Helicobacter pylori and its association with gastric cancer

幽门螺杆菌及其与胃癌的关系

基本信息

批准号：
09670502
负责人：
TAKEDA Hiroshi
金额：
$ 1.92万
依托单位：
HOKKAIDO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09670502/
关键词：
MIF H.pylori parital cell

项目摘要

Macrophage migration inhibitory factor (MIF) is the first discovered lymphokine. it was originally identified by its ability to prevent the migration of macrophages out of capillary tubes. Since then, the expression of MIF activities has been found at a variety of inflammatory loci, suggesting its role in regulating the function of macrophages in host defence. More recent reports showed that MIF may have functions besides in the immune system. For instance, MIF mRNA and protein are expressed in anterior pituitary cells, brain, embryonic eye lens and differentiating epidermal cells, suggesting its pivotal role in the regulation of neuroendocrine system, cell growth and differentiation. Furthermore, it is possible that MIF has a role in the gastric mucosal inflammation caused by Helicobacter pylon (HP) infection and the development of gastric cancer in an inflamed gatric mcosa The aim of this study was to elucidatee the role of MIF in the patliogenesis of gastric diseases associated with … More HP infectionFirst, we examined the distribution of MIF in the gastrointestinal tract. MIF mRNA and protein were strongly expressed in the rat gastric mucoca and to a lesser extent in the oesophagus, small intestine and colon by Northern blot analysis and immunoblotting. By immunohistochemical study, positive MIF staining was confined within the cytoplasm of parietal cells. Chief cells and surface mucous cells were negative for MIF staining. Intraperitoneal injection of rat gastrin I caused a decline of MIF mRNA level. After 6 hours of injection, the level of MIF mRNA returned to the level before injection. These results suggest that MIF may play an important role in parietal cell physiology. In human gastric mucosa infected with HP, MIF was strongly expressed in infiltrating lymphocytes as well as gastric parietal cells. Finally, we examined the bood level of MIF before and after the eradication therapy for HP infection. We found that the serum MIF concentration in patients with HP infection was lower than that of HP negative control subjects, and after successful eradication therapy, MIF concentration raised to the level comparable to that of control subjects. These results suggest that the local inflammatory response to HP may have the profound influence on the immune system. Less

巨噬细胞迁移抑制因子（MIF）是第一个发现的淋巴因子。它最初是通过防止巨噬细胞从毛细管中迁移的能力来确定的。从那时起，在多种炎症基因座上发现了MIF活性的表达，这表明其在确定巨噬细胞在宿主防御中的功能中的作用。最近的报告显示，除了免疫系统外，MIF可能具有功能。例如，MIF mRNA和蛋白质在前垂体细胞，脑，胚胎眼镜和分化表皮细胞中表达，这表明其在调节神经内分泌系统，细胞生长和分化中的关键作用。 Furthermore, it is possible that MIF has a role in the gastric mucosal injection caused by Helicobacter pylon (HP) infection and the development of gastric cancer in an inflamed gatric mcosa The aim of this study was to elucidatee the role of MIF in the Patliogenesis of gastric diseases associated with … More HP infectionFirst, we examined the distribution of MIF in the gastrointestinal tract.通过北印迹分析和免疫印迹，在大鼠胃粘膜中强烈表达了MIF mRNA和蛋白质，在大鼠胃粘膜中强烈表达，小肠和结肠。通过免疫组织化学研究，将阳性MIF染色限制在顶叶细胞的细胞质内。主要细胞和表面粘液细胞为MIF染色阴性。腹膜内注射大鼠胃蛋白I导致MIF mRNA水平下降。注射6小时后，MIF mRNA的水平在注射前恢复了水平。这些结果表明，MIF可能在顶叶细胞生理中起重要作用。在感染HP的人胃粘膜中，MIF在浸润淋巴细胞和胃顶细胞中强烈表达。最后，我们检查了HP感染放射治疗前后MIF的船水平。我们发现，HP感染患者的血清MIF浓度低于HP阴性对照组受试者的血清MIF浓度，在成功放射治疗后，MIF浓度升高到与对照组相当的水平。这些结果表明，对HP的局部炎症反应可能对免疫系统产生深远的影响。较少的