Application of resistance genes to root knot nematode for sweet potato breeding

根结线虫抗性基因在甘薯育种中的应用

基本信息

批准号：
09660006
负责人：
TAURA Satoru
金额：
$ 1.73万
依托单位：
Kagoshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C).
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09660006/
关键词：
Ipmoea batatas Meloidogyne incognita wild spices resistance maternal effect RAPD marker transformation plant regeneration

项目摘要

In order to apply the resistance genes of sweet potato (Ipomoea batatas) and its related wild species to rot knot nematode (Meloidogyne incognita) for breeding, the following several studies were tried and produced results.1. Inheritance of resistant in cultivars : F_1 plants from reciprocal crossing between resistant and susceptible cultivar were inoculated with root knot nematode and segregated resistant plants. Maternal effect on resistance was observed in that two cross combinations.2. Wild species classification : 93 wild species which were collected at central south America from 1956 to 1986 were classified 4 genetic groups using 44 characters. The classification coincided with a classification using RAPD markers.3. Estimation of resistant degree in wild species : Wild diploid species, I trifida (cross compatible to sweet potato) group was estimated with resistant degree by inoculation test.4. Resistant expression on different conditions : From observation on the number of invaded nematodes between different growth temperature, 25℃ and 32℃, resistant expression depending on growth temperature was recognized. And on the same observation from 6 to 72 hours after inoculation, initiation of resistant expression was different between the tested lines.5. Search for related resistant region : F_1 plants from crossing between resistant and susceptible line were inoculated and divided resistant from susceptible. PCR was done using bulked DNA from resistant lines and susceptible lines, respectively. Primers (10 or 12 mar) were used in this search. RAPD marker linked with resistance gene hasn't been found yet.6. Basic research for transformation : Efficient plant regeneration from apical meristem and protoplast were developed, and somatic cell fusion plants were released.

为了将甘薯(Ipomoea batatas)及其野生种的抗性基因应用于根结线虫(Meloidogyne incognita)的育种中，进行了以下几方面的研究并取得了成果： 1．品种F_1的抗性遗传。用根结线虫接种来自抗性品种和感病品种之间的相互杂交的植株，并在两个杂交中观察到母体对抗性的影响。 2．野生种分类：将1956年至1986年在南美洲中部采集的93种野生种按44个性状分类为4个遗传类群，与RAPD标记分类一致。3．：野生二倍体种，I trifida（与甘薯杂交亲和）组通过接种试验估计抗性程度。4．不同生长温度25℃和32℃的侵染线虫，发现抗性表达依赖于生长温度，并且在接种后6至72小时的相同观察中，各测试品系之间抗性表达的起始情况不同。相关抗性区域：接种来自抗性和敏感品系之间杂交的F_1植物，并分别使用来自抗性品系和敏感品系的大量DNA（10或. 12 mar）用于该搜索尚未发现与抗性基因连锁的RAPD标记。6．转化基础研究：开发了顶端分生组织和原生质体的高效植物再生，并释放了体细胞融合植物。