with animal models, tissues and cells necessary to achieve their specific aims. Experiments of this PPG focus on identifying mechanisms responsible for the differences in longevity between species of rodents. Rodents offer a unique opportunity for such comparative study of longevity because of the over 10-fold differences in lifespans represented within a single mammalian order. For example, short-lived rodents, such as the mouse and rat, live 3-5 years, while long-lived rodents, such as the naked mole rat, blind mole rat, grey squirrel, beaver, and porcupine have maximum lifespans ranging from 21 to 32 years. To enable the study of molecular mechanisms of longevity in these animals, it is essential to provide access to biological materials from several individuals of multiple rodent species. Core B maintains and replenishes, as needed, the collection of tissues and primary cells from over 22 species of rodents. The Core provides project investigators with tissues, cells, DNA and RNA, and performs stress treatments on animals. In addition, Core B will maintain mice, naked mole rats, blind mole rat and Damaraland mole rat colonies for the use of project investigators. Considering the effort needed to collect and establish cell cultures from non-standard organisms that are not commercially available, this centralized resource saves time and effort, and reduces costs. Core B will generate and breed mouse models with longevity interventions engineered based on the longevity mechanisms discovered by project investigators in long-lived rodent species. This includes mice overexpressing naked mole rat hyaluronan synthase 2 gene (nmrHAS2 mice), beaverized SIRT6 mice, and mice treated with hyaluronidase inhibitors. Core B will perform longevity studies with these mice and analyze the effect of interventions on health. Maintaining the centralized rodent collection standardizes culture conditions, quality control, and biological samples for use across PPG projects, improves reproducibility of results, and allows the analysis of the same individual animals by several assays and projects minimizing animal use.

摘要：动物，细胞和组织培养核心（核心B）负责为研究人员提供计划项目（PPG）具有实现其特定目标所需的动物模型，组织和细胞。该PPG的实验专注于识别负责寿命差异的机制啮齿动物的种类。啮齿动物为这种寿命的比较研究提供了独特的机会在单个哺乳动物秩序中表示的寿命差异超过10倍。例如，短暂的啮齿动物，例如小鼠和老鼠，活了3 - 5年，而长寿的啮齿动物，例如裸鼠老鼠，盲人 mole大鼠，灰松鼠，海狸和豪猪的寿命范围为21至32年。启用研究这些动物寿命的分子机制的研究，必须提供生物学的机会来自多种啮齿动物的几个个体的材料。核心B维护和补充，根据需要收集来自22种啮齿动物的组织和原代细胞。核心为项目调查人员提供用组织，细胞，DNA和RNA，并对动物进行应力处理。此外，核心B将保持小鼠，裸痣大鼠，盲鼠大鼠和达马拉兰鼠大鼠菌落用于使用项目研究者。考虑到从非标准生物中收集和建立细胞培养所需的努力该集中资源在市售中节省了时间和精力，并降低了成本。核心B将产生并根据长寿机制设计的具有长寿干预措施的鼠标模型由项目调查人员发现的长寿命啮齿动物物种发现。这包括过表达裸痣的小鼠大鼠透明质酸合酶2基因（NMRHAS2小鼠），比verized Sirt6小鼠和用透明质酸酶处理的小鼠抑制剂。核心B将使用这些小鼠进行寿命研究，并分析干预措施对健康的影响。维持集中式啮齿动物收集标准化培养条件，质量控制和生物学用于跨PPG项目使用的样品，提高结果的可重复性，并允许对相同的分析单个动物通过几种测定和项目最小化动物的使用。