Immobilization of a Water-Soluble Host Molecule by Polyion Complexation with a Polymer

通过聚离子与聚合物络合固定水溶性主体分子

基本信息

批准号：
09650942
负责人：
TSUGE Akihiko
金额：
$ 2.18万
依托单位：
Kyushu Institute of Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09650942/
关键词：
Calixarene Host-Guest chemistry Polyion complex Synthetic bilayer membrane ホストーゲスト化学

项目摘要

Complexation of macrocyclic compounds with guest molecules in water has always attracted a major interest since it can mimic the molecular recognition which is a basic process in biological systems. Although modified cyclodextrins have been extensively investigated in terms of inclusion, recognition, and catalysis in aqueous media, versatility of their structure is limited. On the contrary water-soluble calixarenes cyclophanes have a great advantage of molecular design. If these water-soluble host molecules could be taken out from the aqueous solution without altering their properties by immobilization, it would facilitate their application as practical materials exhibiting enzyme-like functions. 1n order to accomplish immobilized calixarenes as novel functional materials, the following requirement must be satisfied ; they are not dissolved in water because they are supposed to give full function at the water interface. As the second the ionic character should be remained after immobilization because electrostatic interaction as well as hydrophobic interaction plays a decisive role as a driving force for recognition of the guest molecules. Formation of polyion complex between a cationic calix[6]arene ammonium chloride which has a long alkyl chain and an anionic chondroitin sulfate results in the precipitate in the aqueous solution. After dissolving the complex in a organic solvent, evaporation of this solution gives rise to the water-insoluble transparent film. The film is strong enough for handling and shows a binding of Methyl Orange molecule, indicating that it retains a fundamental function of the parent host molecule. Immobilization of an anionic sulfonated calix[6]arene can also be achieved by formation of polyion complex with a cationic poly (diallyldimethylammonium chloride). The film prepared from this complex exhibits the ability to incorporate Phenol Blue molecule, which suggests that the binding site still remains in the polyion complex film.

大环化合物与水中的客人分子的络合始终引起了主要兴趣，因为它可以模仿分子识别，这是生物系统中的基本过程。尽管在水性培养基中的包含，识别和催化方面对经过修改的环糊精已经进行了广泛的研究，但其结构的多功能性受到限制。相反，水溶性钙棒环烯酸酯具有分子设计的很大优势。如果这些水溶性宿主分子可以从水溶液中取出而不通过固定来改变其性质，则将促进其作为表现出类似酶功能的实用材料的应用。 1N为将固定化的钙棒烯作为新型功能材料而完成，必须满足以下要求；它们不溶于水中，因为它们应该在水界面上提供全部功能。由于第二个，因此应在固定后保持离子特性，因为静电相互作用以及疏水相互作用起着决定性的作用，作为识别来宾分子的驱动力。阳离子钙[6]芳烃氯化铵之间的Polyion复合物的形成，该芳烯具有长的烷基链和阴离子软骨素硫酸盐，导致水溶液中的沉淀物。将复合物溶解在有机溶剂中后，该溶液的蒸发会导致水不溶的透明膜。该膜足够强大用于处理，并显示出甲基橙色分子的结合，表明它保留了母体宿主分子的基本功能。固定的阴离子磺化钙[6]芳烃也可以通过与阳离子聚（二甲二甲基铵氯化物）形成Polyion络合物来实现。从该复合物制备的膜表现出掺入苯酚蓝分子的能力，这表明结合位点仍然保留在Polyion复合膜中。