MULTI-DISCIPLINARY STUDIES ON CEREBELLAR LONG-TERM PLASTICITY

小脑长期可塑性的多学科研究

基本信息

批准号：
09044242
负责人：
YAMAMORI Tetsuo
金额：
$ 10.3万
依托单位：
OKAZAKI NATIONAL RESEARCH INSTITUTE
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A).
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09044242/
关键词：
LONG TERM MEMORY CEREBELLAR LTD GENE XPRESSION PUCH CLAMP 遺伝子表現

项目摘要

1, Synaptic transmission and gene expression : we found that rapid protein synthesis within a few minutes is required for metabotropic glutamate receptor dependent (mGLUR) slow EPSP (in collaboration with Dr. Masao Iro).2, Cerebellar LTD in vivo lasting for several hours : we were able to record cerebeliar LTD in vivo for several hours, using macro electrode designed by Dr. Kado.3, Brain specific repetitive sequence only found in the Rattus : we found a novel type of repetitive sequence which is specifically expressed in the brain of rats, for example hippocampus, cerebellar Purkinje and olfactory mitral cells. The genes are about 150 copies per haploid genome, to our surprise, only in the rattus but no other species examined.4, Gene expression under learning behavior : we have examined c-Fos expression under an audio-visual discrimination task which has been developed by Dr. Yoshio Sakurai (Kyoto University). Under the condition where auditory cue was used, c-Fos expression was signif … More icantly enhanced. Kitsukawa et al. have examined c-Fos expression under the condition where the pattern of the pegs on wheel running was changed., finding c-Fos expression was markedly enhanced in the dorsal putamen only when the pattern was changed.5, Searching for genes expressed in the specific areas of the primate neocortex : the neocortex can be divided into the histochemically and anatomically distinct areas which play important roles in informational processing in the brain. It has been debated what extent the areas are predetermined before the thalamic innervation reached, but still not concluded. It would be useful if we can obtain the markers that are specifically expressed in the specific areas of the neocortex. Searching for this kind of genes using differential display methods, we identified one gene specifically expressed in the visual cortex in an activity dependent manner (occl ; Tochitani et al, in press) and the other gene specifically expressed in the motor cortex (gdf7 : Watakabe et al., in press). Less

1，突触传播和基因表达：我们发现，需要在几分钟之内快速蛋白质合成才能代谢谷氨酸接收器依赖（MGLUR）慢epsp（与Masao IRO博士合作）.2，Cerebellar Ltd在体内持续数小时：我们能够通过cerebeliar ltd in Dire vivo nours vivo nire vivo nire vivo nire vivo in vivo in vivo in vivo in vivo in vivo in viv，大脑特异性重复序列仅在拉图斯中发现：我们发现了一种新型的重复序列，该序列是在大鼠的大脑中特别表达的，例如海马，小脑Purkinje和嗅觉二尖瓣。使我们惊讶的是，这些基因大约是每个单倍体基因组的150份副本，但没有检查其他物种。4，在学习行为下的基因表达：我们已经检查了Yoshio Sakurai博士（Kyoto University）开发的视听歧视任务下的C-FOS表达。在使用听觉提示的条件下，C-FOS表达的意思是……更明显地增强了。 Kitsukawa等。在更改了方向盘上的钉子的模式下，在更改了C-FOS表达的条件下，只有在更改模式时发现C-FOS表达明显增强。在丘脑神经建立之前，尚未确定该地区的预定程度，但仍未得出结论。如果我们可以获得在新皮层的特定区域中特异性表达的标记，那将是有用的。在使用鉴别显示方法搜索这种基因时，我们以活动依赖性方式（cascl; tochitani等人，按下）和在运动皮质中特异性表达的另一个基因（gdf7：wakabe et al。in press in Press in Press）中特异性表达的一个基因。较少的