A new type of cytokine derived from lymphocytes affecting on nervous-endocrine system : Searching by fuzzy set cloning method

影响神经内分泌系统的一种新型淋巴细胞细胞因子：模糊集克隆法搜索

• 批准号: 09470028
• 负责人: YANAGIHARA Nobuyuki
• 金额: $ 3.2万
• 依托单位: University of Occupational and Environmental Health
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09470028/
• 关键词: Catecholamine efflux; Cytokine; Interactions among nervous-endocrine-immune system; Fuzzy set cloning; Adrenal medulla; Lymphocyte; Lymphocyte-derived factor; Bovine spleen cDNA library; カテコールアミン分泌; ファジ-セットクローニング

Cytokines were originally reported to be generated during the interaction of lymphocytes with specific antigens or non-specific mitogens and to function as communication signals among the immune systems. To investigate an interaction between lymphocytes and neurons or endocrine cells, and to seek for a new cytokine, the effects of lymphocytes and their conditioned medium on catecholamine efflux were examined in cultured bovine adrenal medullary cells. 1) Co-culture of adrenal medullary cells with lymphocytes for 3 days enhanced catecholamine efflux from the cells. 2) A conditioned medium prepared by incubation of lymphocytes for 3 days also stimulated catecholamine efflux in a time-and concentration-dependent manner. 3) Heat treatment of the conditioned medium at 60℃ and 100℃ reduced stimulatory effect to 59 and 20% of control. The stimulatory activity of the conditioned medium was almost observed in a high molecular fraction after dialysis (<8kDa cutoff) or gel filtration. 4) The cond … More itioned medium had little effect on the activity of lactate dehydrogenase in the medium of cultured adrenal medullary cells and on desipramine-sensitive [ィイD13ィエD1H] norepinephrine uptake by the cells. These findings suggest that lymphocytes release a heat-sensitive and high molecular (>8kDa) factor (lymphocyte-derived factor) which stimulates catecholamine efflux from adrenal medullary cells.Lymphocyte-derived factor(s)(LDFs) were partially purified by precipitation with 100% ammonium sulfate and followed by DEAE-cellulose column chromatography. Three peaks of active fraction were collected and pooled. Two antisera against the peak l and peak 2 were prepared and named as fuzzy antibody land antibody 2. Using the fuzzy antibody 1, 20 positive clones were isolated from bovine spleen cDNA library and partially sequenced. In these clones sequenced, the sequences of several clones (NY105, NY10, NY11) were new one when searched by the GENBANK or EMBL databases. The clones of NY16, NY18 and NY5 were found to be a new isoform of IgM, intron region for ornithine decarboxylase and one kind of serine protease, respectively. Less

最初报道了淋巴细胞与特异性抗成分或非特异性有丝分裂的相互作用，以及免疫系统之间的功能S通信信号。在培养的牛肾上腺髓质中检查了儿茶酚胺的外排。在透析（<8KDA临界值）或凝胶过滤后，在高分子分数中观察到刺激作用的刺激作用降低到59％。这些发现在培养的肾上腺介质细胞和脱哌丁胺敏感的[Lee D13 YIE D1H]的介质中。肾上腺髓质细胞的外排。淋巴细胞衍生的因子（S）（LDF）通过100％的sullfate纯化，然后收集了deae-ellularose columatography。制备称为模糊抗体抗体2。从牛脾脏cDNA文库中分离出20个阳性克隆并部分测序。或EMBL数据库

项目成果

Nobuyuki, Yanagihara: "Stimulatory effect of lymphocyte-derived factor on catecholamine efflux form cultured bovine adrenal medullary cells"Neuroscience Letters. 243. 25-28 (1998)

Nobuyuki, Yanagihara：“淋巴细胞衍生因子对培养的牛肾上腺髓质细胞儿茶酚胺流出的刺激作用”神经科学快报。

Yasuhito Uezono: "Adrenomedullin increases intracellular Ca^<2+> and inositol 1,4,5-trisphosphate in human oligodendroglial cell line KG-1C"Brain Research. 786. 230-234 (1998)

Yasuhito Uezono：“肾上腺髓质素增加人少突胶质细胞系KG-1C中的细胞内Ca 2+ 和肌醇1,4,5-三磷酸”脑研究。

Megumi, Aso: "cDNA clonig of inducer reactive protein in adult T-cell leukemia cells by applying the fuzzy cloning theory for sequencing defuzzification"Biomedical Soft Computing and Human Science. 5. 15-20 (1999)

Megumi, Aso：“通过应用模糊克隆理论进行测序去模糊化，对成人 T 细胞白血病细胞中的诱导反应蛋白进行 cDNA 克隆”《生物医学软计算与人类科学》。

Koji Kajiwara: "Atherogenic lipoproteins inhibit catecholamine secretion in cultured bovine adrenal medullary cells" Naunyn-Schimiedeberg's Archives of Pharmacology. 358. 308-314 (1998)

Koji Kajiwara：“致动脉粥样硬化脂蛋白抑制培养的牛肾上腺髓质细胞中儿茶酚胺的分泌”Naunyn-Schimiedeberg 的药理学档案。

Megumi Aso: "cDNA cloning of inducer reactive protein in adult T-cell leukemia cells by applying the fuzzy set cloning theory for sequencing defuzzification"Biomedical Soft Computing and Human Sciences. 5. 15-20 (1999)

Megumi Aso：“通过应用模糊集克隆理论进行测序去模糊化，在成人 T 细胞白血病细胞中克隆诱导反应蛋白”《生物医学软计算与人类科学》。