In vitro study of rat visual cortical plasticity

大鼠视觉皮层可塑性的体外研究

基本信息

批准号：
09480233
负责人：
TOYAMA Keisuke
金额：
$ 8.9万
依托单位：
KYOTO PREFECTURAL UNIVERSITY OF MEDICINE
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09480233/
关键词：
PLASTICITY fast LTP slow LTP protein kinase visual cortex staurosporine protein kinase 可塑性視覚野

项目摘要

In vitro study of rat visual cortical plasticityA debate between the prenatal vs. postnatal theory has long been one of the central issues in neuroscience. Recently, it has been shown that two basic structures in the cerebral cortex, the layer and columnar organizations, depend on the gene and learning, respectively. We have been studying the plasticity responsible for learning of the columnar organization in the visual cortex by in vitro approach, investigating the plasticity as long term potentiation (LTP) of synaptic transmission in the rat visual cortical slices. We found two types of LTPs. Fast (fLTP) developed almost immediately (in 5 min.) after theta-burst stimulation of the white matter while slow LTP (sLTP) developed gradually (in one hour). The two LTPs exhibited the following distinct properties. First, the fLTP occurred in the adult as well as young rat slices, while the sLTP preferentially occurred in the young slices. Second, the induction of the fLTP was blocked by blockers of the NMDA receptors (APV) while that of the sLTP was blocked by blockers of the voltage-gated Ca channels as well as those of the protein and RNA syntheses. Third, sLTP expressed in the first and second order geniculo-cortical synapses while fLTP expressed in the axon collateral synapses in layers 5 and 6. These findings indicate that the sLTP is the substrate for the developmental plasticity underlying formation of columnar structures such as the ocular dominance and orientation columns while the fLTP is that for the adult plasticity underlying learning phenomena such as adaptation.

在产前与产后理论之间对大鼠视觉皮质可塑性辩论的体外研究长期以来一直是神经科学的中心问题之一。最近，已经表明，大脑皮层中的两个基本结构分别取决于基因和学习。我们一直在研究负责通过体外方法学习柱状组织的可塑性，并研究了大鼠视觉皮质切片中突触传播的长期增强（LTP）的可塑性。我们找到了两种类型的LTP。快速（FLTP）几乎立即（在5分钟内）在抑制白质后立即发展，而缓慢的LTP（SLTP）逐渐开发（在一小时内）。两个LTP表现出以下不同的特性。首先，FLTP发生在成年人和年轻的大鼠切片中，而SLTP则优先发生在Young Slices中。其次，FLTP的诱导被NMDA受体（APV）的阻断剂阻塞，而SLTP的诱导剂被电压门控CA通道的阻滞剂以及蛋白质和RNA合成的阻滞剂阻断。第三，SLTP以二元和二阶Geniculo-cortical突触表示，而在轴突侧支突触中表示的FLTP在5和6中表达。这些发现表明，SLTP是诸如Ocular Prainmantion和Fltp Pertientation Pertications and Fltp pertientation Pertication the Flts的柱状结构形成的发展可塑性的底物。