Roles of cells in the liver in occurrence of liver dysfunction in vivo

肝脏细胞在体内肝功能障碍发生中的作用

• 批准号: 09470263
• 负责人: NAKAGAWA Koji
• 金额: $ 9.6万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09470263/
• 关键词: Hepatic dysfunction; Obstructive jaundice; Microcirculation; Endotoxin; エンドキシン

The aim of this study was to clarify the effect of obstructive jaundice on hepatic microcirculation and Kupffer cell activities. Common bile duct ligation was performed on C57BL/6 mice to induce obstructive jaundice. One and 2 weeks after the operation, Leukocyte rolling and sticking, and hepatic sinusoidal blood flow were evaluated using hepatic intravital microscopy. Phagocytic activities were also quantitated using FITC-labeled beads. Leukocyte rolling increased significantly 1 and 2 weeks after bile duct ligation upto 409.5 % of control. Hepatic sinusoidal blood flow, evaluated as the number of sinusoids containing blood flow per microscopic field, decreased significantly associated with increased phagocytic activities after bile duct ligation. These data suggest that obstructive jaundice results in increased leukocyte adhesion and Kupffer cell activities, which may impair hepatic microcirculation, and make a host susceptible to hepatic failure.

本研究的目的是阐明梗阻性黄疸对肝脏微循环和库普弗细胞活性的影响。对 C57BL/6 小鼠进行胆总管结扎以诱导梗阻性黄疸。术后1周和2周，采用肝活体显微镜观察白细胞滚动、粘附情况以及肝窦血流情况。还使用 FITC 标记的珠子对吞噬细胞活性进行定量。胆管结扎后 1 周和 2 周，白细胞滚动显着增加，达到对照的 409.5%。肝窦血流量（以每个显微镜视野中含有血流的肝窦数量来评估）显着减少，与胆管结扎后吞噬细胞活性的增加相关。这些数据表明，阻塞性黄疸导致白细胞粘附和库普弗细胞活性增加，这可能损害肝脏微循环，并使宿主容易发生肝衰竭。

项目成果

Kazuhiro Sasada, et al.: "Augmented enhancement of in vitro production of inflammatory cytokines in peripheral blood mononuclear cells in patient"Critical Care Medicine. 27. 929-936 (1999)

Kazuhiro Sasada 等人：“增强患者外周血单核细胞中炎症细胞因子的体外产生”《重症监护医学》。

Sasada,K et al: "Augmented Enhancement of Productivities of Inflammatory cytokines in Peripheral Blood Mononuclear Cells in Patients undergoing simultaneus Resection" Critical Care Medicine. (In press). (1999)

Sasada,K 等人：“同时切除患者外周血单核细胞中炎症细胞因子生产力的增强”重症监护医学。

岡屋智久、中川宏治他: "肝類洞細胞研究の進歩"国際医書出版. 163 (1999)

冈谷智久、中川浩二等：“肝窦细胞研究进展”，Kokusai Isho Publishing 163（1999）。

Sasada K., Nakagawa K., et al.: "Augmented enhancement of productivities of inflammatory cytokines in peripheral blood mononuclear cells in patients undergoing simultaneous resection of the liver and gastrointestinal tracts"Critical Care Medicine. 27. 929

Sasada K.、Nakakawa K.等人：“同时接受肝脏和胃肠道切除的患者外周血单核细胞中炎性细胞因子生产力的增强”重症监护医学。