Molecular mechanisms of ion transport and clinical application

离子转运的分子机制及临床应用

基本信息

批准号：
10044262
负责人：
HAYAKAWA Tetsuo
金额：
$ 2.69万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B).
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

项目摘要

1. Pancreatic HCOィイD23ィエD2ィイD1-ィエD1 and fluid secretion were studied by monitoring luminal pH and luminal volume simultaneously in interlobular duct segments (diameter〜100mm) is isolated from guinea-pig pancreas. We found that HCOィイD23ィエD2ィイD1-ィエD1 uptake across the basolateral membrane is achieved largely by NaィイD1+ィエD1-HCOィイD23ィエD2ィイD1-ィエD1 cotransport, and that HCOィイD23ィエD2ィイD1-ィエD1 efflux and fluid secretion across the luminal membrane involve HCOィイD23ィエD2ィイD1-ィエD1 conductance. The location of purinoceptors in pancreatic duct cells and their role in regulating HCOィイD23ィエD2ィイD1-ィエD1 and fluid secretion was studied in microperfused duct segments. We found that both apical and basolateral nucleotides inhibit it. The efficients of arginine vasopressin (AVP) on ductal secretion were studied in isolated vascularly perfused ductal fluid secretion via VィイD21ィエD2 receptors.2. We have adopted molecular methodologies to study water transport and cloned a new aquaporin from rat liver with high … More amino-acid sequence identify to human AQP9.3. We also conduct study of water and electrolytes transport in vivo. We found that guanylin and heat-stable enterotoxin administered into the lumen of rat jejunum caused fluid secretion. Intravenous guanylin induced mucus secretion from goblet cells in rat duodenal crypts.4. We have succeeded in a continuous and simultaneous measurement of pancreatic blood flow and pancreatic secretion in conscious dog. We found that pituitary adenylate cyclase activating peptide (PACAP) and vasoactive intestinal peptide (VIP) induced vasodilatation comparable to that observed after a meal.5. We have started to investigate the relationships between mutations in cystic fibrosis transmembrane conductance regulator (CFTR) and chronic pancreatitis in Japan.6. We examined the effects of phospholipase AィイD22ィエD2 (PLAィイD22ィエD2) inhibitors on CaィイD12+ィエD1 oscillations and amylase secretion in rat pancreatic acinar cells. We found that type IV (cytosolic)PLAィイD22ィエD2 regulates CaィイD12+ィエD1 signaling and enzyme secretion. Less

1.从豚鼠胰腺中分离小叶间导管段（直径〜100mm），通过同时监测管腔pH和管腔体积来研究胰腺HCO D23 D2 D1 D1和液体分泌。基底外侧膜主要是通过NaD1+D1-HCOD23D2D1-D1 共转运，以及 HCOD23D2D1-D1 穿过管腔膜的流出和液体分泌涉及 HCO D23D2D1-D1 传导。胰管细胞中嘌呤受体的位置及其在调节中的作用。在微灌注导管段中研究了 HCO-D23-D2-D1-D1 和液体分泌，我们发现顶端和基底外侧核苷酸都会抑制它。在分离的血管灌注导管液体分泌中研究了精氨酸加压素 (AVP) 对导管分泌的效率。通过D21D2受体。2。我们采用分子方法来研究水的运输和从大鼠肝脏克隆了一种与人 AQP9.3 具有高氨基酸序列的新水通道蛋白。我们还进行了体内水和电解质转运的研究，我们发现鸟苷酸和热稳定肠毒素被注入大鼠空肠腔。静脉注射鸟苷酸诱导大鼠十二指肠隐窝杯状细胞分泌粘液。4．我们发现，垂体腺苷酸环化酶激活肽（PACAP）和血管活性肠肽（VIP）诱导的血管舒张与餐后观察到的情况相当。5。日本的囊性纤维化跨膜电导调节因子（CFTR）和慢性胰腺炎。6。 A-D22-D2 (PLA-D22-D2) 抑制剂对大鼠胰腺腺泡细胞中 Ca-D12+D1 振荡和淀粉酶分泌的影响我们发现 IV 型（胞质）PLA-D22-D2 调节 CaiD12+D1 信号传导和酶分泌。。较少的