Expression of occludin and epithelial barrier function in the corneal epithelium

角膜上皮中occludin的表达和上皮屏障功能

• 批准号: 09671800
• 负责人: WATANABE Hitoshi
• 金额: $ 2.24万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671800/
• 关键词: Occludin; Tight Junction; Cornea; Epithelium; バリア機能; タイトジャクション; ZO-1

To maintain corneal transparency, tight junction is very important. Recently, occluding, tight junction protein, was found and is thought to play a role for tight junction formation. In this study, we disclosed expression of occludin in the corneal epithelium. Occludin was expressed continuously around the border of the cells in the most apical corneal epithelial cells. In the sub-apical cell layer, occludin was observed as a dotted line pattern along the cell borders. At the basal and wing cell level, occludin was not observed. In the immunoblot analysis, occludin antibody reacted with a band at approximately 65 kD.Epithelial barrier function was evaluated anterior fluorophotometer. This device clearly showed epithelial barrier function was maintained in normal corneal epithelium. In case of gelatinous drop-like dystrophy, epithelial barrier function is injured but not so highly injured, however, it is highly disturbed in case of bullous keratopathy. It is also injured in drug-induced keratopathy, even though epithelial erosion was not present. In case of gelatinous drop-like dystrophy, occludin was expressed continuously around the border of the cells, similar to normal. In contrast, in case of bullous keratopathy. occludin was expressed, but the expression was incontinuously around the border of the cells. Epithelial barieer function is maintained when occluding expression was present continuously around the border of the cells, however, epithelial barrier function is injured when occluding expression was incontinuously present around the border of the cells.These data suggest that there is a close relation between epithelial barrier function and expression of occluding in the corneal epithelium.

为了保持角膜透明度，紧密连接非常重要。最近，发现了封闭紧密连接蛋白，并被认为在紧密连接形成中发挥作用。在这项研究中，我们披露了角膜上皮中occludin的表达。 Occludin 在最顶端角膜上皮细胞的细胞边界周围连续表达。在亚顶端细胞层中，occludin 被观察为沿着细胞边界的虚线图案。在基底细胞和翼细胞水平上，没有观察到occludin。在免疫印迹分析中，occludin 抗体与大约 65 kD 的条带发生反应。通过前部荧光光度计评估上皮屏障功能。该装置清楚地表明正常角膜上皮保持了上皮屏障功能。在凝胶状水滴状营养不良的情况下，上皮屏障功能受损，但受损程度不那么严重，然而，在大疱性角膜病变的情况下，上皮屏障功能受到高度干扰。即使不存在上皮糜烂，它也会在药物引起的角膜病中受损。在凝胶状水滴样营养不良的情况下，occludin 在细胞边缘连续表达，与正常情况相似。相反，如果是大疱性角膜病。 occludin 表达，但表达在细胞边界周围不连续。当封闭表达在细胞边界周围连续存在时，上皮屏障功能得到维持，然而，当封闭表达在细胞边界周围不连续存在时，上皮屏障功能受损。这些数据表明，上皮屏障与上皮屏障之间存在密切关系。角膜上皮闭塞的功能和表达。

项目成果

Saarin-Savolainen P, Jarvinen T, Araki-Sasaki K, Watanabe H, Urtti A: "Evaluation of cytotoxicity of various ophthalmic drugs, eye drop excipients and cyclodextrins in an immortalized human corneal epithelial cell line."Pharm Res.. 15. 1275-1280 (1998)

Saarin-Savolinen P、Jarvinen T、Araki-Sasaki K、Watanabe H、Urtti A：“在永生化人角膜上皮细胞系中评估各种眼科药物、滴眼剂赋形剂和环糊精的细胞毒性。”Pharm Res.. 15. 1275

Atsushi Suzuki: "Using a reference point and Videokerafograhy for intraoporative indentification of astigmatism axis" J Cataract Ref Surg. 23・10. 1491-1495 (1997)

Atsushi Suzuki：“使用参考点和视频角膜摄影术来识别散光轴”J Cataract Ref Surg. 23・10 (1997)。

Danjo Y, Watanabe H, Tisdale AS, George M, Tsumura T, Abelson M, Gipson IK: "Alteration of mucin in human conjunctival epithelia in dry eye."Invest Ophthalmol Vis Sci. 39. 2602-9 (1998)

Danjo Y、Watanabe H、Tisdale AS、George M、Tsumura T、Abelson M、Gipson IK：“干眼症中人类结膜上皮中粘蛋白的改变。”投资眼科 Vis Sci。

Danjo Y.,Watanabe H.et al.: "Alteration of mucin in human conjunctival epithelium in dry eye"Investigative Ophthalmology and Visual Science. 39. 2602-2609 (1998)

Danjo Y.、Watanabe H.等人：“干眼症中人结膜上皮中粘蛋白的改变”眼科和视觉科学研究。

Okada M, Yamamoto S, Tsujikawa M, Watanabe H, Inoue Y, Maeda N, Shimomura Y, Nishida K, Quantock A, Kinoshita S, Tano Y: "Two distict keratoepithelin mutations in Reis-Buckler Corneal dystrophy."Am J Ophthalmol. 126. 535-542 (1998)

Okada M、Yamamoto S、Tsujikawa M、Watanabe H、Inoue Y、Maeda N、Shimomura Y、Nishida K、Quantock A、Kinoshita S、Tano Y：“Reis-Buckler 角膜营养不良中的两个不同的角上皮蛋白突变。”Am J Ophamol。