Molecular approaches towards control of sulfur flux in plants through selective deregulation of cysteine synthase complexes

通过选择性解除半胱氨酸合酶复合物控制植物硫通量的分子方法

• 批准号: 87777596
• 负责人: Professor Dr. Rüdiger Hell
• 金额: --
• 依托单位: Institute of Biochemistry and Biophysics
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2008
• 资助国家: 德国
• 起止时间: 2007-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/87777596?language=en
• 关键词: Molecular; approaches; towards; control; sulfur

Sulfur assimilation and cysteine biosynthesis in plants are part of the regulatory network of primary assimilation pathways that govern plant fitness and crop production. Protein complexes of cysteine synthase exist in three cellular compartments and are believed to act as regulators of primary sulfur metabolism via reversible protein-protein interaction. The aim of the project is a targeted deregulation of the formation of cysteine synthase complexes in specific compartments to study the consequences of such manipulations in planta. This will be achieved in three stages: An 'in silico' stage will include detailed analyses of the existing structural data of the two subunits of the cysteine synthase complex and the design of polypeptide candidates interfering with the interaction of subunits. During an 'in vitro' stage, the functionality of the designed peptides will be verified by biophysical and biochemical methods using recombinant purified forms of the subunits. The 'in vivo' stage will include transformation of tobacco with the pre-selected constructs and identification of genotypes with deregulated cysteine synthesis in mitochondria and chloroplasts. This will enable us to track down the structural basis of regulation within the cysteine synthase complex and its role in regulation of sulfur flux.

植物中的硫同化和半胱氨酸的生物合成是控制植物健康和作物生产的原发同化途径的调节网络的一部分。半胱氨酸合酶的蛋白质复合物存在于三个细胞室中，被认为通过可逆蛋白质 - 蛋白质相互作用充当原发性硫代谢的调节剂。该项目的目的是针对特定隔室中半胱氨酸合酶复合物形成的有针对性的放松管制，以研究植物中这种操作的后果。这将在三个阶段中实现：一个“在硅阶段”将包括对半胱氨酸合酶复合物的两个亚基的现有结构数据的详细分析以及候选多肽候选物的设计，这些候选物的相互作用干扰了亚基的相互作用。在“体外”阶段，使用重组纯化的亚基形式通过生物物理和生化方法来验证设计肽的功能。 “体内”阶段将包括烟草的转化，并具有预选的构建体以及在线粒体和叶绿体中具有失调半胱氨酸合成的基因型的鉴定。这将使我们能够追踪半胱氨酸合酶复合物中调节的结构基础及其在调节硫通量中的作用。