DISPERSAL OF MODERN HUMANS INFERRED FROM MITOCHONDRIAL DNA POLYMORPHISMS

从线粒体 DNA 多态性推断现代人类的分布

基本信息

批准号：
09440256
负责人：
HORAI Satoshi
金额：
$ 5.18万
依托单位：
THE GRADUATE UNIVERSITY FOR ADVANCED STUDIES (1998)National Institute of Genetics (1997)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09440256/
关键词：
Mitochondrial DNA D-loop region Nucleotide sequences Phylogenetic tree Ainu Nucleotide diversity Unweighted pair-grouping method Neighbor-joining method DNA多型日本人

项目摘要

Nucleotide sequences of the major noncoding (D-loop) region of human mitochondrial DNA from five Southeast Asian populations including Thais (Thais from Chiang Mai and Khon Kaen, and aboriginal Sakai), Malays and Indonesians were analyzed. Based on a comparison of 482 base pair sequences in 159 Southeast Asians, 106 different sequence types were observed. Of these, 98 were unique to their respective populations, whereas eight were shared between two or three populations. Among the shared types, six were found in common between the Thais and Indonesians. The intergenic COII/tRNA(Lys) 9 base pair deletion was observed in every Southeast Asian population except Sakai with varying frequencies. The D-loop sequence variation suggests that the deletion event occurred only once in the ancestry of Southeast Asians. Phylogenetic analysis revealed that Southeast Asian lineages were classified into at least six monophyletic clusters, though lineages from different populations were completely intermingled in the phylogenetic tree. Phylogenetic analysis of the entire human population including East Asians (Chinese, Koreans, mainland Japanese, Ryukyuans and Ainu), Europeans, Africans, and Amerinds revealed the closest genetic affinity among the two Thai (Chiang Mai and Khon Kaen) and Indonesian populations. Furthermore, net nucleotide diversity between populations suggest that overall Asians can be separated into southern and northern groups. Thus, the results of this study suggest that migration events between adjacent areas of Asia were frequent in the past.

对来自五个东南亚人群的人类线粒体 DNA 主要非编码（D 环）区域的核苷酸序列进行了分析，其中包括泰国人（来自清迈和孔敬的泰国人，以及原住民 Sakai）、马来人和印度尼西亚人。通过比较 159 名东南亚人的 482 个碱基对序列，观察到 106 种不同的序列类型。其中，98 个是各自种群所独有的，而 8 个是两个或三个种群共有的。在共有的类型中，泰国人和印度尼西亚人发现了六种共同点。除 Sakai 以外的每个东南亚人群中均观察到基因间 COII/tRNA(Lys) 9 碱基对缺失，且频率不同。 D环序列变异表明该缺失事件在东南亚人的祖先中仅发生过一次。系统发育分析表明，东南亚谱系至少被分为六个单系簇，尽管来自不同种群的谱系在系统发育树中完全混合在一起。对包括东亚人（中国人、韩国人、日本大陆人、琉球人和阿伊努人）、欧洲人、非洲人和美洲人在内的整个人类群体的系统发育分析显示，泰国（清迈和孔敬）和印度尼西亚两个人群之间的遗传亲和力最接近。此外，人群之间的净核苷酸多样性表明，整个亚洲人可以分为南部和北部群体。因此，这项研究的结果表明，过去亚洲相邻地区之间的迁徙事件很频繁。