DISPERSAL OF MODERN HUMANS INFERRED FROM MITOCHONDRIAL DNA POLYMORPHISMS

从线粒体 DNA 多态性推断现代人类的分布

基本信息

批准号：
09440256
负责人：
HORAI Satoshi
金额：
$ 5.18万
依托单位：
THE GRADUATE UNIVERSITY FOR ADVANCED STUDIES (1998)National Institute of Genetics (1997)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09440256/
关键词：
Mitochondrial DNA D-loop region Nucleotide sequences Phylogenetic tree Ainu Nucleotide diversity Unweighted pair-grouping method Neighbor-joining method DNA多型日本人

项目摘要

Nucleotide sequences of the major noncoding (D-loop) region of human mitochondrial DNA from five Southeast Asian populations including Thais (Thais from Chiang Mai and Khon Kaen, and aboriginal Sakai), Malays and Indonesians were analyzed. Based on a comparison of 482 base pair sequences in 159 Southeast Asians, 106 different sequence types were observed. Of these, 98 were unique to their respective populations, whereas eight were shared between two or three populations. Among the shared types, six were found in common between the Thais and Indonesians. The intergenic COII/tRNA(Lys) 9 base pair deletion was observed in every Southeast Asian population except Sakai with varying frequencies. The D-loop sequence variation suggests that the deletion event occurred only once in the ancestry of Southeast Asians. Phylogenetic analysis revealed that Southeast Asian lineages were classified into at least six monophyletic clusters, though lineages from different populations were completely intermingled in the phylogenetic tree. Phylogenetic analysis of the entire human population including East Asians (Chinese, Koreans, mainland Japanese, Ryukyuans and Ainu), Europeans, Africans, and Amerinds revealed the closest genetic affinity among the two Thai (Chiang Mai and Khon Kaen) and Indonesian populations. Furthermore, net nucleotide diversity between populations suggest that overall Asians can be separated into southern and northern groups. Thus, the results of this study suggest that migration events between adjacent areas of Asia were frequent in the past.

分析了来自五个东南亚人群的人线粒体DNA的主要非编码（D-Loop）区域的核苷酸序列，包括Thais（Chiang Mai和Khon Kaen的Thais，以及Malays和印度尼西亚人），Malays和印度尼西亚人。基于159个东南亚人中482个基对序列的比较，观察到了106种不同的序列类型。其中，有98人是各自的人群独有的，而在两个或三个人群之间共享了八个。在共享类型中，泰国人和印度尼西亚人之间有六种共同点。除了频率不同的Sakai以外，在每个东南亚人口中都观察到基因间COII/TRNA（LYS）9碱基对缺失。 D-Loop序列变化表明，在东南亚的血统中，缺失事件仅发生一次。系统发育分析表明，尽管来自不同人群的谱系完全混合在系统发育树中，但将东南亚血统分为至少六个单属群集。对整个人口的系统发育分析，包括东亚人（中国人，韩国人，日本人，ryukyuans和ainu），欧洲人，非洲人和阿米因特人，揭示了两个泰国人（Chiang Mai和Khon Kaen）和印度尼西亚人口之间最接近的遗传亲和力。此外，人口之间的净核苷酸多样性表明，亚洲人可以分为南部和北部群体。因此，这项研究的结果表明，过去亚洲相邻地区之间的迁移事件是经常发生的。