Label-free volumetric microscopy for visualizing intracellular motility of tumor cell culture

用于可视化肿瘤细胞培养物细胞内运动的无标记体积显微镜

• 批准号: 22KF0058
• 负责人: 安野 嘉晃
• 金额: $ 1.41万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2023
• 资助国家: 日本
• 起止时间: 2023-03-08 至 2025-03-31
• 项目状态: 未结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22KF0058/
• 关键词: Dynamic OCT; Tumor spheroid; Anti-cancer drug test; Label-free oncology; Cancer diagnosis

In FY 2022, the proposed OCT microscope has been successfully used for the anti-cancer drug investigations of the tumor spheroid. Three anti-cancer drugs were applied to human breast cancer (MCF-7) spheroids and the dynamic OCT microscopic images revealed totally different response patterns among the applied drugs. In addition, the dynamic OCT patterns were consistent with the standard fluorescence microscopic images. The results may proof the feasibility of our proposed to perform anti-cancer drug investigations.The above mentioned results have been published as an oral and poster presentations in an international conference (Photonics West 2023, BiOS conference) and a journal paper is now under writing. Several co-authored work were published in the same conference. In addition, a co-authored paper about dynamic optical coherence tomography based evaluation of human derived alveolar organoids has been published in Biomedical Optics Express journal.Further development of our proposed microscope has been also performed during FY 2022. It includes allowing the longitudinal imaging ability of our microscope. This has been performed by integrating a cultivation chamber with the microscope to allow the simulations tissue activity imaging during cultivation. The cultivation chamber integration was successful so far. And we successfully obtained the D-OCT images though the cultivation chamber window and the cap of the well plate containing the samples. It will allow future longitudinal drug response evaluation of tumor spheroids and fresh ex-vivo tissue.

在2022财年，拟议的OCT显微镜已成功用于肿瘤球体的抗癌药物研究。将三种抗癌药应用于人类乳腺癌（MCF-7）球体，动态OCT显微镜图像显示了应用药物中完全不同的反应模式。另外，动态OCT模式与标准荧光显微镜图像一致。结果可能证明了我们提议进行抗癌药物调查的可行性。上述结果已在国际会议（Photonics West 2023，BIOS会议）和杂志论文中发表，作为口头和海报演讲发表，目前正在写作。同一会议上发表了一些共同作品的作品。此外，关于动态光学相干断层扫描的共同作者的论文，基于人类衍生的牙槽器官的评估已发表在生物医学光学快递期刊上。我们提出的显微镜的发展过程中还进行了2022财年。它包括允许我们的显微镜的纵向成像能力。这是通过将培养室与显微镜整合在一起以允许在培养过程中成像的模拟组织活性成像进行的。 到目前为止，培养室的整合取得了成功。我们成功地获得了D-OCT图像的培养室窗口和包含样品的井盘的帽子。它将允许对肿瘤球体和新鲜病毒组织的未来纵向药物反应评估。

项目成果

Non-invasive and label-free zebrafish investigations using polarization-sensitive optical coherence tomography

使用偏振敏感光学相干断层扫描进行非侵入性和无标记斑马鱼研究

• 发表时间: 2023
• 作者: Antonia Lichtenegger,Lida Zhu;Tomoko Mori;Rion Morishita;Lixuan Bian;Ibrahim Abd El-Sadek;Shuichi Makita;Satoshi Matsusaka,Makoto Kobayashi,Yoshiaki Yasuno
• 通讯作者: Satoshi Matsusaka,Makoto Kobayashi,Yoshiaki Yasuno

National Taiwan University/Chang Gung University(その他の国・地域)

国立台湾大学/长庚大学（其他国家/地区）

Medical University of Vienna(オーストリア)

维也纳医科大学（奥地利）

Low- and high-resolution volumetric dynamic optical coherence tomography of in vitro cell cultures

体外细胞培养物的低分辨率和高分辨率体积动态光学相干断层扫描

• 发表时间: 2023
• 作者: Rion Morishita;Ibrahim Abd El-Sadek;Pradipta Mukherjee;Tomoko Mori;Suzuki Toshio;Antonia Lichtengger;Lim Yi Heng;Zhu Yiqiang;Shuichi Makita;Yuki Yamamoto;Tetsuharu Nagamoto;Satoshi Matsusaka;Yoshiaki Yasuno
• 通讯作者: Yoshiaki Yasuno

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