A STUDY FOR PHYSIOLOGICAL FUNCTION OF LUNG GALECTIN-8

肺半乳糖凝集素8生理功能的研究

• 批准号: 09670619
• 负责人: WASANO Kojiro
• 金额: $ 2.11万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09670619/
• 关键词: gelectins; mammalian lectin; rat; epithelial cell; recombinant; cytochemistry; Galectin; 肺; 組み換え蛋白質; GST-fusion protein; 免疫組織化学; RT-PCR

Galectin-4 ( G4 ) is a member of soluble galactoside-binding lectins found in various mammalian tissues. To determine the function of this protein within colorectal tissue, we separately produced N- and C-terminal half carbohydrate-binding domain (CBD) of rat G4 as a recombinant glutathione S-transferase (GST) fusion protein ( G4-N and G4-C ) and examined the tissue-binding site(s) of each CBD by light and electron microscopy (LM and EM). At the LM level both fusion proteins stained the intercellular borders of the surface- lining epithelial cells of colorectal mucosa, At the EM level, two proteins recognized spatially close but distinct subcellular structures. G4-N stained electron-lucent flocculent substances freely located in the intercellular spaces, whereas G4-C bound to the lateral cell membranes demarcating the intercellular spaces. From these findings, it is suggested that colorectal G4 may be involved in cross-linking the lateral cell membranes of the surface-lining epithelial cells, thereby reinforcing the epithelial integrity against mechanical stress coming from the bowel lumen.

Galectin-4（G4）是在各种哺乳动物组织中发现的可溶性半乳糖苷结合凝集素的成员。为了确定该蛋白在大肠组织中的功能，我们分别产生了大鼠G4的N-和C末端半碳水化合物结合结构域（CBD）作为重组谷胱甘肽S-转移酶S-转移酶（GST）融合蛋白（G4-N和G4-C）（G4-N和G4-C），并通过light和EMROSC（s）和EMROUCT（s）和EMROUCT（s）和EMROUCT（s）和EMROUCT（s）和EMROUCT（s）。在LM水平上，两种融合蛋白都染色了结直肠粘膜的表面上皮细胞的细胞间边界，在EM水平上，两个蛋白质在空间上识别出空间接近但独特的亚细胞结构。 G4-N染色的电子胶状锥体物质自由地位于细胞间空间中，而G4-C与侧细胞膜结合，以划定细胞间空间。从这些发现中，提出结直肠G4可能参与了表面上皮细胞的外侧细胞膜的交联，从而增强了上皮完整性，以针对来自肠腔发出的机械应力。

项目成果

KOJIRO WASANO AND YASUHIRO HIRAKAWA: "TWO DOMAINS OF RAT GALECTIN-4 BIND TO DISTINCT STRUCTURES OF THE INTERCELLULAR BORDERS OF COLORECTAL EPITHELIA" J HISTOCHE CYTOCHEM. 47. 75-82 (1999)

KOJIRO Wasano 和 Yasuhiro Hirakawa：“大鼠半乳糖凝集素 4 的两个结构域与结直肠上皮细胞间边界的不同结构结合”J Histoche CYTOCHEM。

Wasano.K., Hirakawa.Y: "Two domains of rat galectin-4 bind to distinct structures of the intercellular borders of colorectal epithelia" J.Histochem.Cytochem.47(1). 75-82 (1999)

Wasano.K.、Hirakawa.Y：“大鼠半乳糖凝集素 4 的两个结构域与结直肠上皮细胞间边界的不同结构结合”J.Histochem.Cytochem.47(1)。

Wasano,K.,Hirakawa Y.: "Two domains of rat galectin-4 bind to distinct structures of the intercellular borders of colorectal epithelia" J.Histochem Cytochem. 47(1). 75-82 (1999)

Wasano,K.,Hirakawa Y.：“大鼠半乳糖凝集素 4 的两个结构域与结直肠上皮细胞间边界的不同结构结合”J.Histochem Cytochem。