STUDY OF THE ROLE OF VIRIDANS GROUP STREPTOCOCCAL EXTRACELLULAR PRODUCTS AND CELL WALL COMPONENTS ON THE INJURY OF ENDOTHELIUM

Viridans集团链球菌细胞外产物和细胞壁成分对内皮损伤作用的研究

• 批准号: 09670300
• 负责人: SAKURADA Shinsaku
• 金额: $ 0.32万
• 依托单位: NIPPON MEDICAL SCHOOL
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09670300/
• 关键词: VIRIDANS GROUP STREPTOCOCCUS; MONOCYTES; MACROPHAGES; NF-kB; lipoteichoic acid; peptidoglycan; NF-κB; 血管炎; 血管内皮細胞; サイトカイン

The monocytes-derived macrophages (MDM) and human umbilical vein endothelial cells (HUVEC) were cultured with either the crude extracellular products (ECP) of viridans group streptococci (VGS), heated wholebacterial cells, lipoteichoic acid (LTA) or the peptidoglycan isolated from the cell wall of VGS.We examined wether or not NE-KB in the MDM is activated by using electrophoretic gel mobility shift assay (EMSA) and immunofluorescence technique (IF). Neither stimulators mentioned above including whole bacterial cells directly induce the activation of NP-KB in HUVEC.The accvtivation of NP-kappaB in MDM was, however, observed within 60 min after the addition of the heated whie cells. Subsequent expression of TNF-alpha, IL-beta, IL-6 genes and the release of these cytokines in the culture supematants were confirmed by RT-PCR and ELISA, respectively. Activation of NE-KB induced peptidoglycan showed a distinct kinetics pattern compared with the case of heated whole cells. ECP and purified LTA did not directly induce NE-KB activation in the both human cells. ECP induced the release of TNF-alpha, IL-beta, IL-6 to some extent in the culture supernatants of MDM but not HUVEC.The results obteined suggested that the ECP or cell wall componentsmay induce notable inflammatory responses via monocytes or macrophages.

用Viridans链球菌（VGS）的粗细细胞外产物（ECP）培养单核细胞衍生的巨噬细胞（MDM）和人脐静脉内皮细胞（HUVEC），对全球全球菌群，lipoteichoic icicicicichoic（LTA）的隔离或隔离式培训或隔离式循环介导的细胞，以下是彼此之间的热量。通过使用电泳凝胶迁移率分析（EMSA）和免疫荧光技术（IF）激活MDM中的NE-KB。上面提到的刺激剂都没有直接诱导NP-KB在HUVEC中的激活。 RT-PCR和ELISA分别证实了TNF-ALPHA，IL-BETA，IL-6基因的TNF-Alpha，IL-BETA，IL-6基因和这些细胞因子释放的表达。与加热的全细胞相比，NE-KB诱导的肽聚糖的激活显示出明显的动力学模式。 ECP和纯化的LTA并未直接诱导两个人类细胞中的NE-KB激活。 ECP在MDM的培养上清液中诱导了TNF-Alpha，IL-BETA，IL-6的释放，但没有释放。结果表明，ECP或细胞壁成分可能通过单核细胞或巨噬细胞诱导明显的炎症反应。

项目成果

木村昭夫 他: "中等度低体温化における単球の遊走・貧食並びに殺菌能の検討" 日本救急医学会雑誌. 9・8. 332-335 (1998)

Akio Kimura 等：“中度低温期间单核细胞迁移、吞咽不足和杀菌能力的检查”日本急救医学会杂志 9·8（1998）。

Akio Kimura et al.: "Chemotaxis, phagocytosis and killing of monocytes under moderate hypothermia." JJAAM.9 (8). 332-335 (1998)

Akio Kimura 等人：“中度低温下单核细胞的趋化性、吞噬作用和杀伤作用。”